Study on Fungi Structure in Ileum and Colon of Jinhua Pigs and Its Correlation with Body Fat Deposition

LI Jingshang; ZHANG Xiaojun; CHEN Shengchang; XIANG Yun; XU E; LOU Fangfang; YANG Hua; XIAO Yingping

doi:10.3969/j.issn.1006-267x.2022.01.014

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 1: 131 - 140

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.014

RESEARCH PAPER

Study on Fungi Structure in Ileum and Colon of Jinhua Pigs and Its Correlation with Body Fat Deposition

LI Jingshang ,
ZHANG Xiaojun ,
CHEN Shengchang ,
XIANG Yun ,
XU E ,
LOU Fangfang ,
YANG Hua ,
XIAO Yingping

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1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-Product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;
3. Institute of Animal Husbandry and Veterinary Medicine, Jinhua Academy of Agricultural Sciences, Jinhua 321017, China

Received date: 2021-06-27

Online published: 2022-01-18

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Abstract

The aim of this study was to explore the differences of the fungi flora structure in the ileum and colon of Jinhua pigs with different fatness rates and to determine its correlation with body fat deposition. A total of 35 Jinhua pigs of the same batch at the age of 270 days were selected for rearing, the backfat thickness, ribeye area and lean meat rate were measured. According to the lean meat rate, five Jinhua pigs with the lowest (high fat group, H group) and highest (low fat group, L group) lean meat rate were selected for slaughter. The colon and ileum contents were collected to further analyze the fungi sequencing analysis. The results showed as follows:1) Jinhua pigs with different fatness rates displayed significant difference in lean meat rate and backfat thickness (P<0.05), and there was no significant difference in body weight and ribeye area. 2) The richness and diversity of fungi flora of Jinhua pigs in L group was higher than those in H group. In addition, the principal coordinate analysis also found that the two groups of fungi flora exhibited different clusters, indicating differences in fungi flora structure. At the phylum level, Ascomycota was the dominant fungi in the ileum and colon of Jinhua pigs in H group, and Basidiomycota was the dominant fungi in the ileum and colon of Jinhua pigs in L group. At the genus level, the dominant fungi in the ileum and colon of Jinhua pigs in H group were Kazachstania and Clavaria, respectively, and their relative abundances were 85.74% and 32.87% respectively; the dominant fungi in the ileum and colon of Jinhua pigs in L group were Naganishia and Xerochrysium, respectively, and their relative abundances were 75.62% and 68.53%, respectively. Heat map analysis showed that the two intestinal segments had two different fungi in common, one was Kazachstania, which presented in the intestinal tract of Jinhua pigs in H group, the other was Naganishia, which existed in the intestinal tract of Jinhua pigs in L group. 3) Spearman correlation analysis found that Kazachstania, Saccharomyces, Aspergillus were all highly positively correlated with backfat thickness (r=0.59 to 0.92), Naganishia, Clavaria and Rhizopus were all negatively correlated with backfat thickness (r=-0.88 to -0.78). It can be seen that the intestinal fungi structure of Jinhua pigs with different fatness rates is different, which also shows a certain correlation with backfat thickness, indicating the association between intestinal fungi changes and host fat deposition.

Key words： Jinhua pigs; lean meat rate; backfat thickness; fungi structure; fat deposition

Cite this article

LI Jingshang , ZHANG Xiaojun , CHEN Shengchang , XIANG Yun , XU E , LOU Fangfang , YANG Hua , XIAO Yingping . Study on Fungi Structure in Ileum and Colon of Jinhua Pigs and Its Correlation with Body Fat Deposition[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 131 -140 . DOI: 10.3969/j.issn.1006-267x.2022.01.014

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