AQUATIC ANIMAL NUTRITION AND FEED

Effects of Dietary Black Soldier Fly Larvae Meal on Serum Metabolites, Antioxidant and Immune Indexes and Intestinal Histological Appearance of Micropterus salmoides

  • PENG Kai ,
  • XIAO Hongfa ,
  • MO Wenyan ,
  • WANG Guoxia ,
  • HUANG Yanhua
Expand
  • 1. Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture in Rural Affairs, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
    2. Guangzhou Fishtech Biotechnology Co., Ltd., Guangzhou 510640, China;
    3. Innovative Institute of Animal Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China

Received date: 2021-04-27

  Online published: 2021-12-16

Abstract

This experiment was aimed to study the effects of dietary black soldier fly larvae meal replacing fish meal on serum metabolites, antioxidant and immune indexes and intestinal histological appearance of Micropterus salmoides. A total of 525 juvenile Micropterus salmoides with an initial body weight of (4.9±0.1) g were divided into 5 groups with 3 replicates in each group and 35 fish in each replicate. Five isoproteic and isolipidic diets were formulated by adding black soldier fly larvae meal to replace 0 (G0), 7.5% (G7.5), 15.0% (G15.0), 22.5% (G22.5) and 30.0% (G30.0) of fish meal in diets. The feeding period was 62 days. Results showed as follows:compared with G0 group, serum albumin content in G15.0 to G30.0 groups was significantly increased (P<0.05), and serum globulin content in G22.5 and G30.0 groups was significantly increased (P<0.05), but cholesterol content and aspartate aminotransferase activity were significantly decreased (P<0.05); serum high density lipoprotein cholesterol content in G30.0 group were significantly increased (P<0.05);serum glutathion peroxidase activity and complement C3 content in G7.5 to G30.0 groups was significantly increased (P<0.05), and serum acid phosphatase activity in G15.0 to G30.0 groups was significantly increased (P<0.05). The intestine tissue in G7.5 to G30.0 groups had varying degrees of injury, and the higher replacement ratio of larvae meal to fish meal, the more serious damage of intestine tissue was. In conclusion, black soldier fly larvae meal replacing fish meal in diets significantly improves serum antioxidant and immunity, but damages liver function and intestinal histological appearance of Micropterus salmoides. Therefore, it is suggested that dietary supplementation of black soldier fly larvae meal should be cautious.

Cite this article

PENG Kai , XIAO Hongfa , MO Wenyan , WANG Guoxia , HUANG Yanhua . Effects of Dietary Black Soldier Fly Larvae Meal on Serum Metabolites, Antioxidant and Immune Indexes and Intestinal Histological Appearance of Micropterus salmoides[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 6964 -6972 . DOI: 10.3969/j.issn.1006-267x.2021.12.037

References

[1] HENRY M,GASCO L,PICCOLO G,et al.Review on the use of insects in the diet of farmed fish:past and future[J].Animal Feed Science and Technology,2015,203:1-22.
[2] SEALEY W M,GAYLORD T G,BARROWS F T,et al.Sensory analysis of rainbow trout,Oncorhynchus mykiss,fed enriched black soldier fly prepupae,Hermetia illucens[J].Journal of the World Aquaculture Society,2011,42(1):34-45.  
[3] LOCK E R,ARSIWALLA T,WAAGBØR.Insect larvae meal as an alternative source of nutrients in the diet of Atlantic salmon (Salmo salar) postsmolt[J].Aquaculture Nutrition,2016,22(6):1202-1213.  
[4] CUMMINS V C,RAWLES S D,THOMPSON K R,et al.Evaluation of black soldier fly (Hermetia illucens) larvae meal as partial or total replacement of marine fish meal in practical diets for Pacific white shrimp (Litopenaeus vannamei)[J]. Aquaculture,2017,473:337-344.
[5] HU J R,WANG G X,HUANG Y H,et al.Effects of substitution of fish meal with black soldier fly (Hermetia illucens) larvae meal,in yellow catfish (Pelteobagrus fulvidraco) diets[J].Israeli Journal of Aquaculture-Bamidgeh,2017,69:1-9.
[6] 胡俊茹,王国霞,莫文艳,等.黑水虻幼虫粉替代鱼粉对鲈鱼幼鱼生长性能、体组成、血浆生化指标和组织结构的影响[J].动物营养学报,2018,30(2):613-623. HU J R,WANG G X,MO W Y,et al.Effects of fish meal replacement by black soldier fly (Hermetia illucens l.) larvae meal on growth performance,body composition,plasma biochemical indexes and tissue structure of juvenile Lateolabrax japonicas[J].Chinese Journal of Animal Nutrition,2018,30(2):613-623.(in Chinese)
[7] ZHOU J S,LIU S S,JI H,et al.Effect of replacing dietary fish meal with black soldier fly larvae meal on growth and fatty acid composition of Jian carp (Cyprinus carpio var.Jian)[J].Aquaculture Nutrition,2018,24(1):424-433.  
[8] MAGALHAES R,SÁNCHEZ-LÓPEZBC A,LEAL R S,et al.Black soldier fly (Hermetia illucens) pre-pupae meal as a fish meal replacement in diets for European seabass (Dicentrarchus labrax)[J].Aquaculture,2017,476:79-85.
[9] RENNA M,SCHIAVONE A,GAI F,et al.Evaluation of the suitability of a partially defatted black soldier fly (Hermetia illucens L.) larvae meal as ingredient for rainbow trout (Oncorhynchus mykiss Walbaum) diets[J].Journal of Animal Science and Biotechnology,2017,8(4):57.
[10] XIAO X P,JIN P,ZHENG LY,et al.Effects of black soldier fly (Hermetia illucens) larvae meal protein as a fishmeal replacement on the growth and immune index of yellow catfish (Pelteobagrus fulvidraco)[J].Aquaculture Research,2018,49(4):1569-1577.  
[11] WANG G X,PENG K,HU J R,et al.Evaluation of defatted black soldier fly (Hermetia illucens L.) larvae meal as an alternative protein ingredient for juvenile Japanese seabass (Lateolabrax japonicus) diets[J].Aquaculture,2019,507:144-154.
[12] WANG G X,PENG K,HU JR,et al.Evaluation of defatted Hermetia illucens larvae meal for Litopenaeus vannamei:effects on growth performance,nutrition retention,antioxidant and immune response,digestive enzyme activity and hepatic morphology[J].Aquaculture Nutrition,2021,27(4):986-997.  
[13] LI S L,JI H,ZHANG B X,et al.Defatted black soldier fly (Hermetia illucens) larvae meal in diets for juvenile Jian carp (Cyprinus carpio var.Jian):growth performance,antioxidant enzyme activities,digestive enzyme activities,intestine and hepatopancreas histological structure[J].Aquaculture,2017,477:62-70.
[14] 胡俊茹,王国霞,黄文庆,等.黑水虻幼虫粉替代鱼粉对凡纳滨对虾幼虾生长性能、体组成、血清生化指标和抗氧化能力的影响[J].动物营养学报,2019,31(11):5293-5301. HU J R,WANG G X,HUANG W Q,et al.Effects of fish meal replacement by black soldier fly (Hermetia illucens) larvae meal on growth performance,body composition,serum biochemical indexes and antioxidant ability of juvenile Litopenaeus vannamei[J].Chinese Journal of Animal Nutrition,2019,31(11):5292-5300.(in Chinese)
[15] 彭凯,萧鸿发,莫文艳,等.黑水虻幼虫粉替代鱼粉对加州鲈生长性能、形体指标、体成分及营养物质沉积率的影响[J/OL].动物营养学报,(2021-06-23)[2021-08-15]:1-9. http://kns.cnki.net/kcms/detail/11.5461.S.20210623.0907.012.html. PENG K,XIAO H F,MO W Y,et al.Effects of replacing fish meal with black soldier fly larvae meal on growth performance,physical indexes,body composition and nutrient retention rates of Micropterus salmoides[J/OL].Chinese Journal of Animal Nutrition,(2021-06-23)[2021-08-15]:1-9. http://kns.cnki.net/kcms/detail/11.5461.S.20210623.0907.012.html.(in Chinese)
[16] 王爱民,韩光明,封功能,等.饲料脂肪水平对吉富罗非鱼生产性能、营养物质消化及血液生化指标的影响[J].水生生物学报,2011,35(1):80-87. WANG A M,HAN G M,FENG G N,et al.Effects of dietary lipid levels on growth performance,nutrient digestibility and blood biochemical indices of gift tilapia (Oreochromis niloticus)[J].Acta Hydrobiologica Sinica,2011,35(1):80-87.(in Chinese)
[17] JOHN P J.Alteration of certain blood parameters of freshwater teleost Mystus vittatus after chronic exposure to metasystox and Sevin[J].Fish Physiology and Biochemistry,2007,33(1):15-20.  
[18] 陈晓瑛,胡俊茹,王国霞,等.黑水虻幼虫粉替代鱼粉对黄颡鱼幼鱼生长性能、肌肉品质及血清生化指标的影响[J].动物营养学报,2019,31(6):2788-2799. CHEN X Y,HU J R,WANG G X,et al.Effects of fish meal replacement by black soldier fly (Hermetia illucens) larvae meal on growth performance,serum biochemical indices and meat quality of juvenile yellow catfish (Pelteobagrus fulvidraco)[J].Chinese Journal of Animal Nutrition,2019,31(6):2788-2799.(in Chinese)
[19] 张放,杨伟丽,杨树义,等.黑水虻虫粉对生长猪生长性能和血清生化指标的影响[J].猪业观察,2018(3):34-38. ZHANG F,YANG W L,YANG S Y,et al.Effects of black soldier fly meal on growth performance and serum biochemical parameters of growing pigs[J].Swine Industry Outlook,2018(3):34-38.(in Chinese)
[20] 王国霞,陈冰,孙育平,等.脱脂亮斑扁角水虻幼虫粉替代鱼粉对黄颡鱼幼鱼生长性能、营养素沉积率、血清生化指标和消化酶活性的影响[J].水产学报,2020,44(6):987-998. WANG G X,CHEN B,SUN Y P,et al.Effects of replacing fish meal with defatted black soldier fly (Hermetia illucens) larvae meal on growth performance,nutrient retention,serum biochemical parameters and digestive enzymes activity of juvenile Pelteobagrus fulvidraco[J].Journal of Fisheries of China,2020,44(6):987-998.(in Chinese)
[21] JIN M,LU Y,YUAN Y,et al.Regulation of growth,antioxidant capacity,fatty acid profiles,hematological characteristics and expression of lipid relatedgenes by different dietary n-3 highly unsaturated fatty acidsin juvenile black seabream (Acanthopagrus schlegelii)[J].Aquaculture,2017,471:55-65.
[22] CHEN Y,ZHU X,YANG Y,et al.Effect of dietary chitosan on growth performance,haematology,immune response,intestine morphology,intestine microbiota and disease resistancein gibel carp (Carassius auratus gibelio)[J].Aquaculture Nutrition,2014,20(5):532-546.  
[23] 赵聘,赵云焕.复合抗热应激添加剂对蛋鸡血液生化指标的影响[J].河南农业科学,2005,2(2):70-73. ZHAO P,ZHAO Y H.Effect of complicated anti-heat stress additives on blood biochemical indexes of layers[J].Journal of Henan Agricultural Sciences,2005(2):70-73.(in Chinese)
[24] XU X X,JI H,YU H B,et al.Influence of dietary black soldier fly (Hermetia illucens Linnaeus) pulp on growth performance,antioxidant capacity and intestinal health of juvenile mirror carp(Cyprinus carpio var.specularis)[J].Aquaculture Nutrition,2020,26(2):432-443.  
[25] URAL M Ş,YONAR M E,MIŞE Y S.Protective effect of ellagic acid on oxidative stress and antioxidant status in Cyprinus carpio during malathion exposure[J].Cellular and Molecular Biology(Noisy-le-Grand,France),2015,61(5):58-63.
[26] KROECKEL S,HARJES A G E,ROTH I,et al.When a turbot catches a fly:evaluation of a pre-pupae meal of the black soldier fly (Hermetia illucens) as fish meal substitute-growth performance and chitin degradation in juvenile turbot (Psetta maxima)[J].Aquaculture,2012,364/365:345-352.
[27] 胡俊茹,何飞,莫文艳.采食不同有机废弃物黑水虻幼虫饲料价值分析[J].中国饲料,2017(15):24-27. HU J R,HE F,MO W Y.The feeding value of black soldier fly (Hermetia illucens) larvae for feeding different organic wastes[J].China Feed,2017(15):24-27.(in Chinese)
[28] NGO D N,LEE S H,KIM M M,et al.Production of chitin oligosaccharides with different molecular weights and their antioxidant effect in RAW 264.7 cells[J].Journal of Functional Foods,2009,1(2):188-198.  
[29] NGO D H,KIM S K.Antioxidant effects of chitin,chitosan,and their derivatives[J].Advances in Food and Nutrition Research,2014,73:15-31.
[30] PENG K,CHEN X Y,WEI D,et al.Inclusion of Chlorella water extract in Oreochromis niloticus fingerling diets:effects on growth performance,body composition,digestive enzyme activity,antioxidant and immune capacity,intestine and hepatic histomorphology and sodium nitrite stress resistance[J].Aquaculture Reports,2020,18:100547.
[31] WEI J T,WANG S X,LIU G,et al.Polysaccharides from Enteromorpha prolifera enhance the immunity of normal mice[J].International Journal of Biological Macromolecules,2014,64:1-5.
[32] SANCHEZ-MUROS M J,BARROSO F G,MANZANO-AGUGLIARO F.Insect meal as renewable source of food for animal feeding:a review[J].Journal of Cleaner Production,2014,65:16-27.
[33] DONG X Q,ZHANG D M,CHEN Y K,et al.Effects of antimicrobial peptides (AMPs) on blood biochemical parameters,antioxidase activity,and immune function in the common carp (Cyprinus carpio)[J].Fish & Shellfish Immunology,2015,47(1):429-434.  
[34] ZARANTONIELLO M,BRUNI L,RANDAZZO B,et al.Partial dietary inclusion of Hermetia illucens (black soldier fly) full-fat prepupae in zebrafish feed:biometric, histological,biochemical,and molecular implications[J].Zebrafish,2018,15(5):519-532.  
[35] DUMAS A,RAGGI T,BARKHOUSE J,et al.The oil fraction and partially defatted meal of black soldier fly larvae (Hermetia illucens) affect differently growth performance,feed efficiency,nutrient deposition,blood glucose and lipid digestibility of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2018,492:24-34.
[36] POMA G,CUYKX M,AMATO E,et al.Evaluation of hazardous chemicals in edible insects and insect-based food intended for humanconsumption[J].Food and Chemical Toxicology,2017,100:70-79.
Outlines

/