Analysis Potential Roles of miRNA in Regulation of Milk Protein Synthesis in Mammary Tissue of Heat Stressed Dairy Cows Based on RNA Sequencing

GAO Shengtao; MA Lu; BU Dengpan

doi:10.3969/j.issn.1006-267x.2021.12.048

2021 , Vol. 33 >Issue 12: 7078 - 7091

DOI: https://doi.org/10.3969/j.issn.1006-267x.2021.12.048

MOLECULAR AND CELLULAR NUTRITION

Analysis Potential Roles of miRNA in Regulation of Milk Protein Synthesis in Mammary Tissue of Heat Stressed Dairy Cows Based on RNA Sequencing

GAO Shengtao ,
MA Lu ,
BU Dengpan

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State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2021-04-09

Online published: 2021-12-16

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Abstract

The objective of the present study was to evaluate the differently expressed miRNA in dairy cows' mammary tissue and its potential roles in regulation of milk protein synthesis. Four healthy Holstein cows with similar lactation age, body weight and milk yield were selected as experimental animals. A 2×2 crossover design was used, the experiment included two periods, each period included pre-test period and test period for 9 days, respectively, and the interval between two periods was 30 days. The experiment was carried out in the artificial environment control cabin. The pre-test period was a hot neutral environment and free feeding. Cows in test period were randomly divided into two groups:heat stress group (HS group, n=4) and pair-feeding restriction group (PFTN group, n=4). The results showed as follows:1) sixteen differently expressed miRNA were identified in dairy cows' mammary tissue between HS group and PFTN group (Padj<0.05). 2) In differently expressed miRNA, bta-miR-451 might involve in the regulation of energy metabolism, bta-miR-184, bta-miR-218 and bta-miR-493 might involve in the regulation of cell proliferation, bta-miR-132 might involve in the regulation of inflammation response, while bta-miR-382 might have synergistic effect with the regulation in mRNA expression in mammary tissue. In conclusion, miRNA in mammary tissue may involve in the regulation of energy metabolism, cell proliferation and inflammatory response, this study lays a theoretical and scientific foundation for further studies to confirm the regulatory effect of miRNA on milk protein synthesis under heat stress.

Key words： heat stress; milk protein; mammary tissue; miRNA

Cite this article

GAO Shengtao , MA Lu , BU Dengpan . Analysis Potential Roles of miRNA in Regulation of Milk Protein Synthesis in Mammary Tissue of Heat Stressed Dairy Cows Based on RNA Sequencing[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 7078 -7091 . DOI: 10.3969/j.issn.1006-267x.2021.12.048

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