Effects of Different Phosphorus Sources on Growth Performance, Serum Indices, Bone Calcium and Phosphorus Metabolism and Intestinal Microbes of Finishing Pigs

LIU Jing; CHEN Bingdian; HE Shuying; QIU Hualing; MIAO Furong; DONG Zhiyan; LI Zhongrong; FANG Guiyou; YE Dingcheng; CHEN Zhuo; CHEN Xinzhu

doi:10.3969/j.issn.1006-267x.2022.01.011

2022 , Vol. 34 >Issue 1: 104 - 114

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.011

RESEARCH PAPER

Effects of Different Phosphorus Sources on Growth Performance, Serum Indices, Bone Calcium and Phosphorus Metabolism and Intestinal Microbes of Finishing Pigs

LIU Jing ,
CHEN Bingdian ,
HE Shuying ,
QIU Hualing ,
MIAO Furong ,
DONG Zhiyan ,
LI Zhongrong ,
FANG Guiyou ,
YE Dingcheng ,
CHEN Zhuo ,
CHEN Xinzhu

Expand

1. Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China;
2. Sinochem Yunlong Co., Ltd., Chengdu 655204, China;
3. School of Business Administration, Fujian College of Commerce, Fuzhou 350016, China;
4. Postdoctoral Workstation of Fujian Xinminke Biology Science and Technology Development Co., Ltd., Fuzhou 350008, China

Received date: 2021-06-04

Online published: 2022-01-18

Fold

Abstract

This experiment was conducted to study the effects of different phosphorus sources on growth performance, serum indices, bone calcium and phosphorus metabolism and intestinal microbes of finishing pigs. A total of 640 healthy "Duroc×Landrace×Yorkshire" crossbred pigs with the same genetic background and initial body weight of (63.10±2.32) kg were randomly divided into 5 groups with 8 replicates per group and 16 pigs (half male and half female) per replicate. The control group was fed a basal diet, and the phosphorus source was calcium hydrogen phosphate (DCP); the phosphorus source in experimental group 1 was mono-dicalcium phosphate (MDCP), and the level of phosphorus provided by MDCP was 80% of that provided by DCP in the control group; the phosphorus source in experimental group 2 was MDCP, and the level of phosphorus provided by MDCP was 60% of that provided by DCP in the control group; the phosphorus source in experimental group 3 was monocalcium phosphate (MCP), and the level of phosphorus provided by MCP was 80% of that provided by DCP in the control group; the phosphorus source in experimental group 4 was MCP, and the level of phosphorus provided by MCP was 60% of that provided by DCP in the control group. The experiment lasted for 90 days, including a pre-experiment period of 10 days and a formal experiment period of 80 days. The results showed as follows:1) compared with the control group, the final body weight of finishing pigs in experimental groups 1 and 4 was significantly increased (P<0.05), the average daily gain in experimental group 1 and the apparent phosphorus digestibility in experimental group 2 were significantly increased (P<0.05). 2) Compared with the control group, the insulin-like growth factor-Ⅰ (IGF-Ⅰ) content in muscle, liver and serum of finishing pigs in experimental groups was significantly increased (P<0.05), and the growth hormone (GH) content in muscle and serum was also significantly increased (P<0.05). And the contents of IGF-Ⅰ and GH in muscle in experimental groups 3 and 4 were significantly higher than those in experimental groups 1 and 2 (P<0.05). The IGF-Ⅰ relative expression level in muscle in experimental group 2 was significantly increased (P<0.05), the IGF-Ⅰ relative expression level in liver in experimental group 3 was significantly decreased (P<0.05), and the GH relative expression level in muscle in experimental group 4 was significantly higher than that in experimental group 3 (P<0.05). 3) Different phosphorus sources had certain effects on the species and quantity of microorganisms in each section of intestinal tract of finishing pigs. The microbial diversity in jejunum and ileum was increased, especially the jejunum had more newly added bacteria, while the cecum had more stable microorganisms. In conclusion, MDCP or MCP supplemented with 80% or 60% replacement ratio can improve the growth performance and intestinal microbial diversity of finishing pigs to a certain extent, and is a good phosphorus source to replace DCP.

Key words： monocalcium phosphate; mono-dicalcium phosphate; finishing pigs; growth performance; serum indices; calcium and phosphorus metabolism; intestinal microbes

Cite this article

LIU Jing , CHEN Bingdian , HE Shuying , QIU Hualing , MIAO Furong , DONG Zhiyan , LI Zhongrong , FANG Guiyou , YE Dingcheng , CHEN Zhuo , CHEN Xinzhu . Effects of Different Phosphorus Sources on Growth Performance, Serum Indices, Bone Calcium and Phosphorus Metabolism and Intestinal Microbes of Finishing Pigs[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 104 -114 . DOI: 10.3969/j.issn.1006-267x.2022.01.011

References

[1] 计成.动物营养学[M].北京:高等教育出版社,2011:119-126. JI C.Animal nutrition[M].Beijing:Higher Education Press,2011:119-126.(in Chinese)
[2] 刘景,陈炳钿,李忠荣,等.不同添加水平的磷酸一二钙对肉鸡生产性能、钙磷代谢及盲肠微生物的影响[J].畜牧兽医学报,2020,51(6):1306-1319. LIU J,CHEN B D,LI Z R,et al.Effects of different dietary mono-dicalcium phosphate supplementation on production performance,blood parameters,bone calcium,phosphorus metabolism and cecal microecology in broilers[J].Acta Veterinaria et Zootechnica Sinica,2020,51(6):1306-1319.(in Chinese)
[3] 李文静.磷源与磷水平对肉鸭磷相对生物学利用率的研究[D].硕士学位论文.邯郸:河北工程大学,2018. LI W J.Research on relative biological utilization rate of phosphorus sources and levels in the ducks[D].Master's Thesis.Handan:Hebei University of Engineering,2018.(in Chinese)
[4] 敖翔,何健.磷在肉仔鸡上的营养作用及其生物学利用率的研究进展[J].饲料与畜牧,2018(5):59-64. AO X,HE J.Research progress of phosphorus's nutritional effect on broilers and its biological utilization[J].Feed and Husbandry,2018(5):59-64.(in Chinese)
[5] 王秀静.不同无机磷源对断奶仔猪生长性能、肠道形态结构及肠道稳态的影响[D].硕士学位论文.南昌:江西农业大学,2015. WANG X J.Regulation of different inorganic phosphorus source on growth performance,intestinal morphology structure and intestinal steady state of weaned piglets[D].Master's Thesis.Nanchang:Jiangxi Agricultural University,2015.(in Chinese)
[6] 张永成,李德发,邢建军.新型饲料在断奶仔猪中的应用[J].中国动物保健,1999(4):30,32. ZHANG Y C,LI D F,XING J J.Application of new feed in weaning piglets[J].China Animal Health,1999(4):30,32.(in Chinese)
[7] STEIN H H,KADZERE C T,KIM S W,et al.日粮磷水平对生长育肥猪日粮中磷酸二氢钙磷消化率的影响[J].饲料广角,2014(12):12-15. STEIN H H,KADZERE C T,KIM S W,et al.Influence of dietary phosphorus concentration on the digestibility of phosphorus in monocalcium phosphate by growing pigs[J].Feed China,2014(12):12-15.(in Chinese)
[8] 张忠远,徐宝成.饲料中营养物质表观消化率的计算公式[J].饲料博览,2004(4):48. ZHANG Z Y,XU B C.The formula for calculating the apparent digestibility of nutrients in feed[J].Feed Review,2004(4):48.(in Chinese)
[9] LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2^-ΔΔCt method[J].Methods,2001,25(4):402-408.

[10] 夏良宙,李霞,万荣.不同种类和来源磷酸钙盐对肉鸡生产性能和养分代谢的影响[J].饲料工业,2014(S1):48-52. XIA L Z,LI X,WAN R.Influence of phosphorus source on growth performance and nutrient utilization of broilers[J].Feed Industry,2014(S1):48-52.(in Chinese)
[11] 屠焰,范先国,霍启光.影响磷酸盐中磷生物学利用率的因素[J].中国饲料,1998(9):6-8. TU Y,FAN X G,HUO Q G.The factors of affecting biological utilization of phosphorus in phosphate[J].China Feed,1998(9):6-8.(in Chinese)
[12] 曹慧.猪对饲料级磷酸盐磷生物学利用率的研究[D].硕士学位论文.雅安:四川农业大学,2003. CAO H.The research of relative bioavailability of phosphorus in feed phosphates[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2003.(in Chinese)
[13] BERTELSON A.Altemate phosphours source valuable for growing swine[J].Feedsutffs,1982,53(40):12-13.
[14] 黄阿彬.不同磷源及水平对育肥猪生长性能和骨骼质量的影响[D].硕士学位论文.雅安:四川农业大学,2013. HUANG A B.Effects of dietary phosphorus level and phosphorus sources on growth performance and tibia quality of finishing pigs[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2013.(in Chinese)
[15] 王晓鹃,林海.我国蛋鸡营养与饲料研究进展[J].动物营养学报,2020,32(10):4602-4610. WANG X J,LIN H.Recent progress in nutrition and feed of laying hens in China[J].Chinese Journal of Animal Nutrition,2020,32(10):4602-4610.(in Chinese)
[16] 胡龙昌,项智锋,方热军.磷在动物小肠中的吸收转运及其调控机制的研究进展[J].饲料与畜牧,2012(10):34-36. HU L C,XIANG Z F,FANG R J. Research progress on absorption and transport of phosphorus in small intestine and its regulatory mechanism[J].Feed and Husbandry,2012(10):34-36.(in Chinese)
[17] DILGER R N,ADEOLA O.Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing pigs fed conventional and low-phytate soybean meals[J].Journal of Animal Science,2006,84(3):627-634.

[18] YÁÑEZ J L,BELTRANENA E,CERVANTES M,et al.Effect of phytase and xylanase supplementation or particle size on nutrient digestibility of diets containing distillers dried grains with solubles cofermented from wheat and corn in ileal-cannulated grower pigs[J].Journal of Animal Science,2011,89(1):113-123.

[19] BOHLKE R A,THALER R C,STEIN H H.Calcium,phosphorus,and amino acid digestibility in low-phytate corn,normal corn,and soybean meal by growing pigs[J].Journal of Animal Science,2005,83(10):2396-2403.

[20] 代述均.饲粮非植酸磷水平对肉鸭生产性能、盲肠微生物及胫骨质量的影响[D].硕士学位论文.雅安:四川农业大学,2017. DAI S J.Effect of dietary non-phytate phosphorus levels on performance,caecal microflora and tibia characteristics in meat ducks[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2017.(in Chinese)
[21] 孙杰.断奶仔猪颗粒料加工工艺比较研究[D].硕士学位论文.北京:中国农业科学院,2014. SUN J.Comparative studies of the different processing technologies on the weaned piglet's feed pellet[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2014.(in Chinese)
[22] 刘静.饲粮磷来源和水平以及生长阶段对猪后肠磷消化的影响[D].硕士学位论文.绵阳:西南科技大学,2020. LIU J. Effects of dietary phosphorus sources,concentration and growth stage on postileal phosphorus digestion in pigs[D].Master's Thesis.Mianyang:Southwest University of Science and Technology,2020.(in Chinese)

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References