RESEARCH PAPER

Effects of Small Peptide Supplementation in Diets with Fermented Mixed Meals on Growth Performance, Nutrient Apparent Digestibility and Serum Biochemical Indices of Beef Cattle

  • ZENG Yu ,
  • LIU Yao ,
  • ZHANG Kanglin ,
  • PENG Zhongli ,
  • XIAO Weiwei ,
  • YU Xingyu ,
  • WU Huazhuo
Expand
  • 1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Utilization, College of Animal & Veterinary Sciences, Southwest Minzu University, Chengdu 610041, China;
    2. Institute of Animal Husbandry of Ganzi Autonomous Prefecture of Sichuan Province, Kangding 626000, China;
    3. Chengdu Mytech Biotech Co., Ltd., Chengdu 610000, China

Received date: 2021-07-12

  Online published: 2022-02-15

Abstract

This study was to investigate the effects of small peptide supplementation in diets with fermented mixed meals on the growth performance, nutrient apparent digestibility and serum biochemical indices of beef cattle, explored the appropriate supplementation level of small peptides in diets with fermented mixed meals. Twenty-eight healthy Simmental crossbred cattle[(481.43±36.36) kg of initial weight] were randomly divided into 4 groups with 7 cattle in each group, and were fed total mixed ration diets with small peptide supplementation levels with 0 (group Ⅰ), 0.5% (group Ⅱ), 1.0% (group Ⅲ), and 1.5% (group Ⅳ), respectively. The pre-test period lasted for 25 days and the trial lasted for 60 days. The results showed as follows:1) with the increase of dietary small peptide levels, the average daily gain of beef cattle presented a tendency quadratic curve (P=0.062), and the feed/gain showed a quadratic curve (P<0.05), group Ⅲ had the highest average daily gain and the lowest feed/gain. 2) The apparent digestibility of organic matter had a quadratic curve with the increase of the levels of small peptide (P<0.05); and the apparent digestibility of crude protein of group Ⅲ was significantly higher than that of group Ⅳ (P<0.05), and it showed a tendency of quadratic curve (P=0.087). 3) With the increase of dietary small peptide levels, the activities of alanine aminotransferase, aspartate aminotransferase and the content of urea nitrogen were increased in a quadratic curve (P<0.05), and the contents of total cholesterol and glucose presented a tendency of difference (P=0.096, P=0.055). In conclusion, small peptide supplementation in diets with fermented mixed meals can improve liver function, increase nutrient apparent digestibility and growth performance of beef cattle. Under the conditions of this study, and the appropriate level of small peptides in diets with fermented mixed meals of beef cattle is 1.0%.

Cite this article

ZENG Yu , LIU Yao , ZHANG Kanglin , PENG Zhongli , XIAO Weiwei , YU Xingyu , WU Huazhuo . Effects of Small Peptide Supplementation in Diets with Fermented Mixed Meals on Growth Performance, Nutrient Apparent Digestibility and Serum Biochemical Indices of Beef Cattle[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1058 -1066 . DOI: 10.3969/j.issn.1006-267x.2022.02.037

References

[1] 许欣, 刘洋洋, 葛向阳, 等.蛋白质发酵饲料研究进展[J].饲料工业, 2020, 41(20):16-25. XU X, LIU Y Y, GE X Y, et al.Research progress of protein fermented feed[J].Feed Industry, 2020, 41(20):16-25.(in Chinese)
[2] 刘长松, 潘忠礼, 何荣海, 等.混菌固态发酵菜粕对肉仔鸡生长性能、血清生化指标及免疫功能的影响[J].中国家禽, 2015, 37(24):24-27. LIU C S, PAN Z L, HE R H, et al.Effects of mixed bacteria solid-state fermented rapeseed meal on growth performance, serum biochemical parameters and immune function of broilers[J].China Poultry, 2015, 37(24):24-27.(in Chinese)
[3] 黄洋, 杜涛, 朱春华.小肽制品对凡纳滨对虾生长性能及其非特异性免疫能力的影响[J].饲料研究, 2019, 42(12):20-23. HUANG Y, DU T, ZHU C H.Effects of small peptide products on growth performance and nonspecific immunity of Litopenaeus vannamei[J].Feed Research, 2019, 42(12):20-23.(in Chinese)
[4] 王文娟.瘤胃灌注小肽对肉牛瘤胃发酵与营养物质消化代谢的影响[D].硕士学位论文.泰安:山东农业大学, 2011. WANG W J.Effects of small peptide on ruminal fermentation and digestion of nutrition on beef cattle[D].Master's Thesis.Tai'an:Shandong Agricultural University, 2011.(in Chinese)
[5] VOLDEN H, MYDLAND L T, OLAISEN V.Apparent ruminal degradation and rumen escape of soluble nitrogen fractions in grass and grass silage administered intraruminally to lactating dairy cows[J].Journal of Animal Science, 2002, 80(10):2704-2716.
[6] 乔惠敏.大豆肽在动物生产中的作用及应用[J].四川畜牧兽医, 2018, 45(8):40-41. QIAO H M.The function and application of soybean peptide in animal production[J].Sichuan Animal & Veterinary Sciences, 2018, 45(8):40-41.(in Chinese)
[7] 张智安, 李世易, 武刚, 等.蜜蜂肽对育肥湖羊生长性能和瘤胃微生物区系的影响[J].动物营养学报, 2020, 32(2):756-764. ZHANG Z A, LI S Y, WU G, et al.Effects of honeybee peptide on growth performance and rumen microflora of fattening Hu sheep[J].Chinese Journal of Animal Nutrition, 2020, 32(2):756-764.(in Chinese)
[8] REN Z H, YUAN W, DENG H D, et al.Effects of antibacterial peptide on cellular immunity in weaned piglets[J].Journal of Animal Science, 2015, 93(1):127-134.  
[9] 刘双."富力肽"对泌乳中后期奶牛生产性能的影响研究[D].硕士学位论文.银川:宁夏大学, 2019. LIU S.Effect of "L&F" peptide on performance of dairy cows in the middle and late lactation period[D].Master's Thesis.Yinchuan:Ningxia University, 2019.(in Chinese)
[10] WANG W J, YANG W R, WANG Y, et al.Effects of soybean small peptides on rumen fermentation and on intestinal and total tract digestion of Luxi yellow cattle[J].Asian-Australasian Journal of Animal Sciences, 2013, 26(1):72-81.  
[11] 黎凌铄, 彭忠利, 陈仕勇, 等.小肽与酵母培养物对舍饲牦牛瘤胃微生物多样性和发酵参数的影响[J].中国饲料, 2021(5):16-23. LI L S, PENG Z L, CHEN S Y, et al.Effects of small peptide and yeast culture on rumen microbial diversity and fermentation parameters of yaks[J].China Feed, 2021(5):16-23.(in Chinese)
[12] 苗建军, 彭忠利, 高彦华, 等.日粮中添加小肽对育肥牦牛生产性能和消化道PepT1 mRNA表达的影响[J].中国农业科学, 2020, 53(23):4950-4960. MIAO J J, PENG Z L, GAO Y H, et al.Effects of dietary small peptides on production performance and expression of PepT1 mRNA in digestive tract of fattening yaks[J].Scientia Agricultura Sinica, 2020, 53(23):4950-4960.(in Chinese)
[13] 中华人民共和国农业部.肉牛饲养标准:NY/T 815-2004[S].北京:中国农业出版社, 2004. Ministry of Agriculture of the People's Republic of China.Feeding standard of beef cattle:NY/T 815-2004[S].Beijing:China Agriculture Press, 2004.(in Chinese)
[14] 张丽英.饲料分析及饲料质量检测技术[M].2版.北京:中国农业大学出版社, 2003. ZHANG L Y.Feed analysis and quality testing technology[M].2nd ed.Beijing:China Agricultural University Press, 2003.(in Chinese)
[15] VAN SOEST P J, ROBERTSON J B, LEWIS B A.Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science, 1991, 74(10):3583-3597.  
[16] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.饲料中盐酸不溶灰分的测定:GB/T 23742-2009[S].北京:中国标准出版社, 2009. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.Animal feeding stuffs-determination of ash insoluble in hydrochloric acid:GB/T 23742-2009[S]. Beijing:Standards Press of China, 2009(in Chinese)
[17] 司燕玲, 彭忠利, 高彦华, 等.小肽与酵母培养物对舍饲牦牛生产性能、养分表观消化率和血清生化指标的影响[J].中国饲料, 2020(3):56-61. SI Y L, PENG Z L, GAO Y H, et al.Effects of small peptides and yeast cultures on production performance, apparent digestibility of nutrients and blood biochemical parameters in yaks[J].China Feed, 2020(3):56-61.(in Chinese)
[18] 宋增廷.谷胱甘肽对肉羊瘤胃发酵、生长及肉质调控作用的研究[D].硕士学位论文.大庆:黑龙江八一农垦大学, 2008. SONG Z T.Studies on manipulation of rumen fermentation, growth and meat quality in fattening lambs by adding glutathione[D].Master's Thesis.Daqing:Heilongjiang Bayi Agricultural University, 2008.(in Chinese)
[19] 刘麟, 王书杰, 潘春晖, 等.小肽复合剂对育肥猪的生长性能及胴体和肉质性状的影响效应研究[J].饲料工业, 2019, 40(3):35-38. LIU L, WANG S J, PAN C H, et al.Effects of small peptide complexes on growth performance, carcass and meat quality traits of growing-finishing pigs[J].Feed Industry, 2019, 40(3):35-38.(in Chinese)
[20] 涂瑞, 苗建军, 彭忠利, 等.不同精粗比日粮中添加小肽对牦牛瘤胃体外发酵特性的影响[J].草业学报, 2020, 29(3):78-88. TU R, MIAO J J, PENG Z L, et al.An in vitro study of dietary concentrate:forage ratio and small peptide supplementation effects on ruminal fermentation parameters of yaks[J].Acta Prataculturae Sinica, 2020, 29(3):78-88.(in Chinese)
[21] EVANS N J, BROWN J M, MURRAY R D, et al.Characterization of novel bovine gastrointestinal tract Treponema isolates and comparison with bovine digital dermatitis treponemes[J].Applied and Environmental Microbiology, 2011, 77(1):138-147.  
[22] 李岚捷, 成述儒, 刁其玉, 等.不同NFC/NDF水平饲粮对犊牛瘤胃发酵参数和微生物区系多样性的影响[J].畜牧兽医学报, 2017, 48(12):2347-2357. LI L J, CHENG S R, DIAO Q Y, et al.Effects of diets with different NFC/NDF levels on the rumen fermentation parameters and bacterial community in male calves[J].Acta Veterinaria et Zootechnica Sinica, 2017, 48(12):2347-2357.(in Chinese)
[23] HOOVER W H.Chemical factors involved in ruminal fiber digestion[J].Journal of Dairy Science, 1986, 69(10):2755-2766.  
[24] JONES D F, HOOVER W H, MILLER WEBSTER T K.Effects of concentrations of peptides on microbial metabolism in continuous culture[J].Journal of Animal Science, 1998, 76(2):611-616.  
[25] 李琍, 丁角立.肽对体外混合培养瘤胃微生物发酵和生长影响的研究[J].畜牧兽医学报, 2000, 31(2):113-119. LI L, DING J L.Effects of peptides on the fermentation and growth of mixed ruminal microorganisms in vitro[J].Acta Veterinaria et Zootechnica Sinica, 2000, 31(2):113-119.(in Chinese)
[26] REYNAL S M, IPHARRAGUERRE I R, LIÑEIRO M, et al.Omasal flow of soluble proteins, peptides, and free amino acids in dairy cows fed diets supplemented with proteins of varying ruminal degradabilities[J].Journal of Dairy Science, 2007, 90(4):1887-1903.  
[27] 康怀艳.益生菌发酵饲料对奶山羊血清生化指标和产奶性能的影响[D].硕士学位论文.杨凌:西北农林科技大学, 2019. KANG H Y.Effect of probiotic fermented feed on serum biochemical parameters and milk performance of dairy goats[D].Master's Thesis.Yangling:Northwest A&F University, 2019.(in Chinese)
[28] 孙海元.小肽和半胱胺对生长肥育猪生产性能的影响及机理研究[D].硕士学位论文.邯郸:河北工程大学, 2013. SUN H Y.Effect of peptide and cysteamine on growth performance and mechanism in growing pigs[D].Master's Thesis.Handan:Hebei University of Engineering, 2013.(in Chinese)
[29] 王建红, 刁其玉, 许先查, 等.日粮Lys、Met和Thr添加模式对0-2月龄犊牛生长性能、消化代谢与血清学生化指标的影响[J].中国农业科学, 2011, 44(9):1898-1907. WANG J H, DIAO Q Y, XU X C, et al.Effects of dietary addition pattern of lysine, methionine and threonine in the diet on growth performance, nutrient digestion and metabolism, and serum biochemical parameters in calves at the ages of 0 to 2 months[J].Scientia Agricultura Sinica, 2011, 44(9):1898-1907.(in Chinese)
[30] 何平平, 欧阳新平, 唐艳艳, 等.甘油三酯水平升高与动脉粥样硬化性心血管疾病的关系的研究新进展[J].中国动脉硬化杂志, 2013, 21(10):951-954. HE P P, OU Y X P, TANG Y Y, et al.Research advances of relationship between triglyceride level and atherosclerotic cardiovascular disease[J].Chinese Journal of Arteriosclerosis, 2013, 21(10):951-954.(in Chinese)
[31] 苏玲玲, 托乎提·阿及德, 张国庭, 等.全株玉米青贮比例对疆岳驴生长性能、营养物质表观消化率及血清生化指标的影响[J].动物营养学报, 2021, 33(4):2083-2092. SU L L, TUOHUTI. A, ZHANG G T, et al.Effects of whole corn silage proportion on growth performance nutrient apparent digestibility and serum biochemical indices of Jianyue donkeys[J].Chinese Journal of Animal Nutrition, 2021, 33(4):2083-2092.(in Chinese)
[32] 张振宇, 梁春年, 姚喜喜, 等.日粮不同营养水平对牦牛生产性能、屠宰指标和血清生化指标的影响[J].畜牧兽医学报, 2021, 52(1):135-143. ZHANG Z Y, LIANG C N, YAO X X, et al.Effects of different nutrition levels of diets on production performance, slaughter indexes and serum biochemical indexes of yak[J].Acta Veterinaria et Zootechnica Sinica, 2021, 52(1):135-143.(in Chinese)
[33] SATTER L D, SLYTER L L.Effect of ammonia concentration of rumen microbial protein production in vitro[J].The British Journal of Nutrition, 1974, 32(2):199-208.  
[34] YANG W Z, BEAUCHEMIN K A, RODE L M.Effects of grain processing, forage to concentrate ratio, and forage particle size on rumen pH and digestion by dairy cows[J].Journal of Dairy Science, 2001, 84(10):2203-2216.  
Outlines

/