EXPERIMENTAL METHOD AND ANIMAL

Effects of Linseed Oil on Rumen Methane, Hydrogen Production and Fatty Acid Composition Using in Vitro Method

  • TAN Jian ,
  • WANG Rong ,
  • ZHANG Xiumin ,
  • MA Zhiyuan ,
  • WANG Min ,
  • TAN Zhiliang
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  • 1. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    2. College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-04-06

  Online published: 2021-11-10

Supported by

 

Abstract

In this study, the mechanism of linseed oil on rumen methane production was studied from the perspective of rumen hydrogen metabolism. The single factor experimental design was used to evaluate the effects of supplemental levels of linseed oil[0 (control), 1%, 2%, 3%, 4% and 5%] on gas production, hydrogen and methane production and molar ratio of fatty acids after 48 h of rumen fermentation using the automatic fermentation incubation system. Rumen fluid was collected from three Xiangdong black goats with permanent rumen fistulas. Each group had 2 replicates, and were replicated 3 times. The results showed that the gas production, dry matter degradation, methane and hydrogen production, molar ratios of myristic acid, palmitic acid, palmitic oleic acid, heptadecanoic acid and saturated fatty acids in linseed oil groups were linearly decreased while the molar ratios of cis-oleic acid, linolenic acid and unsaturated fatty acids were linearly increased with the increasing of supplemental levels of linseed oil, and the effects of 4% and 5% linseed oil were significant (P<0.05). The unsaturated fatty acids in linseed oil enhance the hydrogen utilization, leading to decrease hydrogen production, meanwhile decrease the feed degradation and methane production.

Cite this article

TAN Jian , WANG Rong , ZHANG Xiumin , MA Zhiyuan , WANG Min , TAN Zhiliang . Effects of Linseed Oil on Rumen Methane, Hydrogen Production and Fatty Acid Composition Using in Vitro Method[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6492 -6500 . DOI: 10.3969/j.issn.1006-267x.2021.11.048

References

[1] TORRES M G, SORISNO R, PERALTA J G, et al.Challenges of livestock:climate change, animal welfare and agroforestry[J].Large Animal Review, 2020, 26(1):39-45.
[2] FELDMAN D R, COLLINS W D, BIRAUD S C, et al.Observationally derived rise in methane surface forcing mediated by water vapour trends[J].Nature Geoscience, 2018, 11:238-243.
[3] 程胜利, 肖玉萍, 杨保平, 等.反刍动物甲烷排放现状及调控技术研究进展[J].中国草食动物科学, 2013, 33(5):56-59. CHENG S L, XIAO Y P, YANG B P, et al.Research progress in methane emission from ruminant and control techniques[J].China Herbivore Science, 2013, 33(5):56-59.(in Chinese)
[4] JOHNSON K A, JOHNSON D E.Methane emissions from cattle[J].Journal of Animal Science, 1995, 73(8):2483-2492.  
[5] MIN B R, SOLAIMAN S, WALDRIP H M, et al.Dietary mitigation of enteric methane emissions from ruminants:a review of plant tannin mitigation options[J].Animal Nutrition, 2020, 6(3):231-246.  
[6] 金舒文, 王佳堃.瘤胃产甲烷菌与其他微生物间的氢传递及其调控研究进展[J].中国畜牧杂志, 2019, 55(2):1-6. JIN S W, WANG J K.Interspecific hydrogen transfer between methanogens and other microorganisms in rumen and its regulation strategies:a review[J].Chinese Journal of Animal Science, 2019, 55(2):1-6.(in Chinese)
[7] ZHANG X M, MEDRANO R F, WANG M, et al. Effects of urea plus nitrate pretreated rice straw and corn oil supplementation on fiber digestibility, nitrogen balance, rumen fermentation, microbiota and methane emissions in goats[J].Journal of Animal Science and Biotechnology, 2019, 10:6.
[8] WANG R, WANG M, UNGERFELD E M, et al.Nitrate improves ammonia incorporation into rumen microbial protein in lactating dairy cows fed a low-protein diet[J].Journal of Dairy Science, 2018, 101(11):9789-9799.  
[9] KANDI M, KAZEMI-BONCHENARI M, HOSSEINYAZDI M, et al.Effects of Ca-salt of linseed oil supplementation and protein content in diet on performance, ruminal fermentation, microbial protein yield, and blood metabolites in young lambs[J].Small Ruminant Research, 2020, 193:106257.
[10] MAJEWSKA M P, MILTKO R, KRAWCZY Ń SKA A, et al.Rapeseed and linseed oil supplementation affects hydrolytic activities in the rumen of sheep[J].Livestock Science, 2020, 240:104175.
[11] MOALLEM U, LEHRER H, LIVSHITS L, et al.The effects of omega-3α-linolenic acid from flaxseed oil supplemented to high-yielding dairy cows on production, health, and fertility[J].Livestock Science, 2020, 242:104302.
[12] LYONS T, BOLAND T, STOREY S, et al.Linseed oil supplementation of lambs' diet in early life leads to persistent changes in rumen microbiome structure[J].Frontiers in Microbiology, 2017, 8:1656.
[13] WANG X E, MARTIN G B, WEN Q, et al.Linseed oil and heated linseed grain supplements have different effects on rumen bacterial community structures and fatty acid profiles in cashmere kids[J].Journal of Animal Science, 2019, 97(5):2099-2113.  
[14] DEWANCKELE L, TORAL P G, VLAEMINCK B, et al.Invited review:role of rumen biohydrogenation intermediates and rumen microbes in diet-induced milk fat depression:an update[J].Journal of Dairy Science, 2020, 103(9):7655-7681.  
[15] MAIA M R G, CHAUDHARY L C, FIGUERES L, et al.Metabolism of polyunsaturated fatty acids and their toxicity to the microflora of the rumen[J].Antonie Van Leeuwenhoek, 2007, 91:303-314.
[16] CONE J W, BECKER P M.Fermentation kinetics and production of volatile fatty acids and microbial protein by starchy feedstuffs[J].Animal Feed Science and Technology, 2012, 172(1/2):34-41.
[17] WANG M, WANG R, YANG S, et al.Effects of three methane mitigation agents on parameters of kinetics of total and hydrogen gas production, ruminal fermentation and hydrogen balance using in vitro technique[J].Animal Science Journal, 2016, 87(2):224-232.  
[18] WANG R, SI H B, WANG M, et al.Effects of elemental magnesium and magnesium oxide on hydrogen, methane and volatile fatty acids production in in vitro rumen batch cultures[J].Animal Feed Science and Technology, 2019, 252:74-82.
[19] WANG M, JANSSEN P H, SUN X Z, et al.A mathematical model to describe in vitro kinetics of H2 gas accumulation[J].Animal Feed Science and Technology, 2013, 184(1/2/3/4):1-16.
[20] WANG M, SUN X Z, JANSSEN P H, et al.Responses of methane production and fermentation pathways to the increased dissolved hydrogen concentration generated by eight substrates in in vitro ruminal cultures[J].Animal Feed Science and Technology, 2014, 194:1-11.
[21] ZHANG X M, MEDRANO R F, WANG M, et al.Corn oil supplementation enhances hydrogen use for biohydrogenation, inhibits methanogenesis, and alters fermentation pathways and the microbial community in the rumen of goats[J].Journal of Animal Science, 2019, 97(12):4999-5008.  
[22] ZHANG X M, WANG M, WANG R, et al.Urea plus nitrate pretreatment of rice and wheat straws enhances degradation and reduces methane production in in vitro ruminal culture[J].Journal of the Science of Food and Agriculture, 2018, 98(14):5205-5211.  
[23] ZHANG X M, WANG M, YU Q, et al.Liquid hot water treatment of rice straw enhances anaerobic degradation and inhibits methane production during in vitro ruminal fermentation[J].Journal of Dairy science, 2020, 103(5):4252-4261.  
[24] MAJEWSKA M P, MILTKO R, BELZECKI G, et al.Supplementation of rapeseed and linseed oils to sheep rations:effects on ruminal fermentation characteristics and protozoal populations[J].Czech Journal of Animal Science, 2017, 62(12):527-538.  
[25] MARTIN C, ROUEL J, JOUANY J P, et al.Methane output and diet digestibility in response to feeding dairy cows crude linseed, extruded linseed, or linseed oil[J].Journal of Animal Science, 2008, 86(10):2642-2650.  
[26] MARTIN C, FERLAY A, MOSONI P, et al.Increasing linseed supply in dairy cow diets based on hay or corn silage:effect on enteric methane emission, rumen microbial fermentation, and digestion[J].Journal of Dairy Science, 2016, 99(5):3445-3456.  
[27] MARTIN C, MORGAVI D P, DOREAU M.Methane mitigation in ruminants:from microbe to the farm scale[J].Animal, 2010, 4(3):351-365.  
[28] SZUMACHER-STRABEL M, MARTIN S A, POTKA Ń SKI A, et al.Changes in fermentation processes as the effect of vegetable oil supplementation in in vitro studies[J].Journal of Animal and Feed Sciences, 2004, 13(S1):215-218.
[29] 刘国防, 梁志伟, 杨尚源, 等.油脂废水生物处理研究进展[J].应用生态学报, 2011, 22(8):2219-2226. LIU G F, LIANG Z W, YANG S Y, et al.Bio-treatment of grease wastewater:research prowess[J].Chinese Journal of Applied Ecology, 2011, 22(8):2219-2226.(in Chinese)
[30] VAN ZIJDERVELD S M, GERRITS W J J, APAJALAHTI J A, et al.Nitrate and sulfate:effective alternative hydrogen sinks for mitigation of ruminal methane production in sheep[J].Journal of Dairy Science, 2010, 93(12):5856-5866.  
[31] JUDY J V, BACHMAN G C, BROWN-BRANDL T M, et al.Increasing the concentration of linolenic acid in diets fed to Jersey cows in late lactation does not affect methane production[J].Journal of Dairy Science, 2019, 102(3):2085-2093.  
[32] BOLAND T M, PIERCE K M, KELLY A K, et al.Feed intake, methane emissions, milk production and rumen methanogen populations of grazing dairy cows supplemented with various C 18 fatty acid sources[J].Animals, 2020, 10(12):2380.
[33] MARTIN C, COPPA M, FOUGōRE H, et al.Diets supplemented with corn oil and wheat starch, marine algae, or hydrogenated palm oil modulate methane emissions similarly in dairy goats and cows, but not feeding behavior[J].Animal Feed Science and Technology, 2021, 272:114783.
[34] MORGAVI D P, MARTIN C, JOUANY J P, et al.Rumen protozoa and methanogenesis:not a simple cause-effect relationship[J].The British Journal of Nutrition, 2012, 107(3):388-397.  
[35] SATO Y, TOMINAGA K, AOKI H, et al.Calcium salts of long-chain fatty acids from linseed oil decrease methane production by altering the rumen microbiome in vitro[J].PLoS One, 2020, 15(11):e0242158.
[36] 黄国欣, 张养东, 郑楠, 等.牛乳中ω-3多不饱和脂肪酸调控的研究进展[J].动物营养学报, 2019, 31(11):4918-4927. HUANG G X, ZHANG Y D, ZHENG N, et al.Research advances in regulation of ω-3 polyunsaturated fatty acids in milk[J].Chinese Journal of Animal Nutrition, 2019, 31(11):4918-4927.(in Chinese)
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