MOLECULAR AND CELLULAR NUTRITION

Effects of Vitamin A on Milk Fat Synthesis in Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide

  • HAO Ying ,
  • ZHAO Yanli ,
  • YAN Sumei ,
  • GUO Xiaoyu ,
  • GUO Yongmei ,
  • QI Jingyu
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  • Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-04-01

  Online published: 2021-11-10

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Abstract

The purpose of this experiment was to study the effects of vitamin A on milk fat synthesis in bovine mammary cells (BMECs) induced by hydrogen peroxide (H2O2). This experiment was designed with a single-factor completely randomized trial. The third-generation BMECs were randomly divided into 9 groups with 6 replicates in each group. Among them, the control group (CON group) was cultured with growth medium for 30 h, the H2O2 injury group (H2O2 group) was cultured with CON group medium for 24 h, and then H2O2 was added to continue the culture for 6 h. The seven vitamin A pre-protection groups were cultured for 24 h with different concentrations of vitamin A in the CON group medium, and then added H2O2 for 6 h, the vitamin A concentration was 0.05 (0.05VAH group), 0.10 (0.1VAH group), 0.20 (0.2VAH group), 0.50 (0.5VAH group), 1.00 (1VAH group), 2.00 (2VAH group) and 4.00 μg/mL (4VAH group), respectively. The results showed that compared with the CON group, the H2O2 induced oxidative damage to BMECs, the relative growth rate (RGR), triglyceride (TG) content, total antioxidant capacity (T-AOC) and the activities of total superoxide dismutase (T-SOD), catalase (CAT), glutathione peroxidase (GPx), thioredoxin reductase 1 (TrxR1), fatty acid synthase (FASN), acyl-CoA desaturase (SCD), lipoprotein esterase (LPL) and acetyl-CoA carboxylase (ACC) activities of BMECs in the H2O2 group were significantly reduced (P ≤ 0.05), and the relative expression levels of ACC, FASN, glutathione peroxidase 1 (GPx1), glutathione peroxidase 4 (GPx4), thioredoxin reductase 1 (TrxR1), peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element binding protein 1 (SREBP1) were significantly reduced (P ≤ 0.05), but the activity of reactive oxygen species (ROS) was significantly increased (P ≤ 0.05). Compared with the H2O2 group, the RGR and the relative expression levels of GPx1, GPx4, TrxR1, LPL, SCD, FASN, PPARγ and SREBP1 and the GPx activity, T-AOC of the BMECs in the 0.2VAH to 2VAH groups were significantly increased (P ≤ 0.05), and the TG content, T-SOD and CAT activities of the BMECs in the 1VAH group were significantly increased (P ≤ 0.05), while the ROS activity of the BMECs in the 1VAH group was significantly reduced (P ≤ 0.05). It can be seen that vitamin A has a dose-dependent mitigating effect on the decrease in antioxidant capacity, enzyme activities and gene expressions associated with the de novo synthesis of milk fat and fatty acid uptake in BMECs by H2O2-induced damage. The vitamin A of 0.20 to 2.00 μg/mL has better effect, especially the vitamin A of 1.00 μg/mL has the best effect.

Cite this article

HAO Ying , ZHAO Yanli , YAN Sumei , GUO Xiaoyu , GUO Yongmei , QI Jingyu . Effects of Vitamin A on Milk Fat Synthesis in Bovine Mammary Epithelial Cells Induced by Hydrogen Peroxide[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6398 -6406 . DOI: 10.3969/j.issn.1006-267x.2021.11.039

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