Effects of Feeding 25-Hydroxyvitamin D3 and Vitamin D3 on Frozen Semen Quality and Nutrient Apparent Digestibility of Holstein Breeding Bulls at Different Stages

FENG Guanzhi; XU Hongjian; WANG Lihua; ZHANG Chengrui; ZU Zhifu; ZHANG Yonggen

doi:10.3969/j.issn.1006-267x.2022.01.035

2022 , Vol. 34 >Issue 1: 372 - 380

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.035

RESEARCH PAPER

Effects of Feeding 25-Hydroxyvitamin D₃ and Vitamin D₃ on Frozen Semen Quality and Nutrient Apparent Digestibility of Holstein Breeding Bulls at Different Stages

FENG Guanzhi ,
XU Hongjian ,
WANG Lihua ,
ZHANG Chengrui ,
ZU Zhifu ,
ZHANG Yonggen

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1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
2. Shanghai Dairy Cattle Breeding Center Co., Ltd., Shanghai 200436, China

Received date: 2021-06-20

Online published: 2022-01-18

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Abstract

This experiment was conducted to compare the effects of 25-hydroxyvitamin D₃ (25VD₃) and vitamin D₃ (VD₃) on frozen semen quality, antioxidant capacity, calcium, phosphorus and magnesium metabolism and nutrient apparent digestibility of young and adult Holstein bulls. Using a 2×2 two-factor experiment design (different forms of VD₃ and different stages of bulls), the different forms of VD₃ were 25VD₃ and VD₃. There were 16 adult Holstein bulls at 30 months of age and 16 young Holstein bulls at 12 months of age. Four groups were set up:adult bulls VD₃ group (300 IU/kg), adult bulls 25VD₃ group (300 IU/kg), young bulls VD₃ group (300 IU/kg), young bulls 25VD₃ group (300 IU/kg). The pre-feeding period was 14 days, and the trial period was 60 days. Semen, urine and feces were collected on days 1, 30 and 60 of the trial period. The results showed as follows:1) 25VD₃ significantly improved sperm motility and membrane integrity of thawed semen (P<0.05), there was a tendency to increase sperm survival rate (P=0.08). Sperm motility and survival rate of thawed semen of adult bulls were significantly higher than those of young bulls (P<0.05). 2) 25VD₃ tended to decrease the content of malondialdehyde in thawed semen of breeding bulls (P=0.07). The activity of catalase of thawed semen of adult bulls was significantly higher than that in young bulls (P<0.05). 3) 25VD₃ had a tendency to increase the apparent digestibility of crude protein (P=0.09) and significantly increased the apparent digestibility of calcium (P<0.05), and significantly reduced the discharge of urinary phosphorus and magnesium (P<0.05). The intake, deposition and fecal discharge of calcium, phosphorus and magnesium and urinary phosphorus in adult bulls were significantly higher than those in young bulls (P<0.01). In summary, dietary supplementation of 25VD₃ can increase the frozen semen sperm motility, membrane integrity and apparent digestibility of calcium and reduce the urine phosphorus and magnesium emissions of breeding bulls.

Key words： vitamin D₃; 25-dihydroxyvitamin D₃; Holstein breeding bulls; frozen semen quality; antioxidant capacity; minerals

Cite this article

FENG Guanzhi , XU Hongjian , WANG Lihua , ZHANG Chengrui , ZU Zhifu , ZHANG Yonggen . Effects of Feeding 25-Hydroxyvitamin D₃ and Vitamin D₃ on Frozen Semen Quality and Nutrient Apparent Digestibility of Holstein Breeding Bulls at Different Stages[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 372 -380 . DOI: 10.3969/j.issn.1006-267x.2022.01.035

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