MOLECULAR AND CELLULAR NUTRITION

Effects of Artemisinin on Expression of Milk Fat Synthesis Related Genes in Mammary Epithelial Cells of Dairy Cows

  • HOU Kun ,
  • LI Xin ,
  • SHEN Yiyuan ,
  • ZHAN Jingwei ,
  • NIU Hui ,
  • XIONG Benhai ,
  • TONG Jinjin ,
  • JIANG Linshu
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  • 1. Beijing Key Laboratory of Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Beijing Institute of Animal Husbandry and Veterinary Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-04-17

  Online published: 2021-11-10

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Abstract

This experiment was conducted to investigate the effects of artemisinin on expressions of genes related to milk fat in bovine mammary epithelial cells (BEMCs). The experiment was designed as a single factor randomized trial, cells were cultured in culture medium supplemented with 0 (control), 20, 40, 60, 80 and 100 μmol/L artemisinin for 1, 3, 6, 9, 12 and 24 h, with 6 replicates per group. The results showed that compared with the control group, 20、40 and 60 μmol/L artemisinin could significantly increase the relative activity of BEMCs (P<0.01), significantly increase lipid aggregation and triglyceride (TG) content (P<0.05); real-time fluorescent quantitative PCR results showed that artemisinin could significantly increase milk fat synthesis-related genes peroxisome proliferator-activated receptor γ (PPARγ), sterol regulatory element-binding protein 1-c (SREBP1-c), fatty acid synthase (FASN) and acetyl-CoA carboxylase (ACC) relative expression levels (P<0.05); artemisinin could also significantly increase the relative expression levels of serine/threonine protein kinases 1 (AKT1), mammalian target of rapamycin (mTOR) and stearoyl CoA desaturase 1 (SCD1) genes (P<0.01); Western-blot results showed that 40 μmol/L artemisinin significantly up-regulated the expression of AMPK, mTOR, phosphorylated mammalian target of rapamycin (p-mTOR), PPARγ and SREBP1-c protein (P<0.05). Combined with the above results, the promotion effects of 40 μmol/L artemisinin on improving the milk fat synthesis of BMECs are better, artemisinin promotes the synthesis of milk fat in BMECs by regulating the transcription and translation of key genes in the AMPK-mTOR signaling pathway.

Cite this article

HOU Kun , LI Xin , SHEN Yiyuan , ZHAN Jingwei , NIU Hui , XIONG Benhai , TONG Jinjin , JIANG Linshu . Effects of Artemisinin on Expression of Milk Fat Synthesis Related Genes in Mammary Epithelial Cells of Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6407 -6419 . DOI: 10.3969/j.issn.1006-267x.2021.11.040

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