POULTRY NUTRITION AND FEED

Effects of in Ovo Leucine Injection on Growth Performance, Intestinal Tissue Morphology and Antioxidant Capacity of Female Broilers under Chronic Heat Stress

  • CUI Yangyang ,
  • HAN Guofeng ,
  • LI Yansen ,
  • LI Chunmei
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  • Research Center for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agriculture University, Nanjing 210095, China

Received date: 2021-04-26

  Online published: 2021-11-10

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Abstract

In this study, the effects of in ovo leucine injection on the growth performance, intestinal tissue morphology and antioxidant capacity of female broilers under chronic heat stress were investigated. A total of 200 fertilized Ross 308 eggs were randomly divided into 2 groups, and the average egg weight of each group was 64.9 g. The eggs were in ovo injected with leucine aqueous solution (0.5 mL/egg, containing 9.06 mg L-leucine) or ultrapure water (0.5 mL/egg) on embryonic day (ED) 7. Female chicks were selected by feather identification and were reared at 2 days of age. At 15 days of age, twenty-four female broilers of each group were chosen and assigned 8 replicates with 3 birds per replicate. Female broilers of 2 groups were exposed to a chronic heat cyclic and continued heat stress[(30±2)℃] for 19 days (10:00-16:00 of every day). After heat stress, female broilers were reared from 1 week to 46 days of age for normal temperature. The results showed as follows:compared with the control group, 1) in ovo leucine injection had no significant effect on the growth performance of female broilers (P>0.05). 2) Spleen index of 39-day-old female broilers was extremely significantly increased by in ovo leucine injection (P<0.01). 3) The height of duodenum villus and the depth of ileum crypt of 39-day-old female broilers were extremely significantly increased (P<0.01) and the depth of jejunum crypt was extremely significantly decreased by in ovo leucine injection (P<0.01), respectively; meanwhile; in ovo leucine injection significantly or extremely significantly increased the heights of ileum and jejunum villus of 39-day-old female broilers (P<0.05 or P<0.01), and extremely significantly reduced the depth of jejunum crypt (P<0.01). 4) Serum malondialdehyde (MDA) content of 46-day-old female broilers was extremely significantly reduced (P<0.01) and serum superoxide dismutase (SOD) activity was extremely significantly increased by in ovo leucine injection (P<0.01). 5) The content of MDA in jejunum tissue of 39-day-old female broilers was reduced by 46.7% (P=0.134), and the activities of catalase (CAT) and glutathione peroxidase (GSH-Px) in jejunum tissue were increased by 4.9% (P=0.997) and 53.2% (P=0.102) by in ovo leucine injection, respectively. In conclusion, in ovo leucine injection can improve the intestinal tissue morphology, spleen index and serum antioxidant capacity of female broilers under chronic heat stress.

Cite this article

CUI Yangyang , HAN Guofeng , LI Yansen , LI Chunmei . Effects of in Ovo Leucine Injection on Growth Performance, Intestinal Tissue Morphology and Antioxidant Capacity of Female Broilers under Chronic Heat Stress[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6173 -6183 . DOI: 10.3969/j.issn.1006-267x.2021.11.018

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