Microbial Community Composition and Spatial Distribution in Ileum and Cecum of 35-Day-Old Peking Ducks

HAO Yongsheng; SHEN Zhongjian; WU Yongbao; ZHANG Bo; TANG Jing; HOU Shuishen; XIE Ming

doi:10.3969/j.issn.1006-267x.2021.11.020

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 11: 6193 - 6202

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.11.020

POULTRY NUTRITION AND FEED

Microbial Community Composition and Spatial Distribution in Ileum and Cecum of 35-Day-Old Peking Ducks

HAO Yongsheng ,
SHEN Zhongjian ,
WU Yongbao ,
ZHANG Bo ,
TANG Jing ,
HOU Shuishen ,
XIE Ming

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State Key Laboratory in Animal Nutrition, Institute of Animal Sciences of Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-03-01

Online published: 2021-11-10

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Abstract

The objective of this experiment was to study the microbial community composition and spatial distribution in ileum and cecum of 35-day-old Peking ducks. A total of 36 healthy fifteen-day-old male Peking ducks with similar body weight were randomly divided into 6 replicates with 6 ducks in each replicate. The experiment lasted for 21 days. Tow Peking ducks were randomly selected from each replicate at 35 days of age and slaughtered, and the contents of the ileum and cecum were collected as samples for high-throughput sequencing by Illumina MiSeq sequencing platform and bioinformatics analysis. The results showed as follows:1) a total of 1 268 operational taxonomic units (OTUs), 23 phyla, 53 classes, 128 orders, 211 families and 455 genera were obtained from ileum and cecum of meat ducks. 2) The Simpson index in cecum of meat ducks was significantly lower than that in ileum (P<0.05), and the Shannon index was significantly higher than that in ileum (P<0.05). 3) There were five dominant phyla (relative abundance>1%) in ileum and cecum of meat duck. Among them, the Firmicutes, Actinobacteria and Proteobacteria were the dominant phyla in ileum, the Firmicutes and Bacteroidita were the dominant phyla in cecum, and the Desulfobacterota was the specifically dominant phylum in cecum. 4) There were thirty-two dominant genera (relative abundance >1%) in ileum and cecum of meat duck. Among them, the Streptococcus, Lactobacillus and Corynebacterium were the dominant genera in ileum, and the Bacteroides and Faecalibacterium were the dominant genera in cecum. 5) The Corynebacteriaceae, Lactobacillaceae and Streptococcaceae were the dominant families in ileum of meat ducks, and the Lachnospiraceae, Bacteroidaceae and Ruminococcaceae were the dominant families in cecum of meat ducks. 6) Twenty-two differential microorganisms were obtained in ileum of meat ducks, including the Streptococcaceae, Lactobacillaceae, Corynebacteriaceae, Micrococcaceae, Aerococcaceae, Clostridiaceae, Burkholderiaceae and Enterococcaceae on family level. Thirty-two differential microorganisms were obtained in cecum of meat ducks, including Bacteroidaceae, Ruminococcaceae, Lachnospiraceae, Rikenellaceae, Oscillospiraceae, Peptostreptococcaceae, Coriobacteriaceae, Acidaminococcaceae, Butyricicoccaceae and Eubacterium coprostanoligenes group on family level. In conclusion, the microbial diversity in cecum of meat ducks is higher than that in ileum. The dominant genera in ileum of meat duck are Streptococcus, Lactobacillus and Corynebacterium which are well known as amylolytic bacteria, and the dominant genera in cecum of meat duck are Bacteroides and Faecalibacterium which are involved in the metabolism of short-chain fatty acids.

Key words： meat ducks; intestinal microbiota; spatial distribution

Cite this article

HAO Yongsheng , SHEN Zhongjian , WU Yongbao , ZHANG Bo , TANG Jing , HOU Shuishen , XIE Ming . Microbial Community Composition and Spatial Distribution in Ileum and Cecum of 35-Day-Old Peking Ducks[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6193 -6202 . DOI: 10.3969/j.issn.1006-267x.2021.11.020

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