Structure and Composition of Rumen Bacteria in Shaanbei White Cashmere Goats with Fine and Coarse

LI Longping; LIU Jinwang; LI Tuo; ZHU Haijing; QU Lei

doi:10.3969/j.issn.1006-267x.2021.11.050

Chinese Journal of Animal Nutrition >

2021 , Vol. 33 >Issue 11: 6510 - 6522

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.11.050

SHORT COMMUNICATION

Structure and Composition of Rumen Bacteria in Shaanbei White Cashmere Goats with Fine and Coarse

LI Longping ,
LIU Jinwang ,
LI Tuo ,
ZHU Haijing ,
QU Lei

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1. Shaanxi Province Engineering & Technology Research Center of Cashmere Goat, Yulin University, Yulin 719000, China;
2. Yulin Sannong Breeding Service Co., Ltd., Yulin 719000, China;
3. College of Life Sciences, Yulin University, Yulin 719000, China

Received date: 2021-04-25

Online published: 2021-11-10

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Abstract

The purpose of this study was to use 16S rRNA high-throughput sequencing technology to analyze the structure and composition of rumen bacteria in coarse and fine cashmere goats, and provide a basis for the breeding of fine cashmere goats. Thirteen 2-year-old Shaanbei white cashmere female goats (6 fine cashmere goats and 7 coarse cashmere goats) were selected under the same feeding conditions. Rumen fluid was collected, and total DNA was extracted. After PCR amplification, the V3 to V4 region of 16S rDNA were sequenced by high-throughput sequencing technology. The results showed as follows:1) the differences of the Faith's PD index, Chao1 index, Shannon index and Observed_OTU index between two groups were not significant (P>0.05), and all those indexes showed that the fine cashmere group were lower than the coarse cashmere group. 2) At phylum level, the dominant bacterial phyla of rumen bacteria in two groups were Firmicutes and Bacteroidetes, followed by Actinobacteria, Cyanobacteria, Proteobacteria, Synergistetes, Spirochaetes, Tenericutes and Verrucomicrobia; the dominant bacterial genera of rumen bacteria in two groups were Ruminococcus, Prevotella, Unspecified_Bacteroidales and Unspecified_Ruminococcaceae. 3) For the dominant bacterial phylum (top 10 of relative abundance), the relative abundance of Proteobacteria in the coarse cashmere group was significantly higher than that of the fine cashmere group (P<0.05), while the relative abundance of TM7 in the coarse cashmere was significantly lower than that of the fine cashmere group (P<0.05), and the relative abundances of other phylum between two groups were not significant (P>0.05). For the sub-dominant phylum, the relative abundances of Elusimicrobia, Lentisphaerae and Verrucomicrobia in the coarse cashmere group were significantly higher than those of the fine cashmere group (P<0.05), while the relative abundance of WPS_2 in the coarse cashmere group was significantly lower than that of the fine cashmere group (P<0.05). 4) For the dominant bacterial genera (relative abundance>10%), except that the relative abundance of Prevotella in the fine cashmere group was extremely significantly higher than that of the coarse cashmere group (P<0.01), there was no significant difference between the two groups of the relative abundances of other dominant bacterial genera (P>0.05). For sub-dominant bacterial genera (relative abundance<10%), the relative abundances of Succiniclasticum, Oscillospira, Dehalobacterium, Blautia, Clostridium and Erwinia in the coarse cashmere group were significantly higher than those in the fine cashmere group (P<0.05). 5) Species correlation network analysis indicated that the correlation and compactness of the rumen bacteria in fine cashmere group were higher than coarse cashmere group. In conclusion, the rumen microflora number and relative abundance of fine cashmere goats are lower than those of the coarse cashmere goats. There exist some characteristic rumen bacteria in the fine cashmere and coarse cashmere groups. The interaction and structural compactness of rumen microflora of the fine cashmere goats are higher than those of the coarse cashmere goats, suggesting that the interaction and stability of rumen microflora may affect the cashmere quality.

Key words： fine cashmere; coarse cashmere; Shaanbei white cashmere goats; rumen bacteria; high throughput sequencing

Cite this article

LI Longping , LIU Jinwang , LI Tuo , ZHU Haijing , QU Lei . Structure and Composition of Rumen Bacteria in Shaanbei White Cashmere Goats with Fine and Coarse[J]. Chinese Journal of Animal Nutrition, 2021 , 33(11) : 6510 -6522 . DOI: 10.3969/j.issn.1006-267x.2021.11.050

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