Bacillus coagulans BC-HYI Improving Lipopolysaccharide Induced Intestinal Injury in Layer Chickens

HU Ting; ZHANG Hua; WU Qiong; ZHOU Bo; LU Tianhang; CUI Defeng; ZHANG Yonghong

doi:10.3969/j.issn.1006-267x.2022.02.027

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 2: 951 - 960

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.027

RESEARCH PAPER

Bacillus coagulans BC-HYI Improving Lipopolysaccharide Induced Intestinal Injury in Layer Chickens

HU Ting ,
ZHANG Hua ,
WU Qiong ,
ZHOU Bo ,
LU Tianhang ,
CUI Defeng ,
ZHANG Yonghong

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1. Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206, China;
2. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071000, China;
3. China Animal Husbandry Industry Co., Ltd., Beijing 100070, China

Received date: 2021-07-22

Online published: 2022-02-15

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Abstract

The aim of this study was to explore Bacillus coagulans BC-HYI improving lipopolysaccharide (LPS) induced intestinal injury in layer chickens. The 180 one-day-old Jinghong layer chickens were randomly divided into 5 groups with 6 replicates in each group and 6 layer chickens in each replicate. The weight between replicates was close. The blank control group (CON group) and the model group (LPS group) were fed a basic diet and continuously perfused with normal saline. The three probiotic groups were fed the basic diet and continuously perfused with 10⁶, 10⁷ and 10⁸ CFU/mL Bacillus coagulans BC-HYI, respectively for 28 days. After 28 days, LPS was perfused with a concentration of 2 mg/kg and a dose of 200 μL/chick, the test period was 6 h, and they were recorded as LOW+LPS, MID+LPS and HIGH+LPS groups, respectively. After 6 h, blood was collected, and the duodenum, jejunum, ileum and jejunal mucosa were collected. The results showed that compared with the CON group, after LPS administration, the ratio of villus length to crypt depth (V/C) in intestine of duodenum, jejunum and ileum and the mRNA relative expression levels of mucin 2 (MUC2), Occludin and zonula occludens protein-1 (ZO-1) in jejunal mucosa decreased significantly (P<0.05); and serum diamine oxidase (DAO) activity, D-lactic acid (D-Lac) and tumor necrosis factor-α (TNF-α) contents, jejunal mucosal secretory immunoglobulin A (SIgA) and interleukin-10 (IL-10) contents, the mRNA relative expression levels and protein expression levels of Toll like receptor 4 (TLR4) and nuclear factor-κB (NF-κB) in jejunal mucosal significantly increased (P<0.05). Compared with LPS group, medium and high doses of Bacillus coagulans BC-HYI significantly increased the V/C in intestine of duodenum, jejunum and ileum (P<0.01), and the mRNA relative expression levels and protein expression levels of TLR4 and NF-κB in jejunal mucosa significantly decreased (P<0.01), and the DAO activity and D-Lac content in serum significantly decreased (P<0.05); Bacillus coagulans BC-HYI significantly increased the relative mRNA expression levels of MUC2, Occludin and ZO-1 in jejunal mucosa (P<0.05); there was no significant difference in the contents of SIgA and IL-10 in jejunal mucosa (P>0.05). In conclusion, Bacillus coagulans BC-HYI can alleviate LPS injury, reduce intestinal permeability and down regulate the expression of gene and protein of TLR4 and NF-κB in TLR4/NF-κB signaling pathway, so as to improve the intestinal injury after LPS administration and improve the intestinal health of layer chickens.

Key words： Bacillus coagulans BC-HYI; layer chickens; LPS; intestinal mucosal permeability; TLR4 signaling pathway

Cite this article

HU Ting , ZHANG Hua , WU Qiong , ZHOU Bo , LU Tianhang , CUI Defeng , ZHANG Yonghong . Bacillus coagulans BC-HYI Improving Lipopolysaccharide Induced Intestinal Injury in Layer Chickens[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 951 -960 . DOI: 10.3969/j.issn.1006-267x.2022.02.027

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