Effects of Embedded Lactobacillus plantarum and Enterococcus faecium on Growth Performance, Immune and Antioxidant Function and Intestinal Microflora of Broilers

SONG Dan; DUAN Tao; CHEN Junlin; QIAO Lin; WANG Li; CHENG Lixian; LI Aike; WANG Weiwei

doi:10.3969/j.issn.1006-267x.2022.02.021

2022 , Vol. 34 >Issue 2: 877 - 887

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.021

RESEARCH PAPER

Effects of Embedded Lactobacillus plantarum and Enterococcus faecium on Growth Performance, Immune and Antioxidant Function and Intestinal Microflora of Broilers

SONG Dan ,
DUAN Tao ,
CHEN Junlin ,
QIAO Lin ,
WANG Li ,
CHENG Lixian ,
LI Aike ,
WANG Weiwei

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1. Institute of Grain Quality Nutrition, Academy of National Food and Strategic Reserves Administration, Beijing 100037, China;
2. College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China

Received date: 2021-07-30

Online published: 2022-02-15

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Abstract

This experiment was conducted to investigate the effects of double-embedded Lactobacillus plantarum and Enterococcus faecium on growth performance, immune and antioxidant function and intestinal microflora of broilers. One hundred and forty-four Arbor Acres (AA) male broilers of one-day-old with similar body weight were randomly divided into 4 groups with 6 replicates per group and 6 broilers per replicate. Broilers in the control group were fed a basal diet, and those in in the experimental groups were fed the basal diet supplemented with 1 g/kg embedded Lactobacillus plantarum (live bacterial count was 1.0×10¹⁰ CFU/g) (ELP group), 1 g/kg embedded Enterococcus faecium (live bacterial count was 1.0×10¹⁰ CFU/g) (EEF group) and 1 g/kg embedded Lactobacillus plantarum+1 g/kg embedded Enterococcus faecium (ELE group), respectively. The experiment lasted for 42 days. The results showed as follows:1) compared with the control group, the final body weight and average daily gain (ADG) of broilers from 1 to 21 days of age in the experimental groups were significantly increased (P<0.05), and the final body weight and ADG in ELP group were increased by 12.77% and 13.86% compared with the control group, respectively. 2) At 21 days of age, compared with the control group, the contents of immunoglobulin G and immunoglobulin A (IgA) in serum of broilers in ELE group were significantly increased (P<0.05), and the contents of IgA and immunoglobulin M in serum of broilers in EEF group were significantly increased (P<0.05). At 21 and 42 days of age, compared with the control group, the contents of tumor necrosis factor-α, interleukin-2 and interleukin-6 in serum of broilers in the experimental groups were significantly decreased (P<0.05), and the interleukin-10 content in serum was significantly increased (P<0.05), while the improvement effect in ELE group on cytokines was the strongest. 3) Compared with the control group, the activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) and total antioxidant capacity in serum of broilers at 21 days of age and in liver at 21 and 42 days of age in the experimental groups were significantly increased (P<0.05), the activities of GSH-Px and SOD in serum at 42 days of age were significantly increased (P<0.05), the malondialdehyde (MDA) content in liver at 21 and 42 days of age was significantly decreased (P<0.05), the MDA content in serum at 42 days of age in EEF group and ELE group was significantly decreased (P<0.05), and ELE group had the best antioxidant effect. 4) Compared with the control group, the fecal skatole content of broilers in ELP group was significantly decreased (P<0.05), and the fecal indole content had a decreasing trend (P>0.05). 5) Compared with the control group, the Chao1 index and ACE index in cecal microflora of broilers in ELP group were significantly increased (P<0.05). At phylum level, compared with the control group, there was no significant difference in the relative abundance of cecal microflora of broilers in the experimental groups (P>0.05), and the dominant microflora in all groups was Firmicutes, followed by Bacteroidetes. At genus level, compared with the control group, the unidentified Lachnospiraceae relative abundance in cecal microflora in the experimental groups was significantly decreased (P<0.05); the Faecalibacterium relative abundance in ELP group was significantly decreased (P<0.05), the f_Ruminococcaceae relative abundance was significantly increased (P<0.05), and the Lactobacillus relative abundance was increased by 20.11 times (P>0.05). In conclusion, dietary embedded Lactobacillus plantarum can significantly improve the growth performance and the intestinal microflora composition, and reduce the fecal odorous substance content of broilers from 1 to 21 days of age. The supplementation of embedded Lactobacillus plantarum and embedded Enterococcus faecium and their combination can significantly improve the immune and antioxidant function of broilers, and the combined supplementation has the better effect.

Key words： embedded Lactobacillus plantarum; embedded Enterococcus faecium; growth performance; immune and antioxidant function; intestinal microflora; broilers

Cite this article

SONG Dan , DUAN Tao , CHEN Junlin , QIAO Lin , WANG Li , CHENG Lixian , LI Aike , WANG Weiwei . Effects of Embedded Lactobacillus plantarum and Enterococcus faecium on Growth Performance, Immune and Antioxidant Function and Intestinal Microflora of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 877 -887 . DOI: 10.3969/j.issn.1006-267x.2022.02.021

References

[1] REVELL B J.One man's meat…2050?Ruminations on future meat demand in the context of global warming[J].Journal of Agricultural Economics, 2015, 66(3):573-614.

[2] WANG Y W, DONG Z L, SONG D, et al.Effects of microencapsulated probiotics and prebiotics on growth performance, antioxidative abilities, immune functions, and caecal microflora in broiler chickens[J].Food and Agricultural Immunology, 2018, 29(1):859-869.

[3] AYALA D I, COOK P W, FRANCO J G, et al.A systematic approach to identify and characterize the effectiveness and safety of novel probiotic strains to control foodborne pathogens[J].Frontiers in Microbiology, 2019, 10:1108.
[4] DONG Z L, WANG Y W, SONG D, et al.Effects of microencapsulated probiotics and plant extract on antioxidant ability, immune status and caecal microflora in Escherichia coli K88-challenged broiler chickens[J].Food and Agricultural Immunology, 2019, 30(1):1123-1134.

[5] HUANG L Q, LUO L P, ZHANG Y R, et al.Effects of the dietary probiotic, Enterococcus faecium NCIMB11181, on the intestinal barrier and system immune status in Escherichia coli O78-challenged broiler chickens[J].Probiotics and Antimicrobial Proteins, 2019, 11(3):946-956.

[6] YANG X, LIANG S S, GUO F S, et al.Gut microbiota mediates the protective role of Lactobacillus plantarum in ameliorating deoxynivalenol-induced apoptosis and intestinal inflammation of broiler chickens[J].Poultry Science, 2020, 99(5):2395-2406.

[7] WU Y P, WANG B K, ZENG Z H, et al.Effects of probiotics Lactobacillus plantarum 16 and Paenibacillus polymyxa 10 on intestinal barrier function, antioxidative capacity, apoptosis, immune response, and biochemical parameters in broilers[J].Poultry Science, 2019, 98(10):5028-5039.

[8] WANG S, PENG Q, JIA H M, et al.Prevention of Escherichia coli infection in broiler chickens with Lactobacillus plantarum B₁[J].Poultry Science, 2017, 96(8):2576-2586.

[9] DING S J, WANG Y W, YAN W X, et al.Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge[J].PLoS One, 2019, 14(6):e0212079.
[10] MI J D, CHEN X, LIAO X D.Screening of single or combined administration of 9 probiotics to reduce ammonia emissions from laying hens[J].Poultry Science, 2019, 98(9):3977-3988.

[11] WU Y Y, ZHEN W R, GENG Y Q, et al.Effects of dietary Enterococcus faecium NCIMB 11181 supplementation on growth performance and cellular and humoral immune responses in broiler chickens[J].Poultry Science, 2019, 98(1):150-163.

[12] WANG W W, CAI H Y, ZHANG A R, et al.Enterococcus faecium modulates the gut microbiota of broilers and enhances phosphorus absorption and utilization[J].Animals, 2020, 10(7):1232.
[13] ROSS R P, DESMOND C, FITZGERALD G F, et al.Overcoming the technological hurdles in the development of probiotic foods[J].Journal of Applied Microbiology, 2005, 98(6):1410-1417.

[14] CHÁVARRI M, MARAÑÓN I, VILLARÁN M C.Encapsulation technology to protect probiotic bacteria[M]//RIGOBELO E.Probiotics.London:IntechOpen, 2012.
[15] KNARREBORG A, BECK J, JENSEN M T, et al.Effect of non-starch polysaccharides on production and absorption of indolic compounds in entire male pigs[J].Animal Science, 2002, 74(3):445-453.

[16] ZHANG G J, LI B, GUO F, et al.Taxonomic relatedness and environmental pressure synergistically drive the primary succession of biofilm microbial communities in reclaimed wastewater distribution systems[J].Environment International, 2019, 124:25-37.
[17] TRABELSI I, KTARI N, BEN SLIMA S, et al.Effects of supplementation with L. plantarum TN8 encapsulated in alginate-chitosan in broiler chickens[J].International Journal of Biological Macromolecules, 2016, 89:677-681.
[18] WU S R, LIU Y L, DUAN Y L, et al.Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens[J].Journal of Animal Science and Biotechnology, 2018, 9:74.
[19] PENG Q, ZENG X F, ZHU J L, et al.Effects of dietary Lactobacillus plantarum B₁ on growth performance, intestinal microbiota, and short chain fatty acid profiles in broiler chickens[J].Poultry Science, 2016, 95(4):893-900.

[20] MOHAMMED A A, JIANG S, JACOBS J A, et al.Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, and immune response of broiler chickens reared under heat stress[J].Poultry Science, 2019, 98(10):4408-4415.

[21] HUMAM A M, LOH T C, FOO H L, et al.Effects of feeding different postbiotics produced by Lactobacillus plantarum on growth performance, carcass yield, intestinal morphology, gut microbiota composition, immune status, and growth gene expression in broilers under heat stress[J].Animals, 2019, 9(9):644.
[22] 马渭青, 王思博, 杨季, 等.饲粮中添加超氧化物歧化酶模拟物对肉鸡生长性能、血清免疫指标及肠道抗氧化指标和消化酶活性的影响[J].动物营养学报, 2020, 32(1):432-439. MA W Q, WANG S B, YANG J, et al.Effects of dietary superoxide dismutase mimics on growth performance, serum immune indexes and intestinal antioxidant indexes and digestive enzyme activities of broilers[J].Chinese Journal of Animal Nutrition, 2020, 32(1):432-439.(in Chinese)
[23] CAO L, WU X H, BAI Y L, et al.Anti-inflammatory and antioxidant activities of probiotic powder containing Lactobacillus plantarum 1.2567 in necrotic enteritis model of broiler chickens[J].Livestock Science, 2019, 223:157-163.
[24] SCHEER S, RUNTING J, BRAMHALL M, et al.The methyltransferase DOT1L controls activation and lineage integrity in CD4⁺ T cells during infection and inflammation[J].Cell Reports, 2020, 33(11):108505.
[25] MOLLAZADEH H, CICERO A F G, BLESSO C N, et al.Immune modulation by curcumin:the role of interleukin-10[J].Critical Reviews in Food Science and Nutrition, 2019, 59(1):89-101.

[26] ZHAO H P, ZHANG F K, CHAI J, et al.Effect of lactic acid bacteria on Listeria monocytogenes infection and innate immunity in rabbits[J].Czech Journal of Animal Science, 2020, 65(1):23-30.

[27] HU Y N, WANG Y W, LI A K, et al.Effects of fermented rapeseed meal on antioxidant functions, serum biochemical parameters and intestinal morphology in broilers[J].Food and Agricultural Immunology, 2016, 27(2):182-193.

[28] CAPCAROVA M, WEISS J, HRNCAR C, et al.Effect of Lactobacillus fermentum and Enterococcus faecium strains on internal milieu, antioxidant status and body weight of broiler chickens[J].Journal of Animal Physiology and Animal Nutrition, 2010, 94(5):e215-e224.
[29] YADAV S, JHA R.Strategies to modulate the intestinal microbiota and their effects on nutrient utilization, performance, and health of poultry[J].Journal of Animal Science and Biotechnology, 2019, 10(1):2.
[30] GUO J R, DONG X F, LIU S, et al.High-throughput sequencing reveals the effect of Bacillus subtilis CGMCC 1.921 on the cecal microbiota and gene expression in ileum mucosa of laying hens[J].Poultry Science, 2018, 97(7):2543-2556.

[31] 张沛.肉仔鸡粪臭素产生的基本规律及与肠道微生物组成的变化关系研究[D].硕士学位论文.沈阳:沈阳农业大学, 2016. ZHANG P.Research on the basic laws of skatole production and its variation with intestinal microbial components in broilers[D].Master's Thesis.Shenyang:Shenyang Agricultural University, 2016.(in Chinese)
[32] CHEN D F, JIN D C, HUANG S M, et al.Clostridium butyricum, a butyrate-producing probiotic, inhibits intestinal tumor development through modulating Wnt signaling and gut microbiota[J].Cancer Letters, 2020, 469:456-467.
[33] RINTTILÄ T.APAJALAHTI J.Intestinal microbiota and metabolites-implications for broiler chicken health and performance[J].Journal of Applied Poultry Research, 2013, 22(3):647-658.

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