Effects of Days of Age and Dietary Barley Worm Meal on Intestinal Structure, Tight Junction Protein and Defense Peptide mRNA Expression of Yellow-Feathered Broilers

YU Qinping; ZHANG Cuiping; HUANG Jingyu; LIU Jing; HUANG Yuanbin; YU Ting; ZHANG Xiaoai; WANG Lei; CHEN Jinsheng; CHEN Wenqing; WANG Zhilin; CHEN Zhuang; RONG Ting

doi:10.3969/j.issn.1006-267x.2022.01.023

2022 , Vol. 34 >Issue 1: 237 - 253

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.023

RESEARCH PAPER

Effects of Days of Age and Dietary Barley Worm Meal on Intestinal Structure, Tight Junction Protein and Defense Peptide mRNA Expression of Yellow-Feathered Broilers

YU Qinping ,
ZHANG Cuiping ,
HUANG Jingyu ,
LIU Jing ,
HUANG Yuanbin ,
YU Ting ,
ZHANG Xiaoai ,
WANG Lei ,
CHEN Jinsheng ,
CHEN Wenqing ,
WANG Zhilin ,
CHEN Zhuang ,
RONG Ting

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1. Key Laboratory of Conservation and Utilization of Crop Germplasm Resources in Guangdong Province, Agricultural Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
2. Guangzhou Zhiteqi Biotechnology Co., Ltd., Guangzhou 510700, China;
3. Guangdong Haida Group, Guangzhou 511400, China;
4. Guangdong Chongchong Biotechnology Co., Ltd., Guangzhou 511462, China;
5. Raoping Animal Health Supervision Institute, Chaozhou 515700, China;
6. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangdong Public Laboratory of Animal Breeding and Nutrition, Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, State Key Laboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Received date: 2021-06-22

Online published: 2022-01-18

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Abstract

This experiment was conducted to investigate the changes of intestinal structure and functional development during early development of yellow-feathered broilers and the effects of dietary barley worm meal on their intestinal structure and functional development. Ninety healthy yellow-feathered broilers of one-day-old were randomly divided into 3 groups with 6 replicates per group and 5 broilers per replicate. Broilers in the control group were fed a basal diet, those in the antibiotic group were fed the basal diet supplemented with 300 g/t 15% aureomycin and 40 g/t 50% virginiamycin, and those in the barley worm meal group were fed the basal diet supplemented with 3% barley worm meal. The nutrient levels of diets in the three groups were the same, and the experiment lasted for 7 days. The results showed as follows:1) from 1 to 7 days of age, the total length of small intestine and the relative length of ileum of yellow-feathered broilers were significantly increased with the increase of days of age (P<0.05); the villus height, crypt depth and the ratio of villus height to crypt depth (V/C) of duodenum were significantly increased (P<0.05); the villus height, crypt depth and goblet cell number of jejunum were significantly increased (P<0.05), and the V/C was significantly increased first (P<0.05) and then decreased; the villus height and goblet cell number of ileum were significantly increased (P<0.05). 2) From 1 to 7 days of age, the mRNA relative expression levels of claudin-1 and β-defense peptide 8 (AvBD8) in duodenum and AvBD8 in ileum of yellow-feathered broilers were significantly decreased with the increase of age (P<0.05). The mRNA relative expression levels of polyimmunoglobulin receptor (pIgR) in duodenum and mucin 2 (MUC2), pIgR and NK-lysin in ileum were significantly increased (P<0.05). 3) Compared with the control group, dietary 3% barley worm meal tended to increase the V/C of duodenum and zonula occluden-1 (ZO-1) mRNA relative expression level in ileum of yellow-feathered broilers (P>0.05). In conclusion, from 1 to 7 days of age, the total length of small intestine, villus height and goblet cell number of yellow-feathered broilers increase rapidly, the mRNA relative expression levels of claudin-1 and AvBD8 in duodenum and AvBD8 in ileum decrease gradually, and the mRNA relative expression levels of pIgR in duodenum and MUC2, pIgR and NK-lysin in ileum increase gradually. Dietary 3% barley worm meal can increase the V/C of duodenum and promote the ZO-1 mRNA expression in ileum of yellow-feathered broilers, suggesting that barley worm meal can improve the nutrition and congenital intestinal development of yellow-feathered broilers.

Key words： barley worm meal; chicks; intestinal structure; small intestine development; tight junction protein; defense peptide

Cite this article

YU Qinping , ZHANG Cuiping , HUANG Jingyu , LIU Jing , HUANG Yuanbin , YU Ting , ZHANG Xiaoai , WANG Lei , CHEN Jinsheng , CHEN Wenqing , WANG Zhilin , CHEN Zhuang , RONG Ting . Effects of Days of Age and Dietary Barley Worm Meal on Intestinal Structure, Tight Junction Protein and Defense Peptide mRNA Expression of Yellow-Feathered Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 237 -253 . DOI: 10.3969/j.issn.1006-267x.2022.01.023

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