Effects and Mechanism Research of Soybean Isoflavones on Intestinal Epithelial Cells of Piglets under Oxidative Stress in Vitro

HU Shenglan; XIAO Hao; WANG Li; HUANG Ling; JIANG Zongyong

doi:10.3969/j.issn.1006-267x.2021.12.012

2021 , Vol. 33 >Issue 12: 6699 - 6708

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.12.012

SWINE NUTRITION AND FEED

Effects and Mechanism Research of Soybean Isoflavones on Intestinal Epithelial Cells of Piglets under Oxidative Stress in Vitro

HU Shenglan ,
XIAO Hao ,
WANG Li ,
HUANG Ling ,
JIANG Zongyong

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Guangdong Key Laboratory of Animal Breeding and Nutrition, Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2021-05-13

Online published: 2021-12-16

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Abstract

This experiment was conducted to investigate the effects of soybean isoflavones (SI) on the antioxidant capacity and immune performance of intestinal epithelial cells of piglets under oxidative stress. Based on the oxidative stress model of piglets' intestinal epithelial cells established by FeSO₄/H₂O₂, the antioxidant enzyme activities, immunological cytokine secretion and related signaling pathways were measured after 48 h treatment with different concentrations of SI. A normal control group and an oxidized control group were set at the same time. The results showed that, compared with the oxidized control group, the addition of 10 to 40 μmol/L SI significantly increased the cell proliferation rate (P<0.05), and significantly decreased the cell apoptosis rate (P<0.05). Compared with the normal control group, the contents of interleukin-1β (IL-1β), interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) were significantly increased (P<0.05), and the content of interleukin-4 (IL-4) was significantly decreased by oxidation treatment (P<0.05). The addition of 10 to 80 μmol/L SI groups had a significantly lower TNF-α content than that of the oxidized control group (P<0.05). In addition, oxidation treatment increased the content of nuclear factor-κB (NF-κB) and the activity of Jun N-terminal kinase (JNK), and the addition of 10 to 20 μmol/L SI inhibited the protein expression of nitric oxide (NO), inducible nitric oxide synthase (iNOS), NF-κB and JNK in cells. In conclusion, the addition of SI can improve the antioxidant capacity of intestinal epithelial cells of piglets under oxidative stress in vitro, and inhibit the inflammatory response induced by oxidative stress by regulating NF-κB and JNK signaling pathways.

Key words： soybean isoflavones; intestinal epithelial cells of piglets; antioxidant; inflammatory response; signal pathway

Cite this article

HU Shenglan , XIAO Hao , WANG Li , HUANG Ling , JIANG Zongyong . Effects and Mechanism Research of Soybean Isoflavones on Intestinal Epithelial Cells of Piglets under Oxidative Stress in Vitro[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6699 -6708 . DOI: 10.3969/j.issn.1006-267x.2021.12.012

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