RESEARCH PAPER

Protective Effects of Bamboo Leaf Flavonoids on Oxidative Damage of Bovine Mammary Epithelial Cells Induced by Heat Stress

  • LI Jianfeng ,
  • ZHAO Jiazhen ,
  • SHEN Yiyuan ,
  • GUAN Shuwen ,
  • HAN Siyu ,
  • XIONG Benhai ,
  • WANG Meng ,
  • CHU Kangkang ,
  • FANG Luoyun ,
  • TONG Jinjin ,
  • JIANG Linshu
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  • 1. Beijing Key Laboratory of Dairy Nutrition, School of Animal Science and Technology, Beijing Institute of Agriculture, Beijing 102206, China;
    2. Beijing Institute of Animal Husbandry and Veterinary Medicine, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Beijing Capital Agribusiness Group, Beijing 100192, China

Received date: 2021-06-23

  Online published: 2022-01-18

Abstract

The objective of this study was to investigate the protective effects of bamboo leaf flavonoid (BLF) on bovine mammary epithelial cells (BMECs) response to heat stress. Experiments used BMECs treated without BLF, 37℃ as the control group and BMECs treated without BLF, 42℃ as the heat stress group, with different concentrations of BLF (1, 5, 10 g/mL), 42℃ treated BMECs as the experimental groups, with 3 replicates in each group. Lactate dehydrogenase (LDH) method was used to detect the effects of BLF on oxidative damage of BMECs induced by heat stress, as well as the reactive oxygen species (ROS) content, mitochondrial membrane potential (MMP) level and antioxidant indexes were detected. Quantitative real-time PCR was used to detect the gene expression related to apoptosis and nuclear factor erythroid 2 related factor 2 (Nrf2) signaling pathway. The results showed as follows:compared with the control group, BMECs activity decreased significantly in heat stress group (P<0.05). While the concentrations of 1、5、10 μg/mL BLF could significantly reduce the early apoptosis rate and total apoptosis rate and MMP level of BMECs (P<0.01), and the content of intracellular ROS significantly inhibited in BMECs (P<0.01). Moreover, compared with heat stress group, BLF significantly increased the activity of total superoxide disambiguase (T-SOD) and glutathione peroxidase (GSH-Px) while decreasing the content of malondialdehyde (MDA) (P<0.01). Real-time PCR showed that BLF significantly decreased the mRNA relative expression levels of heat shock transcription factor 1 (HSF1), heat shock protein 70 (HSP70), heat shock protein 90 (HSP90), cyclooxygenase-2 (COX-2) and cysteine protease-3 (Caspase-3) (P<0.01), and the mRNA relative expression levels of Nrf2, heme oxygenase-1 (HO-1), cysteine uptake transporter (xCT), thioredoxin reduction 1 (Txnrd1) and NADPH quinone oxidoreductase 1 (NQO1) significantly up-regulated compared with heat stress group (P<0.01). In conclusion, BLF alleviates the oxidative damage of BMECs induced by heat stress by regulating key genes in Nrf2 signaling pathway, and 5 μg/mL BLF has the best protective effect on the oxidative damage of BMECs induced by heat stress.

Cite this article

LI Jianfeng , ZHAO Jiazhen , SHEN Yiyuan , GUAN Shuwen , HAN Siyu , XIONG Benhai , WANG Meng , CHU Kangkang , FANG Luoyun , TONG Jinjin , JIANG Linshu . Protective Effects of Bamboo Leaf Flavonoids on Oxidative Damage of Bovine Mammary Epithelial Cells Induced by Heat Stress[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 626 -639 . DOI: 10.3969/j.issn.1006-267x.2022.01.057

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