RUMINANT AND HERBIVORE NUTRITION AND FEED

Analysis of Plasma Metabolomics of Tan Sheep with Different Residual Feed Intake

  • LU Tongtong ,
  • WANG Junkui ,
  • LUO Fang ,
  • HE Dongqian ,
  • CHEN Liyao ,
  • ZHANG Qian ,
  • WU Shaofei ,
  • GUO Yansheng ,
  • ZHANG Ruixue ,
  • TAO Jinzhong
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  • College of Agriculture, Ningxia University, Yinchuan 750021, China

Received date: 2021-04-27

  Online published: 2021-12-16

Abstract

The purpose of this experiment was to explore the differences of plasma metabolites among different residual feed intake (RFI) Tan sheep and to screen candidate biomarkers. In this experiment, 13 plasma samples of male Tan sheep and 13 plasma samples of female Tan sheep were collected, a total of 26 plasma samples were collected[including 7 plasma samples of extreme high RFI rams (EHM) and 7 plasma samples of extreme high RFI ewes (EHF), 6 plasma samples of extreme low RFI rams (ELM) and 6 plasma samples of extreme low RFI ewes (ELF)]. Ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabonomic technique was used to screen the differential metabolites in different groups of Tan sheep plasma, combined with principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). The results showed that five differential metabolites were down-regulated in the ELM vs EHM group; two differential metabolites were down-regulated in the ELF vs EHF group; four differential metabolites were down-regulated in the EL vs EH group. The receiver operating characteristic curve (ROC curve) and box diagram were used to further detect the ability of these differential metabolites to identify the groups, and the results showed that 3-hydroxy-3-methyl butyric acid, carnitine C9:0, carnitine C7:0 and carnitine C9:0 isomer could distinguish RFI significantly in male Tan sheep. Carnitine C18:2 could distinguish RFI significantly in female Tan sheep. In conclusion, plasma differential metabolites are mainly involved in lipid metabolism, among which 3-hydroxy-3-methyl butyric acid, carnitine C9:0, carnitine C7:0 and carnitine C9:0 isomer in plasma can be used as RFI biomarkers of male Tan sheep, and carnitine C18:2 can be used as RFI biomarker of female Tan sheep.

Cite this article

LU Tongtong , WANG Junkui , LUO Fang , HE Dongqian , CHEN Liyao , ZHANG Qian , WU Shaofei , GUO Yansheng , ZHANG Ruixue , TAO Jinzhong . Analysis of Plasma Metabolomics of Tan Sheep with Different Residual Feed Intake[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6930 -6940 . DOI: 10.3969/j.issn.1006-267x.2021.12.034

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