Effects of Palygorskite Supplementation on Growth Performance, Diarrhea Incidence, Serum Biochemical Indexes and Intestinal Microflora of Holstein Calves

WANG Dong; DU Yuanyi; ZHENG Duo; YOU Zhendong; LIU Yun

doi:10.3969/j.issn.1006-267x.2021.12.026

2021 , Vol. 33 >Issue 12: 6843 - 6852

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.12.026

RUMINANT AND HERBIVORE NUTRITION AND FEED

Effects of Palygorskite Supplementation on Growth Performance, Diarrhea Incidence, Serum Biochemical Indexes and Intestinal Microflora of Holstein Calves

WANG Dong ,
DU Yuanyi ,
ZHENG Duo ,
YOU Zhendong ,
LIU Yun

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Heilongjiang Key Laboratory of Experimental Animals and Comparative Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China

Received date: 2021-04-27

Online published: 2021-12-16

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Abstract

This experiment was conducted to study the effects of palygorskite (PAL) supplementation on the growth performance, diarrhea incidence, serum biochemical indexes and intestinal microflora of Holstein calves. Thirty newborn calves with similar body weight were randomly assigned 2 groups with 15 replicates per group and 1 calve per replicate. Calves in control group were fed a basal diet, while those in PAL group were fed the basal diet+2 000 mg/kg PAL. The experiment lasted for 63 days. The results showed as follows:1) compared with the control group, PAL supplementation significantly increased the average daily gain (ADG) of calves from 1 to 21 days of age (P<0.05), and significantly increased the average daily feed intake (ADFI) of calves from 1 to 21 days of age and 57 to 63 days of age (P<0.05). 2) Compared with the control group, calves in the PAL group had significantly lower diarrhea incidence and fecal score from 1 to 21 days of age and 57 to 63 days of age (P<0.05). 3) The concentrations of immunoglobulin A (IgA), immunoglobulin M (IgM) in serum of calves at 21 days of age, the concentration of immunoglobulin G (IgG) in serum of calves at 56 days of age, and the concentration of IgA in serum of calves at 63 days of age in the PAL group were significantly higher than those in the control group (P<0.05). 4) Compared with the control group, calves in the PAL group had significantly higher serum interleukin-4 (IL-4) concentration of calves at 21 days of age and serum glutathione peroxidase (GSH-Px) activity and total antioxidant capacity (T-AOC) of calves at 63 days of age (P<0.05), but had significantly lower serum interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and malondialdehyde (MDA) concentrations of calves at 63 days of age (P<0.05). 5) Compared with the control group, the counts of intestinal total bacterial of calves at 21 days of age and Bifidobacterium and Bacillus of calves at 63 days of age in the PAL group were significantly increased (P<0.05), while the count of intestinal E.coli of calves at 21 and 63 days of age was significantly decreased (P<0.05). In summary, under this experimental condition, PLA supplementation can improve the ADG (1 to 21 days of age), enhance the immune status and antioxidant capacity, modify the intestinal microflora and reduce the diarrhea incidence of Holstein calves.

Key words： palygorskite; Holstein calves; diarrhea incidence; serum biochemical indexes; intestinal microflora

Cite this article

WANG Dong , DU Yuanyi , ZHENG Duo , YOU Zhendong , LIU Yun . Effects of Palygorskite Supplementation on Growth Performance, Diarrhea Incidence, Serum Biochemical Indexes and Intestinal Microflora of Holstein Calves[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6843 -6852 . DOI: 10.3969/j.issn.1006-267x.2021.12.026

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