Effects of Replacing Fish Meal with Black Soldier Fly Larvae Meal on Growth Performance, Physical Indexes, Body Composition and Nutrient Retention Rates of Micropterus salmoides

PENG Kai; XIAO Hongfa; MO Wenyan; WANG Guoxia; HUANG Yanhua

doi:10.3969/j.issn.1006-267x.2021.11.034

2021 , Vol. 33 >Issue 11: 6340 - 6348

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.11.034

AQUATIC ANIMAL NUTRITION AND FEED

Effects of Replacing Fish Meal with Black Soldier Fly Larvae Meal on Growth Performance, Physical Indexes, Body Composition and Nutrient Retention Rates of Micropterus salmoides

PENG Kai ,
XIAO Hongfa ,
MO Wenyan ,
WANG Guoxia ,
HUANG Yanhua

Expand

1. Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
3. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture in Rural Affairs, Guangzhou 510640, China;
4. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China;
5. Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received date: 2021-04-16

Online published: 2021-11-10

Supported by

Fold

Abstract

A 62-day feeding trail was conducted to evaluate the effects of replacing fish meal with black soldier fly larvae meal on growth performance, physical indexes, body composition and nutrient retention rates of Micropterus salmoides. A total of 525 juvenile Micropterus salmoides with an initial body weight about 4.9 g were divided into 5 groups with 3 replicates in each group and 35 fish in each replicate. Five isoproteic and isolipidic diets were formulated by adding black soldier fly larvae meal to replace 0 (G0, as control), 7.5% (G7.5), 15.0% (G15), 22.5% (G22.5) and 30.0% (G30) of fish meal in diets. The results showed as follows:compared with G0 group, the feed conversion ratio was significantly increased (P<0.05) and protein efficiency ratio was significantly decreased (P<0.05) in G30 group. There were no significant differences in survival rate, feed intake, final body weight, weight gain rate and specific growth rate among groups (P>0.05). The viscerosomatic index in G7.5 and G15 groups as well as the intestinesomatic index in G15, G22.5 and G30 groups were significantly increased compared with G0 group (P<0.05), but the hepatosomatic index in G30 group was significantly decreased (P<0.05). There were no significant differences in condition factor, gastric somatic index and intraperitoneal fat ratio among group (P>0.05). Whole body ash and calcium contents in G15 and G22.5 groups were significantly decreased compared with G0 group (P<0.05), but there were no significant differences in whole body moisture, crude protein, crude lipid and total phosphorus contents among groups (P>0.05). The calcium retention rate in G15 and G22.5 groups was significantly decreased compared with G0 group (P<0.05), but there were no significant differences in protein, lipid, ash and phosphorus retention rates among groups (P>0.05). In conclusion, adding black soldier fly larvae meal in the diet to replace 30.0% fish meal can significantly increase the feed conversion ratio and intestinesomatic index, but significantly reduce the protein efficiency ratio and hepatosomatic index of Micropterus salmoides. Replacement of 15.0% and 22.5% fish meal with black soldier fly larvae meal can significantly reduce the whole body ash and calcium contents and inhibit calcium retention in the whole body of Micropterus salmoides.

Key words： black soldier fly larvae meal; Micropterus salmoides; growth performance; nutrient retention

Cite this article

PENG Kai , XIAO Hongfa , MO Wenyan , WANG Guoxia , HUANG Yanhua . Effects of Replacing Fish Meal with Black Soldier Fly Larvae Meal on Growth Performance, Physical Indexes, Body Composition and Nutrient Retention Rates of Micropterus salmoides[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6340 -6348 . DOI: 10.3969/j.issn.1006-267x.2021.11.034

References

[1] AKSNES A, MUNDHEIM H, TOPPE J, et al.The effect of dietary hydroxyproline supplementation on salmon (Salmo salar L.) fed high plant protein diets[J].Aquaculture, 2008, 275(1/4):242-249.
[2] PINTO W, RONNESTAD I, DINIS M T, et al.Taurine and fish development:insights for the aquaculture industry[C]//EL IDRISSI A, L'AMOREAUX W.Taurine 8.Advances in experimental medicine and biology, Vol 776.New York:Springer, 2013:329-334.
[3] WANG G X, PENG K, HU J R, et al.Evaluation of defatted black soldier fly (Hermetia illucens L.) larvae meal as an alternative protein ingredient for juvenile Japanese seabass (Lateolabrax japonicus) diets[J].Aquaculture, 2019, 507:144-154.
[4] CUMMINS V C, RAWLES S D, THOMPSON K R, et al.Evaluation of black soldier fly (Hermetia illucens) larvae meal as partial or total replacement of marine fish meal in practical diets for Pacific white shrimp (Litopenaeus vannamei)[J].Aquaculture, 2017, 473:337-344.
[5] DUMAS A, RAGGI T, BARKHOUSE J, et al.The oil fraction and partially defatted meal of black soldier fly larvae (Hermetia illucens) affect differently growth performance, feed efficiency, nutrient deposition, blood glucose and lipid digestibility of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture, 2018, 492:24-34.
[6] GASCO L, FINKE M, VAN H A.Can diets containing insects promote animal health?[J].Journal of Insects as Food and Feed, 2018, 4(1):1-4.

[7] LOCK E R, ARSIWALLA T, WAAGBO R.Insect larvae meal as an alternative source of nutrients in the diet of Atlantic salmon (Salmo salar) postsmolt[J].Aquaculture Nutrition, 2016, 22(6):1202-1213.

[8] SEALEY W M, GAYLORD T G, BARROWS F T, et al.Sensory analysis of rainbow trout, Oncorhynchus mykiss, fed enriched black soldier fly prepupae, Hermetia illucens[J].Journal of the World Aquaculture Society, 2011, 42(1):34-45.

[9] MAGALHAES R, SANCHEZ L A, LEAL R S, et al.Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)[J].Aquaculture, 2017, 476:79-85.
[10] RENNA M, SCHIAVONE A, GAI F, et al.Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets[J].Journal of Animal Science and Biotechnology, 2017, 8:57.
[11] ZHOU J S, LIU S S, JI H, et al.Effect of replacing dietary fish meal with black soldier fly larvae meal on growth and fatty acid composition of Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition, 2018, 24(1):424-433.

[12] XIAO X P, JIN P, ZHENG L Y, et al.Effects of black soldier fly (Hermetia illucens) larvae meal protein as a fishmeal replacement on the growth and immune index of yellow catfish (Pelteobagrus fulvidraco)[J].Aquaculture Research, 2018, 49(4):1569-1577.

[13] 胡俊茹, 王国霞, 莫文艳, 等.黑水虻幼虫粉替代鱼粉对鲈鱼幼鱼生长性能、体组成、血浆生化指标和组织结构的影响[J].动物营养学报, 2018, 30(2):613-623. HU J R, WANG G X, MO W Y, et al.Effects of fish meal replacement by black soldier fly (Hermetia illucens L.) larvae meal on growth performance, body composition, plasma biochemical indexes and tissue structure of juvenile Lateolabrax japonicas[J].Chinese Journal of Animal Nutrition, 2018, 30(2):613-623.(in Chinese)
[14] 陈晓瑛, 胡俊茹, 王国霞, 等.黑水虻幼虫粉替代鱼粉对黄颡鱼幼鱼生长性能、肌肉品质及血清生化指标的影响[J].动物营养学报, 2019, 31(6):2788-2799. CHEN X Y, HU J R, WANG G X, et al.Effects of fish meal replacement by black soldier fly (Hermetia illucens) larvae meal on growth performance, serum biochemical indices and meat quality of juvenile yellow catfish (Pelteobagrus fulvidraco)[J].Chinese Journal of Animal Nutrition, 2019, 31(6):2788-2799.(in Chinese)
[15] 农业农村部渔业渔政管理局, 全国水产技术推广总站中国水产学会.2020年中国渔业统计年鉴[M].北京:中国农业出版社, 2020. Ministry of Agriculture and Rural Affairs of the People's Republic of China, National Fisheries Technology Extension Center China Society of Fisheries.2020 China fishery statistical yearbook[M].Beijing:China Agriculture Press, 2020.(in Chinese)
[16] XU X, JI H, BELGHIT I, et al.Black soldier fly larvae as a better lipid source than yellow mealworm or silkworm oils for juvenile mirror carp (Cyprinus carpio var.specularis)[J].Aquaculture, 2020, 527:735453.
[17] 王国霞, 陈冰, 孙育平, 等.脱脂亮斑扁角水虻幼虫粉替代鱼粉对黄颡鱼幼鱼生长性能、营养素沉积率、血清生化指标和消化酶活性的影响[J].水产学报, 2020, 44(6):987-998. WANG G X, CHEN B, SUN Y P, et al.Effects of replacing fish meal with defatted black soldier fly (Hermetia illucens) larvae meal on growth performance, nutrient retention, serum biochemical parameters and digestive enzymes activity of juvenile Pelteobagrus fulvidraco[J].Journal of Fisheries of China, 2020, 44(6):987-998.(in Chinese)
[18] 王国霞, 莫文艳, 范怡杰, 等.黑水虻对杂交鳢生长、肌肉组成和血清指标的影响[J].水产科学, 2019, 38(5):603-609. WANG G X, MO W Y, FAN Y J, et al.Effect of black soldier fly larvae on growth performance, muscular composition and serum indices of hybrid snakehead Channa argus×C. maculate[J].Fisheries Science, 2019, 38(5):603-609.(in Chinese)
[19] FAWOLE F J, ADEOYE A A, TIAMIYU L O, et al.Substituting fishmeal with Hermetia illucens in the diets of African catfish (Clarias gariepinus):effects on growth, nutrient utilization, haemato-physiological response, and oxidative stress biomarker[J].Aquaculture, 2020, 518:734849.
[20] LI Y, KORTNER T M, CHIKWATI E M, et al.Total replacement of fish meal with black soldier fly (Hermetia illucens) larvae meal does not compromise the gut health of Atlantic salmon (Salmo salar)[J].Aquaculture, 2020, 520:734967.
[21] 胡俊茹, 王国霞, 黄文庆, 等.黑水虻幼虫粉替代鱼粉对凡纳滨对虾幼虾生长性能、体组成、血清生化指标和抗氧化能力的影响[J].动物营养学报, 2019, 31(11):5293-5301. HU J R, WANG G X, HUANG W Q, et al.Effects of fish meal replacement by black soldier fly (Hermetia illucens) larvae meal on growth performance, body composition, serum biochemical indexes and antioxidant ability of juvenile Litopenaeus vannamei[J].Chinese Journal of Animal Nutrition, 2019, 31(11):5293-5301.(in Chinese)
[22] WANG G X, PENG K, HU J R, et al.Evaluation of defatted Hermetia illucens larvae meal for Litopenaeus vannamei:effects on growth performance, nutrition retention, antioxidant and immune response, digestive enzyme activity and hepatic morphology[J/OL].Aquaculture Nutrition, 2021.(2021-02-17)[2021-02-20].https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/anu.13240.doi:10.1111/anu.13240.
[23] KROECKEL S, HARJES A G E, ROTH I, et al.When a turbot catches a fly:evaluation of a pre-pupae meal of the black soldier fly (Hermetia illucens) as fish meal substitute-growth performance and chitin degradation in juvenile turbot (Psetta maxima)[J].Aquaculture, 2012, 364/365:345-352.
[24] CAIMI C, RENNA M, LUSSIANA C, et al.First insights on black soldier fly (Hermetia illucens L.) larvae meal dietary administration in Siberian sturgeon (Acipenser baerii Brandt) juveniles[J].Aquaculture, 2020, 515:734539.
[25] LINDSAY G J H.Adsorption of rainbow trout (Salmo gairdneri) gastric lysozymes and chitinase by cellulose and chitin[J].Aquaculture, 1984, 42(3/4):241-246.
[26] 刘兴, 孙学亮, 李连星, 等.黑水虻替代鱼粉对锦鲤生长和健康状况的影响[J].大连海洋大学学报, 2017, 32(4):422-427. LIU X, SUN X L, LI L X, et al.Effects of dietary fish meal replaced by Hermetia illucens meal on growth and health of koi carp Cyprinus carpio[J].Journal of Dalian Fisheries University, 2017, 32(4):422-427.(in Chinese)
[27] SHIAU S Y, YU Y P.Dietary supplementation of chitin and chitosan depresses growth in tilapia, Oreochromis niloticus×O.aureus[J].Aquaculture, 1999, 179(1/2/3/4):439-446.
[28] GOPALAKANNAN A, ARUL, V.Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds[J].Aquaculture, 2006, 255(1/4):179-187.
[29] OLSEN R E, SUONTAMA J, LANGMYHR E, et al.The replacement of fish meal with Antarctic krill, Euphausia superba in diets for Atlantic salmon, Salmo salar[J].Aquaculture Nutrition, 2006, 12(4):280-290.

[30] 韩星星.脱脂黑水虻虫粉在大黄鱼幼鱼配合饲料中的应用研究[D].硕士学位论文.厦门:集美大学, 2019. HAN X X.Study on the application of defatted black soldier fly larvae meal on the formulated diets for juvenile large yellow croker (Larimichthys crocea)[D].Master's Thesis.Xiamen:Jimei University, 2019.(in Chinese)
[31] LI S L, JI H, ZHANG B X, et al.Defatted black soldier fly (Hermetia illucens) larvae meal in diets for juvenile Jian carp (Cyprinus carpio var.Jian):growth performance, antioxidant enzyme activities, digestive enzyme activities, intestine and hepatopancreas histological structure[J].Aquaculture, 2017, 477:62-70.
[32] ST-HILAIRE S, SHEPPARD C, TOMBERLIN J, et al.Fly prepupae as a feedstuff for rainbow trout, Oncorhynchus mykiss[J].Journal of the World Aquaculture Society, 38(1):59-67.
[33] HU J R, WANG G X, HUANG Y H, et al.Effects of substitution of fish meal with black soldier fly (Hermetia illucens) larvae meal, in yellow catfish (Pelteobagrus fulvidraco) diets[J].Israeli Journal of Aquaculture-Bamidgeh, 2017, 69:1-9.

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References