RESEARCH PAPER

Effects of Nano-Zinc Oxide on Performance, Serum Indexes and Expression of Intramuscular Fat Deposition Related Genes in Longissimus dorsi Muscle of Fattening Cattle

  • ZHONG Qiqi ,
  • LI Lizhi ,
  • XING Qingfeng ,
  • GUO Dongsheng ,
  • ZHANG Haibo ,
  • GUAN Weikun
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  • 1. Yichun Key Laboratory of Functional Agriculture and Ecological Environment, Engineering Technology Research Center of Jiangxi Universities and Colleges for Selenium Agriculture, College of Life Science and Resources and Environment, Yichun University, Yichun 336000, China;
    2. College of Animal Science & Technology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan 430000, China

Received date: 2021-07-18

  Online published: 2022-02-15

Abstract

The purpose of this experiment was to study the effects of nano-zinc oxide on performance, serum indexes and intramuscular fat (IMF) deposition related enzyme activities and gene expression in longissimus dorsi muscle of fattening cattle. Sixteen western hybrid steers (Simmental×local yellow cattle) were randomly divided into 2 groups with 8 replicates per group and 1 cattle per replicate. In control group, cattle were fed a basal diet (without zinc), and those in experimental nano-zinc oxide group were fed the basal diet supplemented with 80 mg/kg zinc (nano-zinc oxide form). The experiment lasted for 60 days. The results showed as follows:1) compared with the control group, adding nano-zinc oxide significantly increased the average daily gain (P<0.05), and significantly decreased the feed/gain (P<0.05). 2) Compared with the control group, adding nano-zinc oxide extremely significantly increased the contents of serum free fatty acid, triglyceride, total cholesterol, high density lipoprotein, low density lipoprotein, glucose and insulin like growth factor-1 (P<0.01), significantly increased insulin content (P<0.05), and extremely significantly decreased the ratio of glucose to insulin (P<0.01). 3) Compared with the control group, adding nano-zinc oxide significantly increased the IMF content and acetyl coenzyme A carboxylase (ACC) activity in longissimus dorsi muscle (P<0.05), extremely significantly decreased the hormone sensitive lipase (HSL) activity (P<0.01), and significantly decreased the activities of carnitine transferase-1 (CPT-1) and lipoprotein lipase (LPL) (P<0.05). 4) Compared with the control group, adding nano-zinc oxide significantly increased the expression levels of sterol regulatory element binding protein-1 (SREBP-1), peroxidase proliferation-activated receptor γ (PPARγ), fatty acid synthetase (FAS) and ACC genes in longissimus dorsi muscle (P<0.05), and significantly decreased the expression levels of HSL and CPT-1 genes in longissimus dorsi muscle (P<0.05). In conclusion, dietary nano-zinc oxide (80 mg/kg zinc) can up-regulate the expression of fatty acid synthetase related genes (FAS, ACC, SREBP-1 and PPARγ), down-regulate the expression of fatty acid decompose-related genes (HSL and CPT-1), and enhance the activities of fat synthetases (FAS and ACC) in longissimus dorsi muscle of fattening cattle, inhibit the activities of lipolytic enzyme (HSL and CPT-1), promote the deposition of IMF, improve the muscle quality and performance of fattening cattle.

Cite this article

ZHONG Qiqi , LI Lizhi , XING Qingfeng , GUO Dongsheng , ZHANG Haibo , GUAN Weikun . Effects of Nano-Zinc Oxide on Performance, Serum Indexes and Expression of Intramuscular Fat Deposition Related Genes in Longissimus dorsi Muscle of Fattening Cattle[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1067 -1075 . DOI: 10.3969/j.issn.1006-267x.2022.02.038

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