AQUATIC ANIMAL NUTRITION AND FEED

Effects of Fish Meal Replacement by Fermented Soybean Meal on Growth Performance, Intestinal Digestive Enzyme Activities, Serum Biochemical Parameters and Intestinal Tissue Structure of Bullfrog

  • CHEN Mingzhe ,
  • CHENG Yunwang ,
  • LU Kangle ,
  • ZHANG Chunxiao ,
  • SONG Kai ,
  • WANG Ling
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  • Xiamen Key Laboratory of Feed Testing and Safety Evaluation, Fisheries College, Jimei University, Xiamen 361021, China

Received date: 2021-04-11

  Online published: 2021-11-10

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Abstract

This experiment was conducted to study the effects of fish meal replacement by fermented soybean meal on growth performance, intestinal digestive enzyme activities, serum biochemical parameters and intestinal tissue structure of bullfrog. A total of 225 healthy bullfrog with initial body weight of (36.0±1.0) g were randomly divided into 5 groups with 3 replicates per group and 15 bullfrog per replicate. Bullfrog in 5 groups were fed experimental diets which use fermented soybean meal replacing 0 (FM group), 25% (FSM25 group), 50% (FSM50 group), 75% (FSM75 group) and 100% (FSM100 group) fish meal, respectively. The experiment lasted for 56 days. The results showed as follows:1) there were insignificant differences in the survival rate among all groups (P>0.05). The weight gain rate of FM group was significantly higher than that of FSM100 group (P<0.05), but there was no significant difference compared with other groups (P>0.05). The feeding rate, feed efficiency and nitrogen retention rate of FM group were no significant difference compared with other groups (P>0.05). 2) There were insignificant differences in contents of moisture, crude protein and crude lipid in whole body and muscle among all groups (P>0.05). The muscle ash content of FSM100 group was significantly lower than that of FSM75 group (P<0.05). 3) The activity of protease in duodenum, jejunum and ileum of FSM25, FSM50, FSM75 and FSM100 groups was significantly lower than that of FM group (P<0.05), and the ileum amylase activity of FSM50, FSM75 and FSM100 groups was significantly higher than that of FM group (P<0.05). 4) The serum catalase (CAT) activity of FSM75 group was significantly higher than that of FM group (P<0.05). The serum malondialdehyde (MDA) content of FSM100 group was significantly lower than that of FM, FSM25 and FSM50 groups (P<0.05). The serum superoxide dismutase (SOD) activity of FSM25 group was significantly higher than that of FM group (P<0.05). The serum glutamic-pyruvic transaminase (GPT) activity of FM group was significantly higher than that of FSM25 group (P<0.05). 5) The histological observation of jejunum showed that when the ratio of fermented soybean meal to fish meal was more than 50%, a large number of basophilic particles appeared locally in the lamina propria, and the villi height was decreased. According to the results of this experiment, it is suggested that the ratio of fermented soybean meal to replace fish meal in bullfrog diet is 50% to 75%.

Cite this article

CHEN Mingzhe , CHENG Yunwang , LU Kangle , ZHANG Chunxiao , SONG Kai , WANG Ling . Effects of Fish Meal Replacement by Fermented Soybean Meal on Growth Performance, Intestinal Digestive Enzyme Activities, Serum Biochemical Parameters and Intestinal Tissue Structure of Bullfrog[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6376 -6386 . DOI: 10.3969/j.issn.1006-267x.2021.11.037

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