Effects of Carvacrol and Thymol on Nutrient Apparent Digestibility, Rumen Fermentation Characteristics and Cellulose- Decomposed Bacteria Counts of Sheep

LIU Lishan; ZHOU Rui; WU Jianping; LANG Xia; WANG Cailian

doi:10.3969/j.issn.1006-267x.2022.01.045

2022 , Vol. 34 >Issue 1: 478 - 487

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.045

RESEARCH PAPER

Effects of Carvacrol and Thymol on Nutrient Apparent Digestibility, Rumen Fermentation Characteristics and Cellulose- Decomposed Bacteria Counts of Sheep

LIU Lishan ,
ZHOU Rui ,
WU Jianping ,
LANG Xia ,
WANG Cailian

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1. Key Laboratory for Sheep, Goat, and Cattle Germplasm and Straw Feed in Gansu Province, Animal Husbandry, Pasture and Green Agriculture Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China;
2. Life Science and Engineering College of Northwest University for Nationalities, Lanzhou 730070, China

Received date: 2021-06-25

Online published: 2022-01-18

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Abstract

This study was carried out to study the effects of carvacrol and thymol on nutrient apparent digestibility, rumen fermentation characteristics and cellulose-decomposed bacteria counts of sheep. Nine small tail Han sheep rams with permanent ruminal fistulas were randomly assigned to a 3×3 Latin square design with 20-day period. The pre-experimental period lasted for 5 days, and the experimental period lasted for 15 days. The treatments were as follows:sheep in the control group (CON group) were fed a basal diet, those in the test 1 group (T₁ group) were fed the basal diet supplemented with carvacrol at 2 g/d in the concentrate, and those in the test 2 group (T₂ group) were fed the basal diet supplemented with thymol at 2 g/d in the concentrate. Digestion and metabolism trial was carried using total feces collection method on the 10th day of the experimental period, and nutrient apparent digestibility was measured. Last day of the experimental, rumen fluid was collected before feeding (0 h) and 1, 3, 5 and 7 h after feeding to measure fermentation parameters and cellulose-decomposed bacteria counts. The results showed as follows:1) the concentration of ammoniacal nitrogen (NH₃-N) in all groups was at the highest level 1 h after feeding, rumen fluid pH was at the lowest level 3 h after feeding, while the total volatile fatty acids (TVFA) concentration in rumen fluid was the highest, the rumen fluid microprotein (MCP)concentration was at a higher level 3 and 5 h after feeding, the counts of Ruminococcus flavefaciens, Fibrobacter succinogenes in rumen fluid were the highest 3 h after feeding, and the count of Ruminococcus ablus was the highest 1 h after feeding. 2) On average value, T₁ group compared with the CON group, the concentrations of rumen fluid MCP and TVFA were significantly increased (P<0.05), rumen fluid pH was significantly decreased (P<0.05), the apparent digestibility of dry matter and crude protein were significantly increased (P<0.05), the counts of rumen fluid Ruminococcus flavefaciens, Fibrobacter succinogenes were significantly increased (P<0.05), and the count of Ruminococcus ablus was significantly decreased (P<0.05). T₂ group compared with the CON group, the concentrations of MCP and TVFA were significantly increased (P<0.05), but all nutrient apparent digestibility was not significantly changed (P>0.05), and the counts of rumen fluid Ruminococcus ablus was significantly decreased (P<0.05). In summary, carvacrol and thymol both can affect the rumen fermentation characteristics and cellulose-decomposed bacteria counts of sheep, but carvacrol also can significantly improve the apparent digestibility of dry matter and crude protein.

Key words： carvacrol; thymol; nutrient apparent digestibility; rumen fermentation characteristics; cellulose-decomposed bacteria

Cite this article

LIU Lishan , ZHOU Rui , WU Jianping , LANG Xia , WANG Cailian . Effects of Carvacrol and Thymol on Nutrient Apparent Digestibility, Rumen Fermentation Characteristics and Cellulose- Decomposed Bacteria Counts of Sheep[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 478 -487 . DOI: 10.3969/j.issn.1006-267x.2022.01.045

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