RESEARCH PAPER

Effects of Combinatorial Protease on Feed Intake Preference, Serum Biochemical Indices and Intestinal Tissue Morphology of Broilers

  • WANG Xinxin ,
  • LI Xixi ,
  • LYU Yi ,
  • MA Wenfeng ,
  • WU Xiaohong ,
  • ZHAO Furong
Expand
  • College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471023, China

Received date: 2021-06-18

  Online published: 2022-01-18

Abstract

This experiment was conducted to investigate the effects of combinatorial protease on feed intake preference, serum biochemical indices and intestinal tissue morphology of broilers. In experiment 1, a total of 40 healthy Arbor Acres (AA) broilers at 1 day of age with similar body condition were randomly divided into 4 treatments with 10 broilers in each treatment. Broilers in four treatments were fed a basal diet (diet Ⅰ) and the basal diet supplemented with 4 (diet Ⅱ), 8 (diet Ⅲ) and 12 U/g (diet Ⅳ) combinatorial protease at the same time. And the feed intake preference of broilers to different diets was measured. In experiment 2, a total of 240 healthy AA broilers at 1 day of age with similar body condition were randomly divided into 4 groups with 6 replicates per group and 10 broilers per replicate. Broilers in the control group (group Ⅰ) were fed a basal diet, and those in the experimental groups (groups Ⅱ to Ⅳ) were fed the basal diet supplemented with 4, 8 and 12 U/g combinatorial protease, respectively. And the serum biochemical indices and intestinal tissue morphology of broilers were measured. The chickens were free to eat and drink, and both experiments lasted for 42 days. The results showed as follows:1) there were no significant differences in feed consumption, feed intake times, total feed intake time and feed intake time per meal of 4 kinds of diets among broilers in all treatments (P>0.05). From 22 to 42 days of age, the feed intake percentage of diet Ⅱ for broilers was significantly higher than that of the other three diets (P<0.05). 2) From 1 to 21 days of age, the serum contents of total protein (TP), globulin (GLB), total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol of broilers in group Ⅳ were significantly higher than those in group Ⅰ (P<0.05). From 22 to 42 days of age, the serum contents of TP and GLB of broilers in group Ⅲ were significantly higher than those in group Ⅰ (P<0.05), and the serum thyroxine content in group Ⅲ was significantly lower than that in group Ⅰ (P<0.05). 3) The crypt depth in jejunum and duodenum of broilers in group Ⅱ was significantly lower than that in group Ⅲ (P<0.05), the villus height to crypt depth ratio (V/C) in jejunum was significantly higher than that in groups Ⅲ and Ⅳ (P<0.05), and V/C in duodenum was significantly higher than that in groups Ⅰ and Ⅲ (P<0.05). But compared with group Ⅰ, the villus height in ileum in group Ⅱ was significantly decreased (P<0.05). In conclusion, broilers only showed a feed intake preference to the diet supplemented with 4 U/g combinatorial protease at the later growth period (22 to 42 days of age); dietary 12 U/g combinatorial protease can improve lipid synthesis and enhance immunity of broilers at 21 days of age; dietary 8 U/g combinatorial protease can significantly increase the contents of TP and GLB in serum of broilers at 42 days of age; and dietary 4 U/g combinatorial protease can improve or maintain the intestinal tissue morphology of broilers. The optimum supplemental level of combinatorial protease is 8 U/g in this experiment after comprehensive consideration.

Cite this article

WANG Xinxin , LI Xixi , LYU Yi , MA Wenfeng , WU Xiaohong , ZHAO Furong . Effects of Combinatorial Protease on Feed Intake Preference, Serum Biochemical Indices and Intestinal Tissue Morphology of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 264 -273 . DOI: 10.3969/j.issn.1006-267x.2022.01.025

References

[1] 姜润深.我国肉鸡福利养殖的关注点和发展方向[J].中国禽业导刊,2019,36(22):16-17. JIANG R S.The focus and development direction of broiler welfare breeding in China[J].Guide to Chinese Poultry,2019,36(22):16-17.(in Chinese)
[2] 唐彩琰,王晶晶,罗静如,等.蛋白酶在动物饲料中的应用[J].国外畜牧学(猪与禽),2020,40(9):70-71. TANG C Y,WANG J J,LUO J R,et al.Proteases-animal feed[J].Animal Science Abroad (Pigs and Poultry),2020,40(9):70-71.(in Chinese)
[3] MOHAMMADIGHEISAR M,KIM I H.Addition of a protease to low crude protein density diets of broiler chickens[J].Journal of Applied Animal Research,2018,46(1):1377-1381.  
[4] 谭权,孙得发.外源蛋白酶对蛋鸡生产性能及经济效益的影响[J].中国畜牧杂志,2018,54(3):83-86. TAN Q,SUN D F.Effect of CIBENZA DP100 on performance and benefits of layers[J].Chinese Journal of Animal Science,2018,54(3):83-86.(in Chinese)
[5] 王长平,缪天琳,刘德江,等.蛋白质水平对育成期蛋鸡啄羽和其它行为的影响[J].粮食与饲料工业,2017(6):44-45,56. WANG C P,MIAO T L,LIU D J,et al.Effects of protein level on feather pecking and other behaviors of laying hens during incubation period[J].Cereal & Feed Industry,2017(6):44-45,56.(in Chinese)
[6] 刘胜利,周闯,刘文龙,等.外源蛋白酶对肉鸡生长、消化和血液生化指标的影响[J].饲料研究,2019,42(1):44-48. LIU S L,ZHOU C,LIU W L,et al.Effects of exogenous protease on growth,digestion and blood biochemical indexes of broilers[J].Feed Research,2019,42(1):44-48.(in Chinese)
[7] 李东东.不同蛋白质水平饲粮添加蛋白酶对肉鸡生产性能和消化生理的影响[D].硕士学位论文.雅安:四川农业大学,2015. LI D D.Effect of adding protease in different protein levels diets on performance and digestive physiology of broilers[D].Master's Thesis.Yaan:Sichuan Agricultural University,2015.(in Chinese)
[8] 侯振平,吴端钦,蒋桂韬,等.不同蛋白酶对肉鸡屠宰性能、器官指数、肠道形态结构及养分消化率的影响[J].家畜生态学报,2018,39(1):25-30. HOU Z P,WU D Q,JIANG G T,et al.Effects of different protease supplementation on slaughter performance,organ index,intestinal villi structure and nutrient metabolizability of broilers[J].Acta Ecologae Animalis Domastici,2018,39(1):25-30.(in Chinese)
[9] 张东龙,赵芙蓉,武晓红,等.不同光周期对肉仔鸡采食行为、生长性能及血液指标的影响[J].动物营养学报,2020,32(5):2176-2184. ZHANG D L,ZHAO F R,WU X H,et al.Effects of different photoperiods on feeding behavior,growth performance and blood parameters of broilers[J].Chinese Journal of Animal Nutrition,2020,32(5):2176-2184.(in Chinese)
[10] 韩淑云.日粮中添加可溶性纤维对蛋鸡采食行为、产蛋性能和蛋品质的影响[J].中国饲料,2020(10):53-55. HAN S Y.The effect of dietary soluble fiber on the feeding behavior,laying performance and egg quality of laying hens[J].China Feed,2020(10):53-55.(in Chinese)
[11] AMER S A,NASER M A F,ABDEL-WARETH A A A,et al.Effect of dietary supplementation of alpha-galactosidase on the growth performance,ileal digestibility,intestinal morphology,and biochemical parameters in broiler chickens[J].BMC Veterinary Research,2020,16(1):144.
[12] MOLOKANOVA O V,SHATSKIKH E V.Biochemical composition of broiler chickens blood when including the proteolytic enzyme Sibenza DP 100 in the diet[J].Perm Agrarian Journal (Permskij Agrarnyj Vestnik),2019,3(27):108-116.
[13] 吴琼,宁浩然,宋超,等.我国不同家养雉鸡血液生化指标测定与比较分析[J].畜牧与饲料科学,2018,39(11):24-26,30. WU Q,NING H R,SONG C,et al.Determination and comparative analysis of blood biochemical indexes of different domestic pheasant populations in China[J].Animal Husbandry and Feed Science,2018,39(11):24-26,30.(in Chinese)
[14] 魏莲清,牛俊丽,赵官正,等.添加不同水平的发酵棉粕对科宝肉鸡生长性能、屠宰性能和血清生化指标的影响[J].中国畜牧兽医,2019,46(7):1953-1961. WEI L Q,NIU J L,ZHAO G Z,et al.Effects of different levels of fermented cottonseed meal on growth performance,slaughter performance and serum biochemical indexes of Cobb broilers[J].China Animal Husbandry & Veterinary Medicine,2019,46(7):1953-1961.(in Chinese)
[15] 张金金,王瑞华,张邦,等.黑水虻幼虫粉对蛋鸡产蛋后期生产性能、蛋品质及血液生理生化指标的影响[J].动物营养学报,2020,32(4):1658-1665. ZHANG J J,WANG R H,ZHANG B,et al.Effects of black soldier fly larvae meal on performance,egg quality and blood physiological and biochemical parameters of hens during late laying period[J].Chinese Journal of Animal Nutrition,2020,32(4):1658-1665.(in Chinese)
[16] ZHANG Q Y,ZHANG S,CONG G L,et al.Effects of soy protein concentrate in starter phase diet on growth performance,blood biochemical indices, carcass traits,immune organ indices and meat quality of broilers[J].Animals,2021,11(2):281.
[17] 侯振平,蒋桂韬,吴端钦,等.蛋白酶对1-21日龄肉仔鸡生长性能、血清生化指标及抗氧化功能的影响[J].饲料工业,2017,38(14):17-20. HOU Z P,JIANG G T,WU D Q,et al.Effects of protease on growth performance,serum biochemical index and antioxidant function of 1 to 21 d broilers[J].Feed Industry,2017,38(14):17-20.(in Chinese)
[18] 殷璐瑶.饲养密度对仔鹅生长、肉用性能及福利状况的影响[D].硕士学位论文.扬州:扬州大学,2018. YIN L Y.Effects of stocking density on growth,meat performance,and welfare of geese[D].Master's Thesis.Yangzhou:Yangzhou University,2018.(in Chinese)
[19] 徐静,张子儒,王德贺,等.饮水中添加大蒜精油对蛋鸡生长性能、肠道组织形态及盲肠菌群的影响[J].动物营养学报,2021,33(1):308-316. XU J,ZHANG Z R,WANG D H,et al.Effects of adding garlic essential oil into drinking water on growth performance,intestinal tissue morphology and cecum microbial flora of layer hens[J].Chinese Journal of Animal Nutrition,2021,33(1):308-316.(in Chinese)
[20] OMAR A E,AL-KHALAIFAH H S,MOHAMED W A M,et al.Effects of phenolic-rich onion (Allium cepa L.) extract on the growth performance,behavior,intestinal histology,amino acid digestibility,antioxidant activity,and the immune status of broiler chickens[J].Frontiers in Veterinary Science,2020,7:582612.
[21] GRACIA M I,LÁZARO R,LATORRE M A,et al.Influence of enzyme supplementation of diets and cooking-flaking of maize on digestive traits and growth performance of broilers from 1 to 21 days of age[J].Animal Feed Science and Technology,2009,150(3/4):303-315.
[22] 周梁.外源蛋白酶(ProAct)对肉鸡生产性能及氨基酸消化率影响的研究[D].硕士学位论文.北京:中国农业科学院,2014. ZHOU L.The Study of a exogenous protease (ProAct) on performance and amino acids digestibility in broiler chicken[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2014.(in Chinese)
[23] 侯振平,蒋桂韬,王满生,等.蛋白酶对22-42日龄肉鸡屠宰性能、肠道形态结构和养分消化率的影响[J].畜牧与兽医,2018,50(1):47-53. HOU Z P,JIANG G T,WANG M S,et al.Effects of protease on slaughter performance,intestinal villi structure and nutrient digestibility of 22-to 42-day-old broilers[J].Animal Husbandry & Veterinary Medicine,2018,50(1):47-53.(in Chinese)
[24] 龙俊海,陈灵.蛋白酶与益生菌混合发酵豆粕对肉鸡生长性能及免疫功能的影[J].中国饲料,2020(19):118-121. LONG J H,CHEN L.Effect of mixed fermentation of protease and probiotics on growth performance and immune function of broiler[J].China Feed,2020(19):118-121.(in Chinese)
Outlines

/