Effects of Dietary Vitamin K3 Content on Digestive Ability, Serum Metabolism Indexes, Muscle Amino Acid and Fatty Acid Composition of Largemouth Bass (Micropterus salmoides)

WEI Xiang; YAN Ketao; DAI Yufeng; HUA Xueming; LI Xiang; HANG Ying

doi:10.3969/j.issn.1006-267x.2022.01.049

2022 , Vol. 34 >Issue 1: 533 - 543

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.049

RESEARCH PAPER

Effects of Dietary Vitamin K₃ Content on Digestive Ability, Serum Metabolism Indexes, Muscle Amino Acid and Fatty Acid Composition of Largemouth Bass (Micropterus salmoides)

WEI Xiang ,
YAN Ketao ,
DAI Yufeng ,
HUA Xueming ,
LI Xiang ,
HANG Ying

Expand

1. Centre for Research on Environmental Ecology and Fish Nutrition of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;
2. Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;
3. National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China;
4. Administration Bureau of Wangwu Revervoir, Longkou 265721, China;
5. Zhenglu Animal Husbandry and Veterinary Station, Changzhou 213111, China

Received date: 2021-05-06

Online published: 2022-01-18

Fold

Abstract

An 8-week feeding trail was carried out to investigate the effects of dietary vitamin K₃ content on the digestive ability, serum metabolism indexes, muscle amino acid and fatty acid composition of largemouth bass (Micropterus salmoides). vitamin K₃ was supplemented into a basal diet with the levels of 0.78 (K0 group), 5.80 (K5 group), 10.82 (K10 group), 15.84 (K15 group) and 20.85 mg/kg (K20 group), and then the five experimental diets were fed to healthy juvenile largemouth bass with the initial average body weight of (12.96±0.07) g. The results showed that the activities of protease, amylase and lipase in the stomach and intestine of the groups with vitamin K₃ supplementation were significantly lower than those of K0 group (P<0.05). The apparent digestibility and protein apparent digestibility of K10, K15 and K20 groups were significantly lower than those of K0 group (P<0.05). The serum urea nitrogen content of K20 group was significantly higher than that in the other four groups (P<0.05), but there were no significant differences in the serum total protein, albumin, total cholesterol and triglyceride contents among the five groups (P>0.05). There were no significant differences in the contents of muscle essential amino acids, non-essential amino acids and total amino acids among all groups (P>0.05), essential amino acid index as well, but with the increase of dietary vitamin K₃ content, the contents of essential amino acids and total amino acids showed an increasing trend. The content of muscle monounsaturated fatty acids of K15 group was significantly higher than that of other four groups (P<0.05), and the contents of C16:1n7 and C18:1n5 were significantly increased compared with other groups (P<0.05). There were no significant differences in the contents of muscle saturated fatty acids and polyunsaturated fatty acids among all groups (P>0.05). In summary, dietary supplementation of vitamin K₃ can improve the muscle amino acid composition, but it decreases the digestive ability when the vitamin K₃ content is equal or greater than 5.80 mg/kg, and high dose of dietary vitamin K₃ changes protein and fatty acid metabolism.

Key words： largemouth bass; vitamin K₃; digestion; metabolism; muscle composition

Cite this article

WEI Xiang , YAN Ketao , DAI Yufeng , HUA Xueming , LI Xiang , HANG Ying . Effects of Dietary Vitamin K₃ Content on Digestive Ability, Serum Metabolism Indexes, Muscle Amino Acid and Fatty Acid Composition of Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 533 -543 . DOI: 10.3969/j.issn.1006-267x.2022.01.049

References

[1] BOOTH S L.Roles for vitamin K beyond coagulation[J].Annual Review of Nutrition,2009,29:89-110.
[2] FERLAND G.The vitamin K-dependent proteins:an update[J].Nutrition Reviews,1998,56(8):223-230.
[3] VERMEER C,JIE K S,KNAPEN M H.Role of vitamin K in bone metabolism[J].Annual Review of Nutrition,1995,15:1-22.
[4] SHEARER M J.Vitamin K[J].The Lancet,1995,345(8944):229-234.

[5] STAFFORD D W.The vitamin K cycle[J].Journal of Thrombosis and Haemostasis,2005,3(8):1873-1878.

[6] GROFF J L,GROPPER S S.Vitamin K[M]//GROFF J L,GROPPER S S.Advanced nutrition and human metabolism.3rd ed.[S.l.]:Wadsworth,1999:351-358.
[7] UDAGAWA M,NAKAZOE J I,MURAI T.Tissue distribution of phylloquinone and menaquinone-4 in sardine,Sardinops melanostictus[J].Comparative Biochemistry and Physiology Part B:Comparative Biochemistry,1993,106(2):297-301.

[8] GRAHL-MADSEN E,LIE Ø.Effects of different levels of vitamin K in diets for cod (Gadus morhua)[J].Aquaculture,1997,151(1/2/3/4):269-274.
[9] HALVER J E.The vitamins[M]//Fish nutrition.New York:Academic Press,1989:31-109.
[10] POSTON H A.Relative effect of two dietary water-soluble analogues of menaquinone on coagulation and packed cell volume of blood of lake trout (Salvelinus namaycush)[J].Journal of the Fisheries Research Board of Canada,1976,33(8):1791-1793.

[11] TAVEEKIJAKARN P,MIYAZAKI T,MATSUMOTO M,et al.Studies on vitamin K deficiency in amago salmon,Oncorhynchus rhodurus (Jordan & McGregor)[J].Journal of Fish Diseases,1996,19(13):209-214.
[12] UDAGAWA M.The effect of dietary vitamin K (phylloquinone and menadione) levels on the vertebral formation in mummichog Fundulus heteroclitus[J].Fisheries Science,2001,67(1):104-109.

[13] 蒋明,王卫民,文华,等.维生素K₃对草鱼生长、体成分和凝血时间的影响[J].淡水渔业,2007,37(2):61-64. JIANG M,WANG W M,WEN H,et al.Effects of dietary vitamin K₃ on growth,carcass composition and blood coagulation time for grass carp fingerling (Ctenopharyngodon idellus)[J].Freshwater Fisheries,2007,37(2):61-64.(in Chinese)
[14] 段元慧,朱晓鸣,韩冬,等.异育银鲫幼鱼对饲料中维生素K需求的研究[J].水生生物学报,2013,37(1):8-15. DUAN Y H,ZHU X M,HAN D,et al.Dietary vitamin K requirement of juvenile gibel carp (Carassius auratus gibelio)[J].Acta Hydrobiologica Sinica,2013,37(1):8-15.(in Chinese)
[15] 李贞仪.吴郭鱼稚鱼与石斑鱼稚鱼对维生素K最适需求量之探讨[D].硕士学位论文.基隆:台湾海洋大学,2003. LI Z Y.Vitamin K requirements of juvenile hybrid tilapia (Oreochromis niloticus×O. aureus) and grouper (Epinephelus malabaricus)[D].Master's Thesis.Keelung City:National Taiwan Ocean University,2003.(in Chinese)
[16] CHENG L,ZHANG W B,LIN S Q,et al.Effects of dietary vitamin K on growth performances,blood coagulation time and menaquinone-4(MK-4) concentration in tissues of juvenile large yellow croaker Pseudosciaena crocea[J].Aquaculture Research,2015,46(5):1269-1275.

[17] SHIAU S Y,LIU J S.Estimation of the dietary vitamin K requirement of juvenile Penaeus chinensis using menadione[J].Aquaculture,1994,126(1/2):129-135.
[18] SHIAU S Y,LIU J S.Quantifying the vitamin K requirement of juvenile marine shrimp (Penaeus monodon) with menadione[J].Journal of Nutrition,1994,124(2):277-282.

[19] MURAI T,ANDREWS J W.Vitamin K and anticoagulant relationships in catfish diets[J].Bulletin of the Japanese Society of Scientific Fisheries,1977,43(7):785-794.

[20] SHIMENOS.Yellowtail,Seriola quinqueradiata[M].Handbook of Nutrient Requirements of Finfish,[S.l.]:[s.n.]1991:181-191.
[21] KROSSØY C,WAAGBØ R,FJELLDAL P G,et al.Dietary menadione nicotinamide bisulphite (vitamin K₃) does not affect growth or bone health in first-feeding fry of Atlantic salmon (Salmo salar L.)[J].Aquaculture Nutrition,2009,15(6):638-649.

[22] YUAN J,FENG L,JIANG W D,et al.Effects of dietary vitamin K levels on growth performance, enzyme activities and antioxidant status in the hepatopancreas and intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture Nutrition,2016,22(2):352-366.

[23] 吕梅,王宝维,殷太岳,等.维生素K₃对五龙鹅生长性能、屠宰性能及养分表观利用率的影响[J].动物营养学报,2016,28(9):2733-2741. LYU M,WANG B W,YIN T Y,et al.Effects of vitamin K₃ on growth performance,slaughter performance and nutrient apparent availability of Wulong geese[J].Chinese Journal of Animal Nutrition,2016,28(9):2733-2741.(in Chinese)
[24] 王洋,徐奇友,王常安.维生素K₃对松浦镜鲤幼鱼血清生化指标的影响[J].吉林农业大学学报,2013,35(4):490-495. WANG Y,XU Q Y,WANG C A.Effects of vitamin K₃ on serum biochemical indexes of fingerling Song-pu mirror carp[J].Journal of Jilin Agricultural University,2013,35(4):490-495.(in Chinese)
[25] 农业农村部渔业渔政管理局,全国水产技术推广总站,中国水产学会.中国渔业统计年鉴2020[M].北京:中国农业出版社,2020. Fishery Administration Bureau of Ministry of Agriculture and Rural Areas,National Fisheries Technology Extension Center,China Society of Fisheries.China fishery statistical yearbook 2020[M].Beijing:China Agriculture Press,2020.(in Chinese)
[26] ANDERSON R J,KIENHOLZ E W,FLICKINGER S A.Protein requirements of smallmouth bass and largemouth bass[J].The Journal of Nutrition,1981,111(6):1085-1097.

[27] BRIGHT L A,COYLE S D,TIDWELL J H.Effect of dietary lipid level and protein energy ratio on growth and body composition of largemouth bass Micropterus salmoides[J].Journal of the World Aquaculture Society,2005,36(1):129-134.
[28] 陈乃松,马建忠,周恒永,等.大口黑鲈对饲料中蛋氨酸需求量的评定[J].水产学报,2010,34(8):1244-1253. CHEN N S,MA J Z,ZHOU H Y,et al.Assessment of dietary methionine requirement in largemouth bass,Micropterus salmoides[J].Journal of Fisheries of China,2010,34(8):1244-1253.(in Chinese)
[29] CHEN Y J,YUAN R M,LIU Y J,et al.Dietary vitamin C requirement and its effects on tissue antioxidant capacity of juvenile largemouth bass,Micropterus salmoides[J].Aquaculture,2015,435:431-436.
[30] DAIRIKI J K,DOS SANTOS DIAS C T,CYRINO J E P.Lysine requirements of largemouth bass,Micropterus salmoides:a comparison of methods of analysis of dose-response trials data[J].Journal of Applied Aquaculture,2007,19(4):1-27.

[31] LI S L,LIAN X Y,CHEN N S,et al.Effects of dietary vitamin E level on growth performance, feed utilization,antioxidant capacity and nonspecific immunity of largemouth bass,Micropterus salmoides[J].Aquaculture Nutrition,2018,24(6):1679-1688.

[32] 连雪原,陈乃松,王孟乐,等.大口黑鲈维生素A需求量[J].动物营养学报,2017,29(10):3819-3830. LIAN X Y,CHEN N S,WANG M L,et al.Dietary vitamin a requirement of largemouth bass (Micropterus salmoides)[J].Chinese Journal of Animal Nutrition,2017,29(10):3819-3830.(in Chinese)
[33] ZHOU H,CHEN N,QIU X,et al.Arginine requirement and effect of arginine intake on immunity in largemouth bass,Micropterus salmoides[J].Aquaculture Nutrition,2012,18(1):107-116.

[34] ZHU Y,CHEN Y J,LIU Y J,et al.Effect of dietary selenium level on growth performance, body composition and hepatic glutathione peroxidase activities of largemouth bass Micropterus salmoide[J].Aquaculture Research,2012,43(11):1660-1668.

[35] WEI X,HANG Y,LI X,et al.Effects of dietary vitamin K₃ levels on growth,coagulation,calcium content,and antioxidant capacity in largemouth bass,Micropterus salmoides[J].Aquaculture and Fisheries,2021,https://doi.org/10.1016/j.aaf.2021.08.004.
[36] 木晓云,董跃伟,温晓江,等.反相高效液相色谱法测定维生素K₂软胶囊中K₂(20)含量[J].分析试验室,2009,28(增刊1):33-35. MU X Y,DONG Y W,WEN X J,et al.The content of vitamin K₂ (20) in vitamin K₂ soft capsule was determined by RP-HPLC[J].Chinese Journal of Analysis Laboratory,2009,28(S1):33-35.(in Chinese)
[37] 杨月欣,中国疾病预防控制中心营养与健康所.中国食物成分表[M].6版.北京:北京大学医学出版社,2019:218-219. YANG Y X,National Institute for Nutrition and Health Chinese Center for Disease Control and Prevention.China food composition tables[M].6th ed.Beijing:Peking University Medical Press,2019:218-219.(in Chinese)
[38] GARCÍA-GASCA A,GALAVIZ M A,GUTIÉRREZ J N,et al.Development of the digestive tract,trypsin activity and gene expression in eggs and larvae of the bullseye puffer fish Sphoeroides annulatus[J].Aquaculture,2006,251(2/4):366-376.
[39] LIN Y,XIAO Q Z.Dietary glutamine supplementation improves structure and function of intestine of juvenile Jian carp (Cyprinus carpio var. Jian)[J].Aquaculture,2006,256(1/4):389-394.
[40] 刘朝明,吴彩霞,李和平.维生素K在畜牧业中的应用[J].农家科技,2011(7):40. LIU Z M,WU C X,LI H P.Application of vitamin K in animal husbandry[J].Farmer science and technology,2011(7):40.(in Chinese)
[41] EINARSSON S,DAVIES P S,TALBOT C.Effect of exogenous cholecystokinin on the discharge of the gallbladder and the secretion of trypsin and chymotrypsin from the pancreas of the Atlantic salmon,Salmo salar L[J].Comparative Biochemistry and Physiology, Part C:Pharmacology,Toxicology & Endocrinology,1997,117(1):63-67.

[42] 刘艳丰,唐淑珍,张文举,等.沙棘叶黄酮对阿勒泰羊生长性能、屠宰性能和血清生化指标的影响[J].畜牧兽医学报,2014,45(12):1981-1987. LIU Y F,TANG S Z,ZHANG W J,et al.Effects of flavonoids of sea buckthorn leaves on growth performance,slaughter performance and serum biochemical indexes of Altay sheep[J].Acta Veterinaria et Zootechnica Sinica,2014,45(12):1981-1987.(in Chinese)
[43] 占今舜,杨群,钟小军,等.不同精粗比饲粮对湖羊肉品质、血液指标和肠道发育的影响[J].草业科学,2019,36(12):3166-3174. ZHAN J S,YANG Q,ZHONG X J,et al.Effects of total mixed rations with different concentration-roughage ratios on meat quality,serum indices,and intestinal tract development in Hu sheep[J].Pratacultural Science,2019,36(12):3166-3174.(in Chinese)
[44] 罗云.联合检测血清总胆汁酸和前清蛋白在肝脏疾病诊断中的应用[J].重庆医学,2007,36(12):1167-1168,1170. LUO Y.Application of combined detection of serum total bile acids and prealbumin in diagnosis of liver diseases[J].Chongqing Medicine,2007,36(12):1167-1168,1170.(in Chinese)
[45] CHIOU T J,ZHANG J,FERRANS V J,et al.Cardiac and renal toxicity of menadione in rat[J].Toxicology,1997,124(3):193-202.

[46] REBHUN W C,TENNANT B C,DILL S G,et al.Vitamin K₃-induced renal toxicosis in the horse[J].Journal of the American Veterinary Medical Association,1984,184(10):1237-1239.
[47] 王小生.必需氨基酸对人体健康的影响[J].中国食物与营养,2005(7):48-49. WANG X S.Effects of essential amino acids in food on human body health[J].Food and Nutrition in China,2005(7):48-49.(in Chinese)
[48] 冯东勋.利用必需氨基酸指数(EAAI)评价新饲料蛋白源[J].中国饲料,1997(7):10-13. FENG D X.The essential amino acid index (EAAI) was used to evaluate the protein source of neologous compounds[J].China Feed,1997(7):10-13.(in Chinese)
[49] ÇELIK M,DILER A,KVÇVKGVLMEZ A.A comparison of the proximate compositions and fatty acid profiles of zander (Sander lucioperca) from two different regions and climatic conditions[J].Food Chemistry,2005,92(4):637-641.

[50] 夏琛,崔心禹,项婷,等.棕榈油酸功能的研究进展[J].中国油脂,2020,45(2):39-43. XIA C,CUI X Y,XIANG T,et al.Progress in the function of palmitoleic acid[J].China Oils and Fats,2020,45(2):39-43.(in Chinese)
[51] 陈哲,朱帅伟,马立威,等.反式油酸对H9c2心肌细胞增殖抑制及诱导凋亡作用的研究[J].中国药学杂志,2020,55(3):199-205. CHEN Z,ZHU S W,MA L W,et al.Effect of trans-oleic acid on proliferation inhibition and apoptosis induction in H9c2 cardiomyocyte[J].Chinese Pharmaceutical Journal,2020,55(3):199-205.(in Chinese)
[52] 李昌伟,韦君,韦玲静,等.投喂蚕豆对官垌草鱼生长性能和肌肉氨基酸、脂肪酸含量的影响[J].广西畜牧兽医,2019,35(2):51-54. LI C W,WEI J,WEI L J,et al.Effects of broad bean feeding on growth performance and muscle amino acid and fatty acid contents of Guandong grass carp[J].Guangxi Journal of Animal Husbandry & Veterinary Medicine,2019,35(2):51-54.(in Chinese)
[53] 李乃顺,冷向军,李小勤,等.绿原酸对草鱼鱼种生长、非特异性免疫和肉质的影响[J].水生生物学报,2014,38(4):619-626. LI N S,LENG X J,LI X Q,et al.The effects of chlorogenic acid on the growth,non-specific immune index and the meat quality of juvenile grass carp (Ctenopharyngodon idellus)[J].Acta Hydrobiologica Sinica,2014,38(4):619-626.(in Chinese)
[54] 冷向军,孟晓林,李家乐,等.杜仲叶对草鱼生长、血清非特异性免疫指标和肉质影响的初步研究[J].水产学报,2008,32(3):434-440. LENG X J,MENG X L,LI J L,et al.Effect of Du-zhong (Eucommia ulmoides Oliver) leaf on growth,serum non-specific immune index and meat quality of grass carp[J].Journal of Fisheries of China,2008,32(3):434-440.(in Chinese)

Options

Outlines

模态框（Modal）标题

Abstract

Cite this article

References