EXPERIMENTAL METHOD AND ANIMAL

Comparison of Effects of Enterococcus faecium with Different Activities on Rumen in Vitro Fermentation in Sheep Using in Vitro Gas Production Method

  • BAI Tiantian ,
  • CUI Haoran ,
  • JIANG Chenyu ,
  • ZHOU Xiaoling ,
  • GUO Xuefeng
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  • 1. College of Animal Science, Tarim University, Alar 843300, China;
    2. Key Laboratory of Tarim Animal Husbandry Science and Technology, Tarim University, Alar 843300, China

Received date: 2021-04-01

  Online published: 2021-11-10

Supported by

 

Abstract

This experiment was aimed to study the effects of adding Enterococcus faecium with different activities on the in vitro fermentation parameters of the rumen of sheep, and explore its optimal addition level in vivo. In this experiment, the control group, test group 1, test group 2 and test group 3 were set up by the in vitro gas production method. The activities of Enterococcus faecium were 0, 1×1012, 1×1011 and 1×1010 CFU/mL, respectively. They were cultured for 3, 6, 12, 24 and 48 h to measure gas production, and pH, dry matter degradation rate, ammonia nitrogen (NH3-N) and volatile fatty acids (VFA) concentrations were measured at 24 and 48 h. The results showed that the gas production of each group was significantly higher than that of the control group (P<0.05); the pH of each group showed a downward trend with the increase of fermentation time; the dry matter degradation rate gradually increased with fermentation time increase, and the dry matter degradation rate of test group 3 was significantly higher than that of the control group at fermentation 24 h (P<0.05); the NH3-N concentration of each group increased with the decrease of activity; at fermentation 24 h, the NH3-N concentration of test group 3 was significantly higher than that of the control group (P<0.05); adding Enterococcus faecium significantly increased the concentrations of acetic acid, propionic acid and total VFA (P<0.05). To sum up, through the analysis and comparison of various indicators, the optimal added activity of Enterococcus faecium is 1×1010 CFU/mL.

Cite this article

BAI Tiantian , CUI Haoran , JIANG Chenyu , ZHOU Xiaoling , GUO Xuefeng . Comparison of Effects of Enterococcus faecium with Different Activities on Rumen in Vitro Fermentation in Sheep Using in Vitro Gas Production Method[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6483 -6491 . DOI: 10.3969/j.issn.1006-267x.2021.11.047

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