Effects of Dietary Supplementation with Different Levels of Vitamin A on Immune Function and Metabolism of Nitric Oxide in Liver and Duodenum of Broilers

LI Yinhao; ZHANG Qingyue; GUO Xiaoyu; YAN Sumei; SHI Binlin; ZHAO Yanli

doi:10.3969/j.issn.1006-267x.2022.01.022

2022 , Vol. 34 >Issue 1: 228 - 236

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.022

RESEARCH PAPER

Effects of Dietary Supplementation with Different Levels of Vitamin A on Immune Function and Metabolism of Nitric Oxide in Liver and Duodenum of Broilers

LI Yinhao ,
ZHANG Qingyue ,
GUO Xiaoyu ,
YAN Sumei ,
SHI Binlin ,
ZHAO Yanli

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Inner Mongolia Autonomous Region Key Laboratory of Animal Nutrition and Feed Science, Collage of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received date: 2021-05-25

Online published: 2022-01-18

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Abstract

In this study, the effects of dietary vitamin A supplementation on immune function of broilers were studied, and the content of nitric oxide (NO) in the liver and duodenum and the relative expression level of inducible nitric oxide synthase (iNOS) mRNA were also tested. A total of 240 one-day-old Arbor Acres (AA) broiler chickens (half male and half female) were randomly allotted into five groups with six replicates of eight broilers in each replicate. The trial lasted for 42 days. Five different levels of vitamin A were added to basic diets in each group, with levels of 3 000, 6 000, 15 000, 30 000 and 45 000 IU/kg vitamin A, respectively. The results showed as follows:1) the dietary vitamin A had a significant effect on the bursa of Fabricius index of broilers at 21 and 42 days of age (P=0.078,P=0.060). The bursa of Fabricius index of the 6 000 and 15 000 IU/kg groups was the highest, and the 45 000 IU/kg group was the lowest. 2) Vitamin A levels had a significant effect on the serum contents of interleukin-1 (IL-1) of broiler at 21 and 42 days of age and interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) of broiler at 42 days of age (P<0.05), and with the increase of vitamin A level, there was a significant quadratic curve decreasing dose-dependent relationship (P<0.05), and the content was higher in the 15 000 IU/kg group and lower in the 45 000 IU/kg group; At 42 days of age, the level of dietary vitamin A tended to have a significant effect on the serum interleukin-2 content of broilers (P=0.075), and there was a significant linear increase with the increase of dietary vitamin A level (P<0.05). 3) With the increase of dietary vitamin A level, the liver nitric oxide content showed a significant quadratic curve decrease (P<0.05), and 6 000 IU/kg group was the highest; iNOS, total nitric oxide synthase (tNOS) activity and iNOS mRNA relative expression level showed a significant linear decrease (P<0.05). The enzyme activity and gene expression of the 30 000 and 45 000 IU/kg groups were significantly lower than those of the other groups (P<0.05).The changes of NO content, iNOS, tNOS activities and iNOS mRNA relative expression level in the duodenum were similar to those in the liver. In summary, the dietary supplementation with vitamin A of 6 000 to 15 000 IU/kg promotes the development of the immune function of the broilers. The NO content, iNOS, tNOS activities and iNOS mRNA relative expression level in the liver and duodenum of broilers have a dose effect with vitamin A, which is low level increases NO content and up-regulates the iNOS mRNA relative expression level, while high level shows inhibitory effect.

Key words： vitamin A; broiler; immune function; NO; iNOS gene

Cite this article

LI Yinhao , ZHANG Qingyue , GUO Xiaoyu , YAN Sumei , SHI Binlin , ZHAO Yanli . Effects of Dietary Supplementation with Different Levels of Vitamin A on Immune Function and Metabolism of Nitric Oxide in Liver and Duodenum of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 228 -236 . DOI: 10.3969/j.issn.1006-267x.2022.01.022

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