EXPERIMENTAL METHOD AND ANIMAL

Effects of Astragalin on Expression of miR-155 in Liver Tissue of Mice after Acute Exhaustive Exercise

  • ZHU Kaiye ,
  • WU Qiuxue ,
  • LUO Haijing ,
  • ZHANG Weiwei ,
  • SHAO Shuli
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  • 1. School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China;
    2. Key Laboratory of Resistant Genetic Engineering and Cold Biodiversity Conservation of Heilongjiang Province, Qiqihar 161006, China

Received date: 2021-05-06

  Online published: 2021-12-16

Abstract

In order to study the effects of astragalin on the expression of miR-155 in liver tissue of mice after acute exhaustive exercise. Forty specific pathogen free (SPF) 8-week-old c57BL/6J male mice were randomly divided into 4 groups:DMSO group, exhaustion exercise group, 50 mg/kg astragalin group and 100 mg/kg astragalin group. In addition to the DMSO group, the mice of other three groups received exhaustive exercise for 4 h. The mice of the astragalin group were given 50 and 100 mg/kg astragalin after exhaustive exercise, respectively, those in the exhaustion exercise group were given the same amount of normal saline after exhaustive exercise, and those in the DMSO group were given the same amount of 0.1% DMSO which prepared with normal saline, and the animals were sacrificed for 1 hour. The serum and liver tissue were collected, the activity of alanine aminotransferase (ALT) in serum and the activity of anti-superoxide anion free radical (ASAFR) in liver tissue were determined. The expression of oxidation-related miRNAs in liver tissue were detected by real-time quantitative fluorescence PCR (qRT-PCR). The results showed that, compared with the DMSO group, the activity of ALT in serum of mice after acute exhaustive exercise was increased by16.03 times (P<0.05), the activity of ASAFR in liver tissue of mice was decreased by 43% (P<0.05), which indicated that acute exhaustive exercise caused oxidative damage to the liver of mice. Compared with the DMSO group, the relative expression level of miR-155 was increased by 6.29 times in liver tissue of mice after acute exhaustive exercise (P<0.01). After gavage 50 mg/kg of astragalin, compared with the exhaustive exercise group, the activity of ALT in serum was decreased by 77% (P<0.05), the activity of ASAFR in liver tissue was increased by 1.77 times (P<0.05), and the relative expression level of miR-155 was decreased by 66% (P<0.05). After gavage 100 mg/kg astragalin, compared with the exhaustive exercise group, the activity of ALT in serum was decreased by 91% (P<0.05), the activity of ASAFR in liver tissue was increased by 1.95 times (P<0.05), and the relative expression level of miR-155 was decreased by 60% (P<0.05). These results indicate that the hepatic oxidative damage induced by acute exhaustive exercise in mice can be partially repaired by astragalin.

Cite this article

ZHU Kaiye , WU Qiuxue , LUO Haijing , ZHANG Weiwei , SHAO Shuli . Effects of Astragalin on Expression of miR-155 in Liver Tissue of Mice after Acute Exhaustive Exercise[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 7062 -7069 . DOI: 10.3969/j.issn.1006-267x.2021.12.046

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