POULTRY NUTRITION AND FEED

Changes of Serum Metabolites and Pathways during Development of Squabs

  • AN Yong ,
  • JI Feng ,
  • WANG Xuemin ,
  • WANG Zheng ,
  • ZHANG Shuai
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  • 1. College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056000, China;
    2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100089, China

Received date: 2021-04-23

  Online published: 2021-11-10

Supported by

 

Abstract

The changes of serum metabolites during the development of squabs were analyzed with the technique of liquid chromatography-mass spectrometry. Six 14-day-old healthy squabs with the body weight of (398.33±43.28) g and six 21-day-old healthy squabs with the body weight of (449.17±49.44) g were selected for wing venous blood sampling, and serum was prepared and analyzed with non-target metabolomics based on the technique of LC-MS. The differential metabolites were screened out according to the variable importance in projection (VIP) and independent sample t-test, and their pathway enrichments were analyzed. The results showed that a total of 11 538 characteristic metabolic peaks were detected in the serum of 14- and 21-day-old squabs, and 694 metabolites identified in the positive and negative ion models. After the qualitative analysis of database (self-built database, Metlin, HMDB etc.), a total of 71 differential metabolites were screened, of which 49 differential metabolites were significantly up-regulated (P<0.05), and 22 differential metabolites were significantly down-regulated (P<0.05). The KEGG pathway enrichment resulted in 29 metabolic pathways, 14 of which were significantly different (P<0.05), mainly glycerophospholipid metabolism, ether lipid metabolism, glycosylphosphatidylinositol (GPI)-anchor biosynthesis etc. The contents of serum S-adenosylmethionine, L-palmitoylcarnitine, stearoylcarnitine and some metabolites of phospholipids were significantly rosed with the increase of age of squabs (P<0.05). The above data are helpful to understand the changes of the physiological state of the squabs during the development and provide references for better satisfying their nutritional needs in production.

Cite this article

AN Yong , JI Feng , WANG Xuemin , WANG Zheng , ZHANG Shuai . Changes of Serum Metabolites and Pathways during Development of Squabs[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6203 -6212 . DOI: 10.3969/j.issn.1006-267x.2021.11.021

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