Effects of Galacto-Oligosaccharides on Cecal Microflora, Intestinal Inflammation and Barrier Function of Suckling Piglets Stimulated by Lipopolysaccharides

GAO Ren; TIAN Shiyi; WANG Jing; ZHU Weiyun

doi:10.3969/j.issn.1006-267x.2022.01.018

2022 , Vol. 34 >Issue 1: 177 - 189

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.018

RESEARCH PAPER

Effects of Galacto-Oligosaccharides on Cecal Microflora, Intestinal Inflammation and Barrier Function of Suckling Piglets Stimulated by Lipopolysaccharides

GAO Ren ,
TIAN Shiyi ,
WANG Jing ,
ZHU Weiyun

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National Center for International Research on Animal Gut Nutrition, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received date: 2021-05-27

Online published: 2022-01-18

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Abstract

The purpose of this study was to investigate the effects of galacto-oligosaccharides (GOS) on cecal microbiota, intestinal inflammation and barrier function of suckling piglets stimulated by lipopolysaccharides (LPS). Eighteen newborn suckling piglets with similar initial body weight of (1.57±0.05) kg were selected and randomly divided into control group, LPS stimulated group and GOS supplemental group, and with 6 piglets in each group. Piglets in the GOS supplemental group were fed with 1 g/kg BW GOS solution every day, and piglets in the control group and LPS stimulated group were received an equivalent volume of sterile saline. On the 14th day, piglets in the LPS stimulated group and GOS supplemental group were intraperitoneally injected with LPS solution of 80 μg/kg BW for challenge, and piglets in the control group were intraperitoneally injected with sterile saline of the same amount. All piglets were sampled at slaughter 2 h after intraperitoneally injected. The results showed as follows:1) compared with LPS stimulated group, the Shannon index of GOS supplemental group was tended to increase (P=0.051); at the phylum level, the cecal digesta Firmicutes relative abundance of GOS supplemental group was significantly increased (P<0.05), and at the genus level, the relative abundances of[Eubacterium]_coprostanoligenes_group, Phascolarctobacterium, Prevotella_2, Lachnospiraceae_NK4A136_group and Faecalibacterium in cecal digesta of GOS supplemental group were significantly increased (P<0.05 or P<0.01). 2) Compared with LPS stimulated group, the contents of acetate, propionate, butyrate and total short chain fatty acids in cecal digesta of GOS supplemental group were significantly increased (P<0.05 or P<0.01), and the cecal digesta pH was significantly decreased (P<0.05). 3) Compared with LPS stimulated group, the contents of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in cecal mucosa of GOS supplemental group were significantly decreased (P<0.05). 4) Compared with LPS stimulated group, the protein expression level of claudin-1 in cecal mucosa of GOS supplemental group was significantly decreased (P<0.05), and the zonula occludens-1 (ZO-1) protein expression level was tend to increased (P=0.075). In conclusion, the early GOS intervention can modulate the cecal microbiota structure of suckling piglets stimulated by LPS, increase the cecal digesta short chain fatty acid contents, and relieve intestinal inflammation and barrier function caused by LPS.

Key words： galacto-oligosaccharides; lipopolysaccharides; suckling piglets; cecal microbiota; intestinal inflammation; barrier function

Cite this article

GAO Ren , TIAN Shiyi , WANG Jing , ZHU Weiyun . Effects of Galacto-Oligosaccharides on Cecal Microflora, Intestinal Inflammation and Barrier Function of Suckling Piglets Stimulated by Lipopolysaccharides[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 177 -189 . DOI: 10.3969/j.issn.1006-267x.2022.01.018

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