RESEARCH PAPER

Effects of Anemoside B4 on Growth Performance and Serum Indices of Sucking Calves

  • LU Meng ,
  • HU Fengming ,
  • TU Yan ,
  • DIAO Qiyu
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  • Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs, Feed Research Institute of Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2021-07-30

  Online published: 2022-02-15

Abstract

The objective of this experiment was to investigate the effects of anemoside B4 on growth performance and serum indices of sucking calves. In this experiment, sixty newborn Holstein male calves were randomly divided into 4 groups (15 calves in each group) and fed diets supplemented with 0 (group C), 15 (group A1), 30 (group A2) and 45 (group A3) mg/d anemoside B4 in the milk replacer, respectively. The experiment lasted for 56 days, including 14 days of pre-experiment and 42 days of formal experiment. The body weight and size were taken every 2 weeks on an empty stomach, the feed intake and fecal score were recorded every day, and the serum hormone levels, antioxidant and immune indices of calves at 28 and 56 days of age were measured. The results showed as follows:1) dietary anemoside B4 had no significant effects on the body weight and average daily feed intake of sucking calves (P>0.05), the average daily gain of calves in group A3 was significantly higher than that in group C from 14 to 28 days of age (P<0.05), and the feed to gain ratio of calves in groups A2 and A3 was significantly lower than that in group C from 14 to 28 days of age (P<0.05). 2) Dietary anemoside B4 had no significant effects on the wither height, body steep length, hip width and heart girth of sucking calves (P>0.05). 3) Compared with group C, the fecal score and diarrhea rate of calves in groups A1, A2 and A3 were significantly decreased from 7 to 42 days of age (P<0.05). 4) At 28 days of age, the contents of growth hormone (GH) and interleukin-10 in serum and the activities of superoxide dismutase (SOD) and catalase (CAT) in serum of calves in groups A1, A2 and A3 were significantly higher than those in group C (P<0.05), and the contents of malondialdehyde (MDA), interleukin-8 and tumor necrosis factor-α in serum were significantly lower than those in group C (P<0.05); the glutathione peroxidase activity and contents of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) in serum of calves in groups A2 and A3 were significantly higher than those in group C (P<0.05), and the interleukin-6 (IL-6) content in serum was significantly lower than that in group C (P<0.05); the insulin-like growth factor Ⅰ (IGF-Ⅰ) content in serum of calves in group A3 was significantly higher than that in groups C, A1 and A2 (P<0.05), and the contents of IgG and IgM in serum were significantly higher than that in group A1 (P<0.05). At 56 days of age, there were no significant differences in serum GH, IgG and IgA contents among all groups (P>0.05); the IGF-Ⅰ content and SOD activity in serum of calves in group A3 tended to be significantly higher than those in group C (0.05<P<0.10), and the serum MDA content tended to be significantly lower than that in group C (0.05<P<0.10); the serum CAT activity in group A3was significantly higher than that in group C (P<0.05); the serum IgM content in groups C and A3 was significantly higher than that in group A1 (P<0.05); the serum IL-6 content in groups A1 and A2 tended to be significantly higher than that in group A3 (0.05<P<0.10), but had no significant difference compared with group C (P>0.05); the serum IGF-Ⅰ content in group A3 tended to be significantly higher than that in group C (0.05<P<0.01). In conclusion, the supplementation of anemoside B4 in the milk replacer can effectively promote the growth and development of the body, enhance the antioxidant and immune capacity of calves, and reduce the diarrhea rate, and the effect is better when the supplemental level is 45 mg/d.

Cite this article

LU Meng , HU Fengming , TU Yan , DIAO Qiyu . Effects of Anemoside B4 on Growth Performance and Serum Indices of Sucking Calves[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1098 -1108 . DOI: 10.3969/j.issn.1006-267x.2022.02.041

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