MOLECULAR AND CELLULAR NUTRITION

Effects of Artemisia annua L. Extracts on Rumen Fermentation Parameters and Microflora of Lactating Dairy Cows

  • YU Shiqiang ,
  • XIONG Anran ,
  • PAN Yucong ,
  • WANG Yue ,
  • ZHANG Yajing ,
  • JIANG Linshu ,
  • XIONG Benhai
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  • 1. Key Laboratory for Dairy Cow Nutrition, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
    2. Beijing Institute of Animal Science and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Hebei Gaobeidian Vocational Education Center, Baoding 074000, China

Received date: 2021-04-01

  Online published: 2021-11-10

Supported by

 

Abstract

The experiment was conducted to investigate the effects of Artemisia annua L. extract on rumen fermentation parameters and microflora of Holstein cows. Twenty Chinese Holstein cows in mid-lactation with similar body condition, two to four fetuses and a daily milk production of (33.0±3.9) kg/d were selected and randomly divided into four groups, with five replicates in each group. The control group was fed a basic diet, and the experimental group was added 0.25%, 0.50% and 0.75% of Artemisia annua L. extract in the basic diet, respectively. The pre-trial period was 10 d and the positive trial period was 35 d. The results showed as follows:1) compared with the control group, when 0.25% and 0.50% Artemisia annua L. extract was added, the concentration of propionic acid was significantly increased (P<0.05), and the ratio of ethylene to propylene was significantly decreased (P<0.05). When 0.75% Artemisia annua L. extract was added, the total volatile acid, propionic acid, butyric acid and valeric acid concentrations were significantly increased (P<0.05), and the acetic acid, isobutyric acid concentrations and ethylene-propylene ratio were significantly decreased (P<0.05). 2) Ace index and Chao1 index in experimental groups were significantly higher than those in the control group (P<0.05), and there was no significant difference in Shannon index and Simpson index (P>0.05). 3) At the phylum level, compared with the control group, the relative abundance levels of Firmicutes and Proteobacyeria were significantly increased (P<0.05) in the experimental groups, while the relative abundance levels of Bacteroidetes, SR_[Absconditabacteria] and Verrucomicrobia were significantly decreased (P<0.05) in the experimental groups. At the genus level, compared with the control group, the relative abundance levels of Prevotella 1, Rikenellaceae_RC9_gut_group and Succiniclasticum were significantly increased (P<0.05) in the experimental groups, while the relative abundance levels of Butyrivibrio 2, Ruminococcaceae_NK4A214_group and Ruminococcus 2 were significantly decreased (P>0.05). 4) At the genus level, the relative abundance level of Succinivibrionaceae_UCG-002 had a very significant positive correlation with acetic acid, total volatile acid and propionic acid concentrations (P<0.01, R>0.500); the relative abundance level of Ruminococcaceae_UCG-014 was significantly and negatively correlated with butyric acid concentration (P<0.01, R=-0.415); the relative abundance level of Ruminococcus 1 had a significantly negative correlation with acetic acid concentration (P<0.05, R=-0.433); the relative abundance level of Prevotella 1 was significantly and negatively correlated with acetic acid, total volatile acid and ammoniacal nitrogen concentrations (P<0.01, R>0.500). The relative abundance level of Prevotellaceae_UCG-001 had a very significant negative correlation with acetic acid concentration (P<0.01, R=-0.789); the relative abundance levels of Rikenellaceae_RC9_gut_group and acetic acid concentration had a very significant negative correlation (P<0.01, R>0.500). Adding 0.75% Artemisia annua L. extract can change the abundance of rumen bacteria, reduce the concentration of acetic acid and ethylene-propylene ratio in the rumen, increase the concentration of total volatile acid, propionic acid, butyric acid and valeric acid, and improve the rumen fermentation function.

Cite this article

YU Shiqiang , XIONG Anran , PAN Yucong , WANG Yue , ZHANG Yajing , JIANG Linshu , XIONG Benhai . Effects of Artemisia annua L. Extracts on Rumen Fermentation Parameters and Microflora of Lactating Dairy Cows[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6431 -6444 . DOI: 10.3969/j.issn.1006-267x.2021.11.042

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