SPECIAL ECONOMIC ANIMAL NUTRITION AND FEED

Effects of Bacillus subtilis on Intestinal Structure, Cecal Volatile Fatty Acid Contents and Microbial Diversity of Meat Rabbits

  • WEI Yuchao ,
  • WANG Fengxia ,
  • ZHANG Can ,
  • SUN Dongyue ,
  • ZHAO Yuping ,
  • LI Ning ,
  • YAN Juntong ,
  • CHEN Baojiang
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  • 1. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
    2. Cattle and Sheep Embryo Technology Innovation Center of Hebei Province, Baoding 071000, China

Received date: 2021-05-11

  Online published: 2021-12-16

Abstract

This experiment was conducted to investigate the effects of Bacillus subtilis on intestinal structure, cecal volatile fatty acid contents and microbial diversity of meat rabbits. Eighty 30-day-old healthy meat rabbits with similar body weight[(1.15±0.05) kg] were randomly divided into 4 groups with 20 replicates per group and 1 rabbit per replicate. Rabbits in the control group were fed a basal diet, and the others in test groups were fed the basal diets supplemented with 100, 200 and 300 g/t Bacillus subtilis, respectively. The pre-test period lasted for 7 days, and the formal test period lasted for 30 days. The results showed as follows:1) compared with the control group, the average daily gain and average daily feed intake of test group Ⅰ were significantly increased (P<0.05). 2) The villus height in jejunum of test group Ⅰ was significantly higher than that of the control group (P<0.01), the villus height/crypt depth in jejunum of test group Ⅲ was significantly lower than that of test group Ⅰ (P<0.01), and the villus height in ileum of test group Ⅲ was significantly lower than that of control group and test group Ⅰ (P<0.05). 3) Compared with the control group, the pH in cecum of test group Ⅲ was significantly increased (P<0.05). 4) Compared with the control group, the operational taxonomic unit (OUT) number and Chao1 index of cecal microorganism of test group Ⅰ were significantly increased (P<0.01), while the OTU number and Chao1 index of test group Ⅲ were significantly decreased (P<0.01). 5) At the phylun level, compared with the control group, the Firmicutes relative abundance of test group Ⅲ was significantly decreased (P<0.01), the Bacteroidetes relative abundance of test group Ⅲ was significantly increased (P<0.01), and the Proteobacteria relative abundance of test groups Ⅰ and Ⅱ was significantly decreased (P<0.01). At the genus level, compared with the control group, the Ruminococcaceae NK4A214 group relative abundance of test groups was significantly decreased (P<0.05), the Christensenellaceae R-7 group relative abundance of test groups was significantly decreased (P<0.01), the Ruminococcaceae UCG-014 relative abundance of test group Ⅰ was significantly increased (P<0.01), the Akkermansia relative abundance of test group Ⅰ was significantly decreased (P<0.01), and the Subdoligranulum relative abundance of test group Ⅰ was significantly decreased (P<0.01). In conclusion, the dietary appropriate dose of Bacillus subtilis (100 g/d) is beneficial to increase the diversity of cecal microorganisms and improve intestinal health and growth performance of meat rabbits, excessive addition of Bacillus subtilis will have negative effects on intestinal health of meat rabbits.

Cite this article

WEI Yuchao , WANG Fengxia , ZHANG Can , SUN Dongyue , ZHAO Yuping , LI Ning , YAN Juntong , CHEN Baojiang . Effects of Bacillus subtilis on Intestinal Structure, Cecal Volatile Fatty Acid Contents and Microbial Diversity of Meat Rabbits[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 7021 -7032 . DOI: 10.3969/j.issn.1006-267x.2021.12.042

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