RESEARCH PAPER

Effects of Different Cereal Starches on Cecal Microflora Diversity of Weaned Foals

  • LI Qian ,
  • HUANG Xinxin ,
  • ZHANG Wenjie ,
  • TANGNAER· ,
  • Tuoliubieke ,
  • MA Chen ,
  • ZANG Changjia ,
  • CHEN Kaixu ,
  • LI Xiaobin
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  • Xinjiang Herbivore Nutrition Laboratory for Meat and Milk, Xinjiang Agricultural University, Urumqi 830052, China

Received date: 2021-05-25

  Online published: 2022-01-18

Abstract

The purpose of this experiment was to study the effects of different cereal starches on cecal microflora diversity of weaned foals, and to provide a theoretical basis for studying the biological effects of different cereal starches in regulating the intestinal microflora. Eighteen 5-month-old weaned Kazakh foals were randomly divided into 3 groups according to the principle of similar average body weight[(112.36±7.50) kg], each group had 6 horses. Under the same roughage conditions, with the same grain starch intake as the principle, each foal supplemented with 2 g (dry matter basis) cereal starch per kilogram of body weight, trial group Ⅰ was supplemented with the concentrate supplement which contained the steam pressed corn, trial group Ⅱ was supplemented with the concentrate supplement which contained the steam pressed oats, and trial group Ⅲ was supplemented with the concentrate supplement which contained the steam pressed barley. The experiment lasted for 60 days. The results showed as follows:1) there were no significant differences on the cecal microflora alpha diversity indexes of weaned foals among all groups (P>0.05). 2) The Bacteroidetes relative abundance of trial group Ⅲ was significantly higher than that of trial group Ⅰ and trial group Ⅱ (P<0.01), the Firmicutes relative abundance of trial group Ⅱ was significantly higher than that of trial group Ⅲ (P<0.01), the Actinobacteria relative abundance of trial group Ⅰ was significantly higher than that of trial group Ⅱ and trial group Ⅲ (P<0.01), and the Cyanobacteria relative abundance of trial group Ⅰ was significantly higher than that of trial group Ⅱ and trial group Ⅲ (P<0.05). 3) The Lactobacillaceae relative abundance of trial group Ⅱ was significantly higher than that of trial group Ⅲ (P<0.01), the Prevotellaceae relative abundance of trial group Ⅱ was significantly higher than that of trial group Ⅰ (P<0.05), the Lachnospiraceae relative abundance of trial group Ⅲ was significantly higher than that of trial group Ⅰ and trial group Ⅱ (P<0.05), and the Streptococcaceae relative abundance of trial group Ⅰ was significantly higher than that of trial group Ⅲ (P<0.01). 4) The Lactobacillus relative abundance of trial group Ⅱ was significantly higher than that of trial group Ⅲ (P<0.01), the Streptococcus relative abundance of trial group Ⅰ was significantly higher than that of trial group Ⅲ (P<0.05), the Sarcina relative abundance of trial group Ⅱ was significantly higher than that of trial group Ⅲ (P<0.05), the Alloprevotella relative abundance of trial group Ⅲ was significantly higher than that of trial group Ⅰ (P<0.01), and the Lactococcus relative abundance of trial group Ⅰ was significantly higher than that of trial group Ⅲ (P<0.01). 5) LEfSe analysis showed that trial group Ⅱ had 6 significant species and trial group Ⅲ had 3 significant species. The FAPROTAX function predicted 35 functions. In conclusion, under this experimental conditions, the weaned foals supplemented with barley can increase the relative abundances of Bacteroides and Firmicutes in cecum, decrease the relative abundances of Actinomycetes and Cyanobacteria, and promote the anaerobic respiration and acetic acid production of cecal microflora.

Cite this article

LI Qian , HUANG Xinxin , ZHANG Wenjie , TANGNAER· , Tuoliubieke , MA Chen , ZANG Changjia , CHEN Kaixu , LI Xiaobin . Effects of Different Cereal Starches on Cecal Microflora Diversity of Weaned Foals[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 488 -499 . DOI: 10.3969/j.issn.1006-267x.2022.01.046

References

[1] 黄菊,尹富贵,柏美娟,等.半纯合日粮法测定几种麦类及红薯淀粉在生长猪体内的消化率[J].农业现代化研究,2009,30(4):501-504. HUANG J,YIN F G,BAI M J,et al.Study on digestibility of starch from oat,wheat,buckwheat and sweet potato in intestinal tract of growing pigs by feeding semi-single diet[J].Research of Agricultural Modernization,2009,30(4):501-504.(in Chinese)
[2] 李岫怡,杨桂芹,郭东新.不同来源淀粉的组成、结构及其在动物生产上的应用研究进展[J].动物营养学报,2019,31(3):1053-1060. LI X Y,YANG G Q,GUO D X.Research progress on composition,structure and application of different starch sources in animal production[J].Chinese Journal of Animal Nutrition,2019,31(3):1053-1060.(in Chinese)
[3] 中国饲料数据库.中国饲料成分及营养价值表(2018年第29版)[J].中国饲料,2018(21):64-73. China Feed Database.Tables of feed composition and nutritive values in China (2018 twenty-ninth edition)[J].China Feed,2018(21):64-73.(in Chinese)
[4] WHELAN J,FRITSCHE K.Linoleic acid[J].Advances in Nutrition,2013,4(3):311-312.  
[5] 郭丽娜.燕麦品种品质及其降血脂功效研究[D].硕士学位论文.北京:中国农业科学院,2014. GUO L N.Quality and cholesterol-lowering effects of oat varieties[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences,2014.(in Chinese)
[6] 章海燕,张晖,王立,等.燕麦研究进展[J].粮食与油脂,2009(8):7-9. ZHANG H Y,ZHANG H,WANG L,et al.Research and development of oat[J].Cereals & Oils,2009(8):7-9.(in Chinese)
[7] 夏明亮,邹仕庚,程璐,等.大麦营养价值及其在猪生产中的合理使用[J].粮食与饲料工业,2015(8):57-60. XIA M L,ZOU S G,CHENG L,et al.The nutritional value of barley and its rational use in pig production[J].Cereal & Feed Industry,2015(8):57-60.(in Chinese)
[8] 王蕾,王悦,严宗山,等.大麦籽粒淀粉和β-葡聚糖积累特性研究[J].作物杂志,2020(2):119-124. WANG L,WANG Y,YAN Z S,et al.The research of starch and β-glucan accumulating characteristics in grain of different barley varieties[J].Crops,2020(2):119-124.(in Chinese)
[9] 赵金标,宋孝明,李忠超,等.不同的淀粉类型对生长猪饲粮有效能和营养物质消化率的影响[J].动物营养学报,2021,33(3):1330-1338. ZHAO J B,SONG X M,LI Z C,et al.Effects of different starch types on available energy and nutrient digestibility in growing pigs[J].Chinese Journal of Animal Nutrition,2021,33(3):1330-1338.(in Chinese)
[10] SUN Y,SU Y,ZHU W Y.Microbiome-metabolome responses in the cecum and colon of pig to a high resistant starch diet[J].Frontiers in Microbiology,2016,7:779.
[11] 刘军.淀粉对育肥猪生长性能和营养物质消化率的影响[J].当代畜禽养殖业,2017(12):8. LIU J.Effects of starch on the growth performance and nutrient digestibility of fattening pigs[J].Modern Livestock and Poultry Breeding Industry,2017(12):8.(in Chinese)
[12] WEURDING R E,VELDMAN A,VEEN W A,et al.Starch digestion rate in the small intestine of broiler chickens differs among feedstuffs[J].The Journal of Nutrition,2001,131(9):2329-2335.  
[13] YANG Y X,DAI Z L,ZHU W Y.Important impacts of intestinal bacteria on utilization of dietary amino acids in pigs[J].Amino Acids,2014,46(11):2489-2501.  
[14] 李晓斌,赵国栋,刘振,等.3-6月龄伊犁马肠道微生物群落多样性的研究[J].动物营养学报,2017,29(5):1535-1544. LI X B,ZHAO G D,LIU Z,et al.A study on intestinal microbiota diversity of 3-to 6-month-old Yili horses[J].Chinese Journal of Animal Nutrition,2017,29(5):1535-1544.(in Chinese)
[15] 李晓斌,李海,李凤鸣,等.断奶前纯血马马驹粪便菌群多样性分析[J].动物营养学报,2019,31(9):4363-4370. LI X B,LI H,LI F M,et al.Analysis of fecal microflora diversity for thoroughbred foals before weaning[J].Chinese Journal of Animal Nutrition,2019,31(9):4363-4370.(in Chinese)
[16] 刁慧,严鸿林,肖熠,等.不同品种猪肠道菌群结构和肠道发育模式的差异及其通过粪便移植在无菌小鼠上的传递性[C]//中国畜牧兽医学会动物营养学分会第十二次动物营养学术研讨会论文集,武汉:中国畜牧兽医学会,2016:272. DIAO H,YAN H L,XIAO Y,et al.Differences in the structure and intestinal development patterns of pig intestinal flora of different species and their transmission in sterile mice through fecal transplantation[C]//Proceedings of the 12th symposium on animal nutrition,Branch of Animal Nutrition,Chinese Society of Animal Husbandry and Veterinary Medicine,Wuhan:Chinese Association of Animal Science and Veterinary Medicine,2016:272.(in Chinese)
[17] CAMPBELL J H,FOSTER C M,VISHNIVETSKAYA T,et al.Host genetic and environmental effects on mouse intestinal microbiota[J].The ISME Journal,2012,6(11):2033-2044.  
[18] HUFELDT M R,NIELSEN D S,VOGENSEN F K,et al.Variation in the gut microbiota of laboratory mice is related to both genetic and environmental factors[J].Comparative Medicine,2010,60(5):336-347.
[19] 相振田.饲粮不同来源淀粉对断奶仔猪肠道功能和健康的影响及机理研究[D].博士学位论文.雅安:四川农业大学,2011. XIANG Z T.Effects of different dietary starches on intestinal functions and health and the underlying mechanism in weaned piglets[D].Ph.D.Thesis.Ya'an:Sichuan Agricultural University,2011.(in Chinese)
[20] MARTINEZ I,LATTIMER J M,HUBACH K L,et al.Gut microbiome composition is linked to whole grain-induced immunological improvements[J].ISME Journal,2013,7(2):269-280.  
[21] MAKKI K,DEEHAN E C,WALTER J,et al.The impact of dietary fiber on gut microbiota in host health and disease[J].Cell Host & Microbe,2018,23(6):705-715.  
[22] 张小凤,郭雁君,蒋惠,等.动物肠道屏障功能对肠道菌群变化的调控[J].湖北农业科学,2018,57(12):5-8,32. ZHANG X F,GUO Y J,JIANG H,et al.The regulation of intestinal barrier function on intestinal flora changes in animals[J].Hubei Agricultural Sciences,2018,57(12):5-8,32.(in Chinese)
[23] 王珊珊,王佳堃,刘建新.肠道微生物对宿主免疫系统的调节及其可能机制[J].动物营养学报,2015,27(2):375-382. WANG S S,WANG J K,LIU J X.Regulation of host immune system by gut microbiota and its possible mechanisms[J].Chinese Journal of Animal Nutrition,2015,27(2):375-382.(in Chinese)
[24] KOCHERGINSKAYA S A,AMINOV R I,WHITE B A.Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis,random sequencing,and statistical ecology approaches[J].Anaerobe,2001,7(3):119-134.  
[25] TAJIMA K,ARAI S,OGATA K,et al.Rumen bacterial community transition during adaptation to high-grain diet[J].Anaerobe,2000,6(5):273-284.  
[26] ZHOU M,HERNANDEZ-SANABRIA E,GUAN L L.Characterization of variation in rumen methanogenic communities under different dietary and host feed efficiency conditions,as determined by PCR-denaturing gradient gel electrophoresis analysis[J].Applied and Environmental Microbiology,2010,76(12):3776-3786.  
[27] 叶翔杨,宫瑞光,任战军.饮水中添加复合维生素纳米乳对生长獭兔生长性能、免疫功能、抗氧化能力、肠道组织结构及盲肠微生态的影响[J].动物营养学报,2021,33(2):1044-1054. YE X Y,GONG R G,REN Z J.Effects of multivitamin nanoemulsion on growth performance,immune function,antioxidant ability,intestinal tissue structure and cecal microecology of growing Rex rabbits[J].Chinese Journal of Animal Nutrition,2021,33(2):1044-1054.(in Chinese)
[28] 王璇,王丹丹,年芳,等.不同淀粉源饲粮对肉仔鸡小肠消化酶活性、盲肠挥发性脂肪酸含量和微生物菌群的影响[J].动物营养学报,2021,33(1):285-296. WANG X,WANG D D,NIAN F,et al.Effects of different starch source diets on small intestinal digestive enzyme activities,caecum volatile fatty acid contents and microbial community of broilers[J].Chinese Journal of Animal Nutrition,2021,33(1):285-296.(in Chinese)
[29] FLINT H J,BAYER E A,RINCON M T,et al.Polysaccharide utilization by gut bacteria:potential for new insights from genomic analysis[J].Nature Reviews Microbiology,2008,6(2):121-131.  
[30] GONG L X,CAO W Y,CHI H L,et al.Whole cereal grains and potential health effects:involvement of the gut microbiota[J].Food Research International,2018,103:84-102.
[31] 肖瑜,杨爽,刘炳利,等.影响糯性谷物淀粉的消化因素及其改性方法[J].食品工业,2019,40(4):263-268. XIAO Y,YANG S,LIU B L,et al.The factors of digestibility on waxy starch and the modified method[J].The Food Industry,2019,40(4):263-268.(in Chinese)
[32] EVANS N J,BROWN J M,MURRAY R D,et al.Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes[J].Applied and Environmental Microbiology,2011,77(1):138-147.  
[33] 宾石玉,赵霞.日粮淀粉来源对断奶仔猪小肠淀粉消化率的影响[J].贺州学院学报,2007,23(1):141-144. BIN S Y,ZHAO X.The effect of dietary starch source on starch digestion of small intestine in weaned pigs[J].Journal of Hezhou University,2007,23(1):141-144.(in Chinese)
[34] 黄伟,杨秀娟,曹志勇,等.日粮淀粉直支比对断奶仔猪养分消化率和生长性能的影响[J].中国饲料,2017(14):20-23. HUANG W,YANG X J,CAO Z Y,et al.Effect of dietary starch amylose ratio on nutrient digestibility and growth performance of weaned piglets[J].China Feed,2017(14):20-23.(in Chinese)
[35] YOUNG V B.The intestinal microbiota in health and disease[J].Current Opinion in Gastroenterology,2012,28(1):63-69.  
[36] 孙玲利,宁俊平,韩战强,等.家禽肠道菌群与营养物质代谢的研究进展[J].黑龙江畜牧兽医,2021(3):54-57. SUN L L,NING J P,HAN Z Q,et al.Research progress of poultry gut microflora and nutrient metabolism[J].Heilongjiang Animal Science and Veterinary Medicine,2021(3):54-57.(in Chinese)
[37] WANG W,CHEN L P,ZHOU R,et al.Increased proportions of Bifidobacterium and the Lactobacillus group and loss of butyrate-producing bacteria in inflammatory bowel disease[J].Journal of Clinical Microbiology,2014,52(2):398-406.  
[38] HAN G Q,XIANG Z T,YU B,et al.Effects of different starch sources on Bacillus spp. in intestinal tract and expression of intestinal development related genes of weanling piglets[J].Molecular Biology Reports,2012,39(2):1869-1876.  
[39] 王凤梅,樊明寿,郑克宽.燕麦β-葡聚糖的保健作用及影响其积累的因素[J].麦类作物学报,2005,25(2):116-118. WANG F M,FAN M S,ZHENG K K.Nutritive value of oat β-glucan and the factors affecting its accumulation[J].Journal of Triticeae Crops,2005,25(2):116-118.(in Chinese)
[40] 张斌,罗发兴,黄强,等.不同直链含量玉米淀粉结晶结构及其消化性研究[J].食品与发酵工业,2010,36(8):26-30. ZHANG B,LUO F X,HUANG Q,et al.Crystalline structures and digestibility of cornstarches with different amylose/amylopectin content[J].Food and Fermentation Industries,2010,36(8):26-30.(in Chinese)
[41] TOPPING D L,CLIFTON P M.Short-chain fatty acids and human colonic function:roles of resistant starch and nonstarch polysaccharides[J].Physiological Reviews,2001,81(3):1031-1064.  
[42] KOH A,DE VADDER F,KOVATCHEVA-DATCHARY P,et al.From dietary fiber to host physiology:short-chain fatty acids as key bacterial metabolites[J].Cell,2016,165(6):1332-1345.  
[43] 余群莲.不同来源淀粉对肉牛瘤胃发酵及营养物质消化代谢的影响[D].硕士学位论文.雅安:四川农业大学,2011. YU Q L.Effect of different sources of starch on rumen fermentation,digestion and metabolism of nutrients in beef cattle[D].Master's Thesis.Ya'an:Sichuan Agricultural University,2011.(in Chinese)
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