Effects of Fermented Wheat Bran Polysaccharides on Microbiota and Rumen Barrier Junction Related Indices of Dorper&times;Thin-Tailed Han Crossbred Meat Lambs

WANG Wenwen; WANG Yuan; MENG Ziqi; AN Xiaoping; QI Jingwei

doi:10.3969/j.issn.1006-267x.2022.01.058

2022 , Vol. 34 >Issue 1: 640 - 650

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.01.058

RESEARCH PAPER

Effects of Fermented Wheat Bran Polysaccharides on Microbiota and Rumen Barrier Junction Related Indices of Dorper×Thin-Tailed Han Crossbred Meat Lambs

WANG Wenwen ,
WANG Yuan ,
MENG Ziqi ,
AN Xiaoping ,
QI Jingwei

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1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Inner Mongolia Herbivorous Livestock Feed Engineering and Technology Research Center, Hohhot 010018, China;
3. Institute of Animal Nutrition and Feed in Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China

Received date: 2021-06-10

Online published: 2022-01-18

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Abstract

This study was conducted to investigate the effects of fermented wheat bran polysaccharides (FWBPs) on expression of tight junction protein and cytokine genes in rumen tissue and rumen microbiota structure of Dorper×thin-tailed Han crossbred meat lambs. Twenty-one 6-week-old Dorper×thin-tailed Han crossbred meat lambs with similar body weight[(21.85±0.64) kg] were randomly assigned into three groups of seven lambs in each group. Lambs in the three groups were fed basal diets supplemented with 0 (control group), 200 (low dose group) and 400 mg/kg BW (high dose group) FWBPs, respectively. The pre-experimental period lasted for 14 days, and the experimental period lasted for 56 days. The results showed as follows:1) compared with the control group, the mRNA relative expression levels of occludin, claudin-1 and claudin-4 in rumen tissue of meat lambs of low dose group were significantly increased (P<0.05), and the mRNA relative expression levels of occludin, claudin-1, claudin-4, zonula occludens-1 (ZO-1) interferon-γ (IFN-γ) and interleukin-6 (IL-6) in rumen tissue of meat lambs of high dose group were significantly increased (P<0.05). 2) The Shannon index and Simpson index in rumen microbiota of meat lambs of high dose group were significantly higher than those of the control and low dose group (P<0.01). Compared with the control group, the relative abundances of Spirochaetota and Succinivibrio in rumen microbiota of meat lambs of low dose group were significantly increased (P<0.05); the relative abundances of Proteobacteria, Succinivibrionaceae_UCG-001 and Succiniclasticum in rumen microbiota of meat lambs of high dose group were significantly decreased (P<0.05 or P<0.01), and the Bacteroidota and Selenomonas relative abundances were significantly increased (P<0.05). The relative abundances of Proteobacteria, Spirochaetota and Succinivibrionaceae_UCG-001 in rumen microbiota of meat lambs of high dose group were significantly lower than those of low dose group (P<0.05 or P<0.01), while the Bacteroidota, Veillonellaceae_UCG-001 and Selenomonas relative abundances were significantly higher than those of low dose group (P<0.01). 3) The linear discriminant analysis effect size (LEfSe) analysis showed that Erysipelotrichales, Acidaminococcaceae, Acidaminococcales and Succiniclasticum were enriched in the control group, the Succinivibrionaceae, Aeromonadales, Gammaproteobacteria, Proteobacteria and Succinivibrionaceae_UCG-001 were enriched in low dose group, and the Bacteroidota, Bacteroidia, Bacteroidales, Prevotellaceae, Selenomonadaceae, Prevotella_ruminicola, Selenomonas, Selenomonas_ruminantium and Veillonellaceae_UCG-001 were enriched in high dose group. In conclusion, FWBPs can increase tight junction protein and cytokine mRNA relative expression levels of Dorper×thin-tailed Han crossbred meat lambs, regulate the rumen microbiota structure, and maintain the rumen health.

Key words： fermented wheat bran polysaccharides; meat lambs; rumen microbiota structure; tight junction protein; cytokine

Cite this article

WANG Wenwen , WANG Yuan , MENG Ziqi , AN Xiaoping , QI Jingwei . Effects of Fermented Wheat Bran Polysaccharides on Microbiota and Rumen Barrier Junction Related Indices of Dorper×Thin-Tailed Han Crossbred Meat Lambs[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 640 -650 . DOI: 10.3969/j.issn.1006-267x.2022.01.058

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