Effects of Herbal Tea Residue on Ileum Morphology, Barrier Function and Flora Structure of Fattening Cattle

LI Ling; SUN Xiaohong; LIAN Xu; LUO Junyi; CHEN Ting; XI Qianyun; ZHANG Yongliang; SUN Jiajie

doi:10.3969/j.issn.1006-267x.2022.02.040

Chinese Journal of Animal Nutrition >

2022 , Vol. 34 >Issue 2: 1087 - 1097

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.040

RESEARCH PAPER

Effects of Herbal Tea Residue on Ileum Morphology, Barrier Function and Flora Structure of Fattening Cattle

LI Ling ,
SUN Xiaohong ,
LIAN Xu ,
LUO Junyi ,
CHEN Ting ,
XI Qianyun ,
ZHANG Yongliang ,
SUN Jiajie

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Key Laboratory of Animal Nutrition Control in Guangdong, College of Animal Science, South China Agricultural University, Guangzhou 510642, China

Received date: 2021-07-14

Online published: 2022-02-15

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Abstract

This experiment was conducted to study the effects of herbal tea residue on ileum morphology, barrier function and flora structure of fattening cattle. A total of 120 healthy Simmental crossbred beef cattle with similar body weight were randomly divided into two groups:control group (fed a basal diet) and experimental group (50% herbal tea residue replaced part of elephant grass), with 3 replicates in each group and 20 heads in each replicate. The experimental period lasted for 67 days, including 7 days of pre feeding period and 60 days of formal experimental period. At the end of the experimental period, the blood, jejunal tissue and jejunal contents were collected, and the villus height and crypt depth of jejunum were measured by hematoxylin-eosin (HE) staining; the concentrations of inflammatory factors, D-lactic acid (D-Lac) and diamine oxidase (DAO) in serum were measured by enzyme-linked immunosorbent assay (ELISA) kit; the mRNA expression levels of tight junction proteins of zonula occludens-1 (ZO-1), Occludin and Claudin-1 in jejunum were determined by fluorescence quantitative PCR; the 16S rDNA high-throughput sequencing was used to analyze the structure of jejunal flora. The results showed as follows:1) compared with the control group, the jejunal villi height of the experimental group was significantly increased (P<0.05), the serum D-Lac concentration was significantly decreased (P<0.05), and the mRNA expression level of tight junction protein ZO-1 in jejunum was significantly up-regulated (P<0.05). 2) At the phylum level, the Firmicutes relative abundances in the control group and the experimental group were 85.79% and 76.96%, respectively, and the Bacteroidetes relative abundances were 0.54% and 3.11%, respectively. At the genus level, the relative abundances of Romboutsia, Rothia and Blautia showed significant differences between the control group and the experimental group (P<0.05). 3) Pearson correlation analysis displayed that the jejunal villi height showed significant positive correlations with the relative abundances of Blautia, Brevundimonas, Rothia and Tetrasphaera (P<0.05), and the correlation coefficients ranged from 0.46 to 0.57; the serum D-Lac concentration showed significant positive correlations with the relative abundances of Romboutsia and Terrisporobacter (P<0.05), and the correlation coefficients were 0.56 and 0.62, respectively; the mRNA expression level of tight junction protein ZO-1 in jejunum showed a significant negative correlation with the relative abundances of Solobacterium (P<0.05), and correlation coefficient was -0.50. In conclusion, adding 50% herbal tea residue as functional roughage in the diet has positive effects on the jejunal villus height and barrier function of fattening cattle, the herbal tea residue may affect intestinal tissue morphology and barrier function by changing the composition and relative abundance of jejunal flora, and ultimately promote the intestinal health.

Key words： herbal tea residue; fattening cattle; jejunum; tissue morphology; barrier function; jejunal flora

Cite this article

LI Ling , SUN Xiaohong , LIAN Xu , LUO Junyi , CHEN Ting , XI Qianyun , ZHANG Yongliang , SUN Jiajie . Effects of Herbal Tea Residue on Ileum Morphology, Barrier Function and Flora Structure of Fattening Cattle[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1087 -1097 . DOI: 10.3969/j.issn.1006-267x.2022.02.040

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