Effects of Supplementary Feeding of Guanidine Acetic Acid and Different Methyl Donors on Athletic Performance, Glucose Metabolism and Antioxidative Capacity in Yili Horses

ZHANG Wenjie; LI Xiaobin; LIU Lyu; MA Yan; LU Hao; YANG Hao; OUYANG Wen; YANG Kailun

doi:10.3969/j.issn.1006-267x.2021.11.038

2021 , Vol. 33 >Issue 11: 6387 - 6397

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.11.038

MOLECULAR AND CELLULAR NUTRITION

Effects of Supplementary Feeding of Guanidine Acetic Acid and Different Methyl Donors on Athletic Performance, Glucose Metabolism and Antioxidative Capacity in Yili Horses

ZHANG Wenjie ,
LI Xiaobin ,
LIU Lyu ,
MA Yan ,
LU Hao ,
YANG Hao ,
OUYANG Wen ,
YANG Kailun

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1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, Xinjiang Agricultural University, Urumqi 830052, China;
2. Yili Kazak Autonomous Prefecture of Zhaosu Racecourse, Zhaosu 835000, China

Received date: 2021-04-09

Online published: 2021-11-10

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Abstract

This experiment was conducted to investigate the effects of supplementary feeding of guanidine acetic acid and different methyl donors on athletic performance, glucose metabolism and antioxidant capacity in Yili horses. Twenty well-trained Yili horses aged 2 years, with a body weight of (418.40±39.37) kg and similar race performance at 2 000 m were selected and randomly divided into 4 groups, 5 horses (3♂ +2♀) in each group:control group, guanidine group, guanidine +betaine group and guanidine+methionine group, respectively. Each horse was fed 4 kg concentrate supplement and 8 kg dry forage every day. On this basis, each horse in the guanidine acetic acid group was fed 60 mg/kg BW guanidine acetic acid and 30 mg/kg BW betaine every day in the guanidine acetic acid+betaine group, and each horse in the guanidine acetic acid+methionine group was fed 60 mg/kg BW guanidine acetic acid and 152 mg/kg BW methionine every day. A 25-day trial was conducted. The results showed as follows:1) compared with the control group, supplementation of guanidine acetic acid, guanidine acetic acid+betaine and guanidine acetic acid+methionine could shorten time of the 2 000 m race of Yili horse. The guanidine acetic acid group was better than guanidine acetic acid+betaine group on the average race time reduction and the performance improvement, and significantly higher than the control group and guanidine acetic acid+methionine group (P<0.05). 2) There were no significant differences on heart rate, respiratory rate, guanidine acetic acid, creatine, lactic acid and glucose contents and creatine kinase activity before and after 2 000 m race among guanidine acetic acid group, guanidine acetic acid+betaine group and guanidine acetic acid+methionine group (P>0.05). 3)Compared with the control group, plasma insulin content was significantly increased in the guanidine acetic acid group and guanidine acetic acid+betaine group immediately and 2 h after the race (P<0.05). And plasma glucagon content was significantly increased in the guanidine acetic acid group before and after the race (P<0.05), and it was significantly increased in the guanidine acetic acid+betaine group 2 h before the race and immediately after the race (P<0.05), and the guanidine acetic acid group was superior to the guanidine acetic acid+betaine group. 4) Plasma hydrogen peroxide enzyme activity in guanidine acetic acid group, the guanidine acetic acid+betaine group and guanidine acetic acid+methionine group at race 2 h and immediate were higher than control group (P<0.05), glutathione peroxidase and super oxide dismutase activities and total antioxidant at 2 h before the race were significantly higher than thost in control group (P<0.05), the content of malondialdehyde in the control group were lower, in general, guanidine acetic acid group was better than guanidine acetic acid+betaine group and guanidine acetic acid+methionine group. To sum up, supplemental feeding of guanidine acetic acid and different methyl donors (betaine and methionine) can improve the performance, plasma glucose metabolism and antioxidant capacity in Yili horses of 2 000 m competition performance, and supplemental feeding of 60 mg/kg BW guanidine acetic acid has the best effect.

Key words： Yili horse; guanidine acetic acid; creatine; betaine; methionine

Cite this article

ZHANG Wenjie , LI Xiaobin , LIU Lyu , MA Yan , LU Hao , YANG Hao , OUYANG Wen , YANG Kailun . Effects of Supplementary Feeding of Guanidine Acetic Acid and Different Methyl Donors on Athletic Performance, Glucose Metabolism and Antioxidative Capacity in Yili Horses[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6387 -6397 . DOI: 10.3969/j.issn.1006-267x.2021.11.038

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