RESEARCH PAPER

Effects of Sterol-Regulatory Element Binding Protein-1 Gene on Blood Fatty Acid Composition of Simmental Cattle Raised in Alpine Grassland

  • LI Xinmiao ,
  • WANG Yuanyuan ,
  • HESHUOTE Mailisi ,
  • BAI Yuting ,
  • Baoyindugurong· ,
  • Jinhua ,
  • Hugejiletu ,
  • Aorigele ,
  • XUE Qiang
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  • 1. College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China;
    2. Inner Mongolia Key Laboratory of Bio-Manufacturing, Hohhot 010018, China;
    3. Columbia University, New York 10027, USA;
    4. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China;
    5. Inner Mongolia Hesige Green Industry Import and Export Co., Ltd., Xilingol 026321, China

Received date: 2021-06-04

  Online published: 2022-01-18

Abstract

This experiment was conducted to study the effects of sterol-regulatory element binding protein-1 (SREBP-1) gene on blood fatty acid composition of Simmental cattle raised in alpine grassland, and to provide a theoretical basis for the preservation of excellent genetic genes in the future process of breeding. Blood samples were collected from thirty 18-month-old alpine grasslands raised Australian imported Simmental bulls. The composition and content of fatty acids in blood were studied by gas chromatography-mass spectrometry method, the relative expression level of SREBP-1 gene was determined by real-time fluorescence quantitative PCR, and then the SREBP-1 gene was sequenced. The results showed as that 36 kinds of fatty acids were detected in the blood of pure Simmental cattle raised in alpine steppe, and the palmitic acid (12.57%), stearic acid (21.76%), oleic acid (22.11%) and linoleic acid (29.53%) contents were higher. The blood SREBP-1 gene relative expression level was positively correlated with palmitic acid, palmitoleic acid, stearic acid, oleic acid and monounsaturated fatty acid (P>0.05). The blood SREBP-1 gene relative expression level had significant effects on myristic acid, myristic acid, palmitic acid, linoleic acid and saturated fatty acids (SFA), as well as monounsaturated fatty acids/saturated fatty acids (MUFA/SFA), C14 index, C16 index, C18 index and elongation index in blood fatty acids of Simmental cattle. The SREBP-1 gene had insertion and deletion polymorphism at 84 bp of intron 5, which was characterized LL and LS genotypes. The LL and LS genotypes of SREBP-1 gene had no significant effects no fatty acid composition (P>0.05). In conclusion, the expression of SREBP-1 gene in blood of pure Simmental cattle raised in alpine grassland plays an irreplaceable role in regulating fatty acids. Therefore, the SREBP-1 gene can be used as an important candidate gene for breeding and evaluation of Simmental cattle breeds raised in alpine steppe.

Cite this article

LI Xinmiao , WANG Yuanyuan , HESHUOTE Mailisi , BAI Yuting , Baoyindugurong· , Jinhua , Hugejiletu , Aorigele , XUE Qiang . Effects of Sterol-Regulatory Element Binding Protein-1 Gene on Blood Fatty Acid Composition of Simmental Cattle Raised in Alpine Grassland[J]. Chinese Journal of Animal Nutrition, 2022 , 34(1) : 651 -658 . DOI: 10.3969/j.issn.1006-267x.2022.01.059

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