RUMINANT AND HERBIVORE NUTRITION AND FEED

Energy Metabolism Analysis of Tan Sheep with Different Residual Feed Intake

  • ZHANG Qian ,
  • WANG Junkui ,
  • LUO Fang ,
  • HE Dongqian ,
  • CHEN Liyao ,
  • LU Tongtong ,
  • WU Shaofei ,
  • ZHANG Ruixue ,
  • TAO Jinzhong
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  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2021-04-21

  Online published: 2021-11-10

Supported by

 

Abstract

The aim of this study was to reveal the changes of endogenous energy metabolites in plasma of Tan sheep under different residual feed intake (RFI), and to screen out the differential metabolites that have an effect on RFI of Tan sheep. Blood samples were collected from 16 Tan sheep with very high RFI and very low RFI, respectively, metabolomics was used to detect plasma samples. Combined with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA), differential metabolites were screened according to the criteria of multiple of variation (FC)>1.3 or <0.77, variable projection importance (VIP)>1 and P<0.05. The results showed that D-xylose-5-phosphate and L-ornithine hydrochloride were down regulated in very low RFI rams vs very high RFI rams group; adenine, citric acid and L-aspartate were up-regulated and L-citrulline was down regulated in very low RFI ewes vs very high RFI ewes group; adenine and citric acid were up-regulated and L-threonine and D-xylose-5-phosphate were down regulated in very low RFI sheep vs very high RFI sheep group. The receiver operating characteristic (ROC) curve analysis showed that the area under the ROC curve (AUC) of citric acid in very low RFI vs very high RFI ewe group was more than 0.9. In conclusion, gender can lead to different energy metabolism of Tan sheep, and citric acid is expected to be a candidate biomarker for influencing RFI of female Tan sheep.

Cite this article

ZHANG Qian , WANG Junkui , LUO Fang , HE Dongqian , CHEN Liyao , LU Tongtong , WU Shaofei , ZHANG Ruixue , TAO Jinzhong . Energy Metabolism Analysis of Tan Sheep with Different Residual Feed Intake[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6309 -6319 . DOI: 10.3969/j.issn.1006-267x.2021.11.031

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