RUMINANT AND HERBIVORE NUTRITION AND FEED

Effects of Dietary Crude Protein Levels on Respiration Metabolism, Nutrient Apparent Digestibility and Serum Biochemical Indices of Steppe Red Cattle

  • ZHANG Xianglun ,
  • LIU Jiwei ,
  • ZHAO Hongbo ,
  • WANG Lei ,
  • LIU Xiaomu ,
  • YOU Wei ,
  • QIN Lihong ,
  • ZHAO Yumin ,
  • WAN Fachun ,
  • ZHANG Guoliang
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  • 1. Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Ji'nan 250100, China;
    2. Institute of Animal Husbandry, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, China;
    3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

Received date: 2021-05-16

  Online published: 2021-12-16

Abstract

This study was carried out to investigate the effects of dietary crude protein levels on respiration metabolism, nutrient apparent digestibility and serum biochemical indices of Steppe Red cattle and to provide reference for feeding standard formulation of Steppe Red cattle. A total of twelve healthy Steppe Red cattle with similar body weight of (473.08±14.20) kg were randomly allocated into three groups with four cattle each. Cattle was fed diets with crude protein level of 8.00% (low-protein group), 10.00% (middle-protein group) and 12.00% (high-protein group), respectively. The experiment lasted for 20 days, including a pre-trial period for 18 days and a formal trial period for 2 days. During the formal trial period, an equipment of macrofauna open-reflux type with respiration and calorimetric function was used to carry out the respiratory calorimetry test, and the digestion and metabolism test was carried out by the total fecal and urine collection method. At the end of the trial, blood samples were collected from jugular vein of cattle to isolate serum for measurement. The results showed as follows:1) the methane production, oxygen consumption, carbon dioxide production and heat production of Steppe Red cattle in low-protein group were significantly higher than those in the other two groups (P<0.05), and all the indices above were increased linearly with the increase of dietary crude protein level (P<0.05). 2) The urinary nitrogen excretion of Steppe Red cattle in high-protein group was significantly higher than that in the other two groups (P<0.05), while the digested nitrogen and deposited nitrogen in low-protein group were significantly lower than those in the other two groups (P<0.05), and all the indices above were increased linearly with the increase of dietary crude protein level (P<0.05). Dietary crude protein levels did not significantly affect the apparent digestibility and apparent metabolic rate of nitrogen of Steppe Red cattle (P>0.05). 3) The urinary energy excretion of Steppe Red cattle tended to be increased linearly with the increase of dietary crude protein level (P=0.056). The methane energy emission and the ratio of methane energy emission to gross energy of Steppe Red cattle in low-protein group were significantly lower than those in the other two groups (P<0.05), and the two indices were decreased linearly with the increase of dietary crude protein level (P<0.05). The other energy metabolism indices did not significantly differ among groups (P>0.05). 4) The nutrient apparent digestibility was not significantly affected by dietary crude protein levels (P>0.05). 5) The serum urea nitrogen content of Steppe Red cattle in high-protein group was significantly higher than that in the other two groups (P<0.05), and it was increased linearly with the increase of dietary crude protein level (P<0.05). The other serum biochemical indices did not significantly differ among groups (P>0.05). Overall, the effect of middle-protein group was the best, and the recommended dietary crude protein level for 450 kg Steppe Red cattle is 10.00%. The daily supply quantity of digested nitrogen and digestible energy was 110.61 g/d and 102.21 MJ/d, and that of deposited nitrogen and metabolic energy was 98.17 g/d and 92.67 MJ/d, respectively.

Cite this article

ZHANG Xianglun , LIU Jiwei , ZHAO Hongbo , WANG Lei , LIU Xiaomu , YOU Wei , QIN Lihong , ZHAO Yumin , WAN Fachun , ZHANG Guoliang . Effects of Dietary Crude Protein Levels on Respiration Metabolism, Nutrient Apparent Digestibility and Serum Biochemical Indices of Steppe Red Cattle[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6833 -6842 . DOI: 10.3969/j.issn.1006-267x.2021.12.025

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