Effects of Dietary L-Arginine or N-Carbamylglutamate on Growth Performance, Intestinal Function, Serum Biochemical Indexes and Anti-Ammonia-Nitrogen Stress Ability of Yellow Catfish (Pelteobagrus fulvidraco)

ZHAO Hongxia; QIAO Guoxian; LI Peijia; CHEN Xiaoying; CHEN Bing; HUANG Wen; CAO Junming

doi:10.3969/j.issn.1006-267x.2021.11.033

2021 , Vol. 33 >Issue 11: 6330 - 6339

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.11.033

AQUATIC ANIMAL NUTRITION AND FEED

Effects of Dietary L-Arginine or N-Carbamylglutamate on Growth Performance, Intestinal Function, Serum Biochemical Indexes and Anti-Ammonia-Nitrogen Stress Ability of Yellow Catfish (Pelteobagrus fulvidraco)

ZHAO Hongxia ,
QIAO Guoxian ,
LI Peijia ,
CHEN Xiaoying ,
CHEN Bing ,
HUANG Wen ,
CAO Junming

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1. Guangdong Key Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China of Ministry of Agriculture and Rural Affairs, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. College of Fisherier, Huazhong Agricultural University, Wuhan 430070, China;
3. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China

Received date: 2021-04-09

Online published: 2021-11-10

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Abstract

This experiment was conducted to investigate the effects of dietary L-arginine (Arg) or N-carbamylglutamate (NCG) on growth performance, intestinal function, serum biochemical indexes and anti-ammonia-nitrogen stress ability of yellow catfish (Pelteobagrus fulvidraco). A total of 480 yellow catfish with an initial average body weight of (0.98±0.03) g were randomly divided into 3 groups with 4 replicates per group and 40 catfish per replicate. Three isonitrogenous and isolipidic diets (42% protein and 7% lipid) were formulated to contain 0 (control), 0.48% L-Arg and 0.025% NCG. The experiment lasted for 56 d. The results showed as follows:compared with control group and 0.48% Arg group, dietary 0.025% NCG significantly increased the weight gain rate, activities of intestinal lipase, inducible nitric oxide synthase and serum total antioxidant capacity of yellow catfish (P<0.05). Compared with the control group and 0.025% NCG group, the supplementation of 0.48% Arg significantly increased the intestinal protease activity of yellow catfish (P<0.05). The activities of arginase, nitric oxide synthase, diamine oxidase and serum lysozyme in intestinal tract of yellow catfish in 0.025% NCG group were significantly higher than those in 0.48% Arg group (P<0.05). The intestinal Na⁺/K⁺ ATPase activity of yellow catfish in 0.48% Arg group was significantly higher than that in 0.025% NCG group (P<0.05). The serum glutathione peroxidase activity of yellow catfish in 0.025% NCG group was significantly higher than that in control group (P<0.05). The serum lysozyme activity of yellow catfish in 0.48% Arg group or 0.025% NCG group was significantly higher than that in control group (P<0.05). Compared with the control group, dietary supplementation of 0.48% Arg or 0.025% NCG significantly decreased the 48 and 72 h cumulative mortality of yellow catfish under ammonia nitrogen stress (P<0.05). The 72 h cumulative mortality of yellow catfish under ammonia nitrogen stress in 0.025% NCG group was significantly lower than that in 0.48% Arg group (P<0.05). In conclusion, the supplementation of 0.025% NCG significantly improve growth performance, immune and antioxidant capacity of yellow catfish, and the supplementation of 0.48% Arg or 0.025% NCG significantly improve intestinal function and anti-ammonia-nitrogen stress ability of yellow catfish. Combined with the comprehensive evaluation of growth performance, immune antioxidant and anti-ammonia-nitrogen stress indexes, the effect of dietary NCG supplementation is better than that of Arg.

Key words： L-arginine; N-carbamylglutamate; yellow catfish (Pelteobagrus fulvidraco); growth; intestinal health; serum biochemical indexes

Cite this article

ZHAO Hongxia , QIAO Guoxian , LI Peijia , CHEN Xiaoying , CHEN Bing , HUANG Wen , CAO Junming . Effects of Dietary L-Arginine or N-Carbamylglutamate on Growth Performance, Intestinal Function, Serum Biochemical Indexes and Anti-Ammonia-Nitrogen Stress Ability of Yellow Catfish (Pelteobagrus fulvidraco)[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6330 -6339 . DOI: 10.3969/j.issn.1006-267x.2021.11.033

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