RUMINANT AND HERBIVORE NUTRITION AND FEED

Effects of Guanidine Acetic Acid on Growth Performance, Plasma Antioxidant and Glycometabolism Indexes and Blood Related Genes Expression of Beef Cattle

  • WANG Ziyuan ,
  • CHEN Guangji ,
  • SHU Jianhong ,
  • ZHANG Rong ,
  • MA Guangzhi ,
  • HE Tingzhang ,
  • WANG Xiaoli
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  • 1. Guizhou Institute of Prataculture, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;
    2. Anhui Wanhejiaer Biological Technology Co., Ltd., Chuzhou 239000, China;
    3. Agricultural Service Center of Jianba Town of Suiyang County, Suiyang 563307, China

Received date: 2021-04-28

  Online published: 2021-12-16

Abstract

This experiment was conducted to investigate the effects of guanidine acetic acid on growth performance, plasma antioxidant and glycometabolism indexes and blood related genes expression of beef cattle. Twenty-four healthy Simmental beef cattle with an average weight of (473.58±64.40) kg were selected and randomly divided into three groups with eight replicates per group and one cattle per replicate. The cattle in the control group were fed a basal diet, and the others in experimental groups were fed the basal diets supplemented with 400 (G1 group) and 800 mg/kg (G2 group) guanidine acetic acid, respectively. The pretest lasted for 7 days,and the experimental period lasted for 49 days. The results showed as follows:1) compared with the control group, the average daily gain of G2 group was significantly increased (P<0.05), and the feed to gain ratio was significantly decreased (P<0.05). There were no significant differences in final weight and average daily feed intake among all groups (P>0.05). 2) Compared with the control group, the total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) activity in plasma of G1 group and G2 group were significantly increased (P<0.05), the plasma catalase (CAT) activity was significantly increased (P<0.01), and the plasma malondialdehyde (MDA) content was significantly decreased (P<0.01). There was no significant difference in plasma superoxide dismutase (SOD) activity among all groups (P>0.05). 3) Compared with the control group, the plasma glucose-6-phosphate dehydrogenase (G-6-PDH) activity of G1 group and G2 group was significantly decreased (P<0.01), and the plasma pyruvate kinase (PK) activity was significantly decreased (P<0.05); the plasma phosphofructokinase (PFK) activity of G2 group was significantly decreased (P<0.05); the plasma creatine kinase (CK) activity of G1 group and G2 group was significantly increased (P<0.05). There was no significant difference in plasma creatinine (CRE) content among all groups (P>0.05). 4) Compared with the control group, the mRNA relative expression levels of creatine transporter (CrT), adenylate activated protein kinase (AMPK) and γ-aminobutyric acid transporter 2 (GAT2) in blood of G1 group and G2 group were significantly decreased (P<0.01). In summary, the addition of guanidine acetic acid in diets can down-regulate the mRNA expression of CrT, AMPK and GAT2 in blood, reduce catabolism of carbohydrate, and enhance the body antioxidant capacity, thereby improve the growth performance of beef cattle.

Cite this article

WANG Ziyuan , CHEN Guangji , SHU Jianhong , ZHANG Rong , MA Guangzhi , HE Tingzhang , WANG Xiaoli . Effects of Guanidine Acetic Acid on Growth Performance, Plasma Antioxidant and Glycometabolism Indexes and Blood Related Genes Expression of Beef Cattle[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6853 -6863 . DOI: 10.3969/j.issn.1006-267x.2021.12.027

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