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Effects of Bacillus subtilis on Growth Performance, Serum Biochemical Parameters and Organ Development of Weaned Bamboo Rats

  • BAN Bo ,
  • DAI Wei ,
  • ZHANG Haitao ,
  • TANG Hailin ,
  • ZHANG Jinyu ,
  • LENG Dianbo ,
  • SUN Jielong ,
  • TIAN Kexiong
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  • 1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Agricultural Science Research Institute of Yiyang, Yiyang 413046, China;
    3. Xiangxi Jinlifeng Agricultural Development Company, Huayuan 416400, China

Received date: 2021-04-27

  Online published: 2021-12-16

Abstract

The purpose of this experiment was to study the effects of Bacillus subtilis on growth performance, serum biochemical parameters and organ development of weaned bamboo rats. A single factor experiment design was adopted, ninety-six 40-day-old weaned hoary bamboo rats with the same body weight and good health condition were selected and divided into 4 groups with 6 replicates per group and 4 rats per replicate (half male and half female). Rats in the control group were fed a basic diet, and the others in test groups Ⅰ, Ⅱ and Ⅲ were fed basic diets supplemented with 2.22×1012, 4.44×1012 and 6.66×1012 CFU/kg Bacillus subtilis, respectively. The test period was 60 days, and the first 30 days was the early period. The results showed as follows:1) in the early stage of the test, compared with the control group, the average daily feed intake (concentrates) of test group Ⅰ, Ⅱ and Ⅲ showed an increase trend (0.05<P<0.10). In the whole period of the test, compared with the control group, the average daily feed intake (concentrates) of test groups Ⅰ, Ⅱ and Ⅲ was significantly increased (P<0.01); the final body weight and average daily gain of test group Ⅲ were significantly increased (P<0.05), and the feed to gain ratio (roughages) and feed to gain ratio (total) were significantly decreased (P<0.05). The survival rate of test groups Ⅰ, Ⅱ and Ⅲ was increased by 15.79% higher compared with the control group (P>0.05). 2) Compared with the control group, the contents of creatinine, low-density lipoprotein and total cholesterol in serum of test group Ⅲ showed a decrease trend (0.05<P<0.10), and the activity of aspartate aminotransferase and contents of uric acid, triglycerides and calcium in serum were significantly decreased (P<0.05). 3) Compared with the control group, the liver index, spleen index, kidney index and thymus index of test group Ⅲ were significantly increased (P<0.05). In conclusion, adding Bacillus subtilis to the diet can effectively improve the survival rate and growth performance of weaned bamboo rats, and promote the organ development. The addition of 6.66×1012 CFU/kg Bacillus subtilis to the diet has the best growth performance of weaned bamboo rats.

Cite this article

BAN Bo , DAI Wei , ZHANG Haitao , TANG Hailin , ZHANG Jinyu , LENG Dianbo , SUN Jielong , TIAN Kexiong . Effects of Bacillus subtilis on Growth Performance, Serum Biochemical Parameters and Organ Development of Weaned Bamboo Rats[J]. Chinese Journal of Animal Nutrition, 2021 , 33(12) : 7192 -7200 . DOI: 10.3969/j.issn.1006-267x.2021.12.058

References

[1] 班博,戴维,王敏,等.竹鼠的生物学特征与饲养管理[J].经济动物学报,2020,24(1):52-56,62. BAN B,DAI W,WANG M,et al.Biological characteristics and feeding management of bamboo rats[J].Journal of Economic Animal,2020,24(1):52-56,62.(in Chinese)
[2] 班博,戴维,田科雄.枯草芽孢杆菌的生理功能及其在动物生产中的应用[J].湖南饲料,2020(2):42-45. BAN B,DAI W,TIAN K X.Physiological function of Bacillus subtilis and its application in animal production[J].Hunan Feed,2020(2):42-45.(in Chinese)
[3] 王晓丹,孔祥峰,赵越,等.枯草芽孢杆菌对断奶仔猪生长性能和血浆生化参数的影响[J].动物营养学报,2019,31(2):605-611. WANG X D,KONG X F,ZHAO Y,et al.Effects of Bacillus subtilis on growth performance and plasma biochemical parameters of weaned piglets[J].Chinese Journal of Animal Nutrition,2019,31(2):605-611.(in Chinese)
[4] 张婷,冯培祥,王卓,等.枯草芽孢杆菌对断奶驴驹生长性能、营养物质表观消化率及肠道菌群的影响[J].动物营养学报,2020,32(10):4956-4962. ZHANG T,FENG P X,WANG Z,et al.Effects of Bacillus subtilis on growth performance, nutrient apparent digestibility and intestinal flora of weaned donkey foals[J].Chinese Journal of Animal Nutrition,2020,32(10):4956-4962.(in Chinese)
[5] MA Y B,WANG W W,ZHANG H J,et al.Supplemental Bacillus subtilis DSM 32315 manipulates intestinal structure and microbial composition in broiler chickens[J].Scientific Reports,2018,8(1):15358.
[6] 黄玉岚,霍小东,姚宏明,等.枯草芽孢杆菌WEI-62体外益生评价及其对仔猪生长性能,肠道形态和肠道菌群的影响[J].中国畜牧杂志,2020,56(11):140-145. HUANG Y L,HUO X D,YAO H M,et al.In vitro probiotic evaluation of Bacillus subtilis WEI-62 and its effect on growth performance,intestinal morphology and intestinal flora of piglets[J].Chinese Journal of Animal Science,2020,56(11):140-145.(in Chinese)
[7] NGUYEN A T,NGUYEN D V,TRAN M T,et al.Isolation and characterization of Bacillus subtilis CH16 strain from chicken gastrointestinal tracts for use as a feed supplement to promote weight gain in broilers[J].Letters in Applied Microbiology,2015,60(6):580-588.  
[8] SOBCZAK A,KOZLOWSKI K.The effect of a probiotic preparation containing Bacillus subtilis ATCC PTA-6737 on egg production and physiological parameters of laying hens[J].Annals of Animal Science,2015,15(3):711-723.  
[9] GUO J R,DONG X F,LIU S,et al.Effects of long-term Bacillus subtilis CGMCC 1.921 supplementation on performance,egg quality, and fecal and cecal microbiota of laying hens[J].Poultry Science,2017,96(5):1280-1289.  
[10] 张娟利,莫放,姚刚,等.日粮添加枯草芽孢杆菌对13月龄新疆褐牛育肥增重性能和消化率的影响[J].中国畜牧杂志,2020,56(12):153-157. ZHANG J L,MO F,YAO G,et al.Effect of dietary Bacillus subtilis supplementation on feedlotting performance and nutrient digestibility of Xinjiang brown beef cattle at initial age of thirteen month[J].Chinese Journal of Animal Science,2020,56(12):153-157.(in Chinese)
[11] SIU-RODAS Y,CALIXTO-ROMO M L A,GUILLÉN-NAVARRO K,et al.Bacillus subtilis with endocellulase and exocellulase activities isolated in the thermophilic phase from composting with coffee residues[J].Revista Argentina de Microbiologia,2018,50(3):234-243.  
[12] HU Y,YU D,WANG Z,et al.Purification and characterization of a novel,highly potent fibrinolytic enzyme from Bacillus subtilis DC27 screened from Douchi,a traditional Chinese fermented soybean food[J].Scientific Reports,2019,9(1):9235.
[13] GAO Z H,WU H H,SHI L,et al.Study of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d broiler chickens[J].Animal Nutrition,2017,3(2):109-113.  
[14] LIU Y L,YAN T,LI X Y,et al.Effects of Bacillus subtilis and antibiotic growth promoters on the growth performance,intestinal function and gut microbiota of pullets from 0 to 6 weeks[J].Animal,2020,2(28):1-10.
[15] HU L,PENG X,CHEN H,et al.Effects of intrauterine growth retardation and Bacillus subtilis PB6 supplementation on growth performance, intestinal development and immune function of piglets during the suckling period[J].European Journal of Nutrition,2017,56(4):1753-1765.  
[16] FORTE C,MOSCATI L,ACUTI G,et al.Effects of dietary Lactobacillus acidophilus and Bacillus subtilis on laying performance, egg quality, blood biochemistry and immune response of organic laying hens[J].Journal of Animal Physiology and Animal Nutrition,2016,100(5):977-987.  
[17] WANG B G,XU H B,WEI H,et al.Oral administration of Bifidobacterim bifidum for modulating microflora, acid and bile resistance, and physiological indices in mice[J].Canadian Journal of Microbiology,2014,61(2):155-163.
[18] HATAB M H,ELSAYED M A,IBRAHIM N S.Effect of some biological supplementation on productive performance physiological and immunological response of layer chicks[J].Journal of Radiation Research and Applied Sciences,2016,9(2):185-192.  
[19] 刘俊阳,高爱武,成立新,等.复合微生态制剂对犊牛生长性能、粪样微生物和血液指标的影响[J].畜牧与饲料科学,2019,40(4):21-25,64. LIU J Y,GAO A W,CHENG L X,et al.Effects of dietary supplementation of a compound micro-ecological preparation on growth performance,number of fecal microbes and serum indexes of calves[J].Animal Husbandry and Feed Science,2019,40(4):21-25,64.(in Chinese)
[20] 李小芬,杨晓志,陶勇,等.微生态制剂对樱桃谷肉鸭生长性能及血液生化指标的影响[J]. 农业科学与技术,2015,16(8):1732-1734,1740. LI X F,YANG X Z,TAO Y,et al.Effects of probiotics on growth performance and blood biochemical indexes of cherry valley ducks[J].Agricultural Science & Technology,2015,16(8):1732-1734,1740.(in Chinese)
[21] 邢月腾,李丽立,王升平,等.枯草芽孢杆菌PL83对育雏期临武鸭生长性能和血清生化指标的影响[J].湖南农业大学学报(自然科学版),2014,40(3):294-298,320. XING Y T,LI L L,WANG S P,et al.Effects of Bacillus subtilis PL83 on growth performance and serum biochemical parameters of Linwu ducks[J].Journal of Hunan Agricultural University (Natural Sciences Edition),2014,40(3):294-298,320.(in Chinese)
[22] FUKUSHIMA M,NAKANO M.The effect of a probiotic on faecal and liver lipid classes in rats[J].British Journal of Nutrition,1995,73(5):701-710.  
[23] 陈彩文,张晓慧,曹赞,等.枯草芽孢杆菌对1-21日龄AA鸡生长性能、血液生化指标及肠道组织结构的影响[J].饲料工业,2015,36(19):10-14. CHEN C W,ZHANG X H,CAO Z,et al.Effects of Bacillus subtilis on growth performance, blood biochemical parameters and small intestine histological morphology in 1 to 21 d AA broilers[J].Feed Industry,2015,36(19):10-14.(in Chinese)
[24] MAROUFYAN E,KASIM A,HASHEMI S R,et al.Change in growth performance and liver function enzymes of broiler chickens challenged with infectious bursal disease virus to dietary supplementation of methionine and threonine[J].American Journal of Animal and Veterinary Sciences,2010,5(1):20-26.  
[25] PETRIE H T.Role of thymic organ structure and stromal composition in steady-state postnatal T-cell production[J].Immunological Reviews,2002,189:8-19.
[26] 吕远蓉,王怀禹,李彩虹,等.复合微生态制剂对竹鼠生产性能的影响[J].黑龙江畜牧兽医,2019(12):141-143. LV Y R,WANG H Y,LI C H,et al.The effect of compound probiotics on the production performance of bamboo rats[J].Heilongjiang Animal Science and Veterinary Medicine,2019(12):141-143.(in Chinese)
[27] 郭梦娇.枯草芽孢杆菌的筛选及其诱导兔抗大肠杆菌感染先天性免疫应答机制研究[D].博士学位论文.泰安:山东农业大学,2018. GUO M J.Screening of Bacillus subtilis and its mechanism of inducing the innate immune response to anti-infection Escherichia coli in rabbit[D]Ph.D.Thesis.Tai'an:Shandong Agricultural University,2018.(in Chinese)
[28] 郭瑞萍,肖发沂,都振玉,等.枯草芽孢杆菌对肉鸡生产性能、屠宰性能、免疫性能和肌肉风味的影响[J].中国饲料,2019(17):79-83. GUO R P,XIAO F Y,DU Z Y,et al.Effects of Bacillus subtilis on the production performance,slaughter performance,immune performance and muscle flavor of broiler chickens[J].China Feed,2019(17):79-83.(in Chinese)
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