RESEARCH PAPER

Comparative Analysis of Cellulase Activity and Volatile Fatty Acid Contents in Cecum and Colon Chyme Liquid of Dezhou Donkey

  • ZHANG Zhenwei ,
  • ZHOU Qian ,
  • ZHENG Rui ,
  • WANG Yonghui ,
  • ZHU Mingxia ,
  • WANG Changfa
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  • Liaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252000, China

Received date: 2021-08-12

  Online published: 2022-02-15

Abstract

In order to determine the cellulase activities and the contents of volatile fatty acids (VFA) in the hindgut of donkeys, chyme liquid samples from cecum, ventral colon and dorsal colon of 9 Dezhou donkeys were collected and filtered by 4-layer gauze. The activities of carboxymethyl cellulase (CMCase), microcrystalline cellulase (AVI), xylan cellulase (Xylase), ferulic esterase (FAE) and acetylesterase (AE) were measured by spectrophotometer for three times. The results showed that the activities of CMCase, AVI and Xlyase increased gradually in cecum, ventral colon and dorsal colon chyme liquid (P<0.05), but there was no significant difference for the activity of FAE between the cecum and ventral colon chyme liquid. The AE activity in dorsal colon chyme liquid was significantly higher than that in cecum and ventral colon (P>0.05). In addition, the VFA content was determined through gas chromatograph. The molar ration of acetate was increased gradually in cecum, ventral colon and dorsal colon chyme liquid (P<0.05), while the molar ration of propionic acids was gradually decreased (P<0.05). In summary, it is confirmed that donkey cecum and colon chyme liquid has higher FAE and AE activities for catalytic hydrolysis of cell wall hemicellulose and intermolecular ester bond of lignin; in addition, the molar ration of acetate is gradually increased while the molar ration of propionate is decreased in donkey cecum, ventral colon and dorsal colon chyme liquid.

Cite this article

ZHANG Zhenwei , ZHOU Qian , ZHENG Rui , WANG Yonghui , ZHU Mingxia , WANG Changfa . Comparative Analysis of Cellulase Activity and Volatile Fatty Acid Contents in Cecum and Colon Chyme Liquid of Dezhou Donkey[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1151 -1157 . DOI: 10.3969/j.issn.1006-267x.2022.02.046

References

[1] ZHANG Z W, ZHAN Y D, HAN Y, et al.Estimation of liveweight from body measurements through best fitted regression model in Dezhou donkey breed[J].Journal of Equine Veterinary Science, 2021, 101:103457.
[2] HARRIS P A, ELLIS A D, FRADINHO M J, et al.Review:feeding conserved forage to horses:recent advances and recommendations[J].Animal, 2017, 11(6):958-967.  
[3] GLINSKY M J, SMITH R M, SPIRES H R, et al.Measurement of volatile fatty acid production rates in the cecum of the pony[J].Journal of Animal Science, 1976, 42(6):1465-1470.  
[4] EDWARDS J E, SHETTY S A, VAN DEN BERG P, et al.Multi-kingdom characterization of the core equine fecal microbiota based on multiple equine (sub) species[J].Animal Microbiome, 2020, 2(1):6.
[5] YUE Q, YANG H J, CAO Y C, et al.Feruloyl and acetyl esterase production of an anaerobic rumen fungus Neocallimastix sp. YQ2 effected by glucose and soluble nitrogen supplementations and its potential in the hydrolysis of fibrous feedstuffs[J].Animal Feed Science and Technology, 2009, 153(3/4):263-277.
[6] 苏少锋.蒙古马胃肠道细菌群落组成及纤维素分解菌的研究[D].博士学位论文.呼和浩特:内蒙古农业大学, 2019. SU S F.Characterization of gastrointestinal bacterial community and cellulose-decomposing bacteria in Mongolian horses[D].Ph.D.Thesis.Hohhot:Inner Mongolia Agricultural University, 2019.(in Chinese)
[7] 白洪志.降解纤维素菌种筛选及纤维素降解研究[D].博士学位论文.哈尔滨:哈尔滨工业大学, 2008. BAI H Z.Screening of cellulose degradation fungi and the study of its degradation characteristics[D].Ph.D.Thesis.Harbin:Harbin Institute of Technology, 2008.(in Chinese)
[8] 荆常亮, 李福昌, 王雪鹏, 等.日粮ADF水平对断奶至3月龄獭兔生长性能、氮代谢、纤维素酶活和盲肠发酵的影响[J].畜牧兽医学报, 2013, 44(9):1417-1424. JING C L, LI F C, WANG X P, et al.Effects of dietary ADF levels on growth performance, nitrogen metabolism, cellulase activity and caecum fermentation of Rex rabbits from weaning to 3-month-old[J].Acta Veterinaria et Zootechnica Sinica, 2013, 44(9):1417-1424.(in Chinese)
[9] 刘利林.日粮粗饲料水平对新疆驴饲料养分消化代谢与盲肠发酵特性的影响[D].博士学位论文.北京:中国农业大学, 2020. LIU L L.The effect of dietary forage level on nutrient digestion and metabolism and caecal fermentation characteristics in Xinjiang donkeys[D].Ph.D.Thesis.Beijing:China Agricultural University, 2020.(in Chinese)
[10] BARTOLOME B, FAULDS C B, TUOHY M, et al.Influence of different xylanases on the activity of ferulic acid esterase on wheat bran[J].Biotechnology and Applied Biochemistry, 1995, 22(1):65-73.
[11] WILLIAMSON G, KROON P A, FAULDS C B.Hairy plant polysaccharides:a close shave with microbial esterases[J].Microbiology, 1998, 144(Pt 8):2011-2023.
[12] 梁婷玉.驴胃肠道组织形态、酶活及微生物区系研究[D].硕士学位论文.兰州:甘肃农业大学, 2019. LIANG T Y.Study on gastrointestinal tract mucosal morphology, enzyme activity and microflora of donkeys[D].Master's Thesis.Lanzhou:Gansu Agricultural University, 2019.(in Chinese)
[13] 任清长.孕酮在瘤胃的转化及其对微生物消化代谢的调节作用[D].博士学位论文.北京:中国农业大学, 2014. REN Q C.Microbial transformation of progesterone in the rumen and its regulation in microbial digestion and metabolism[D].Ph.D.Thesis.Beijing:China Agricultural University, 2014.(in Chinese)
[14] PEARSON R A, ARCHIBALD R F, MUIRHEAD R H.The effect of forage quality and level of feeding on digestibility and gastrointestinal transit time of oat straw and alfalfa given to ponies and donkeys[J].The British Journal of Nutrition, 2001, 85(5):599-606.  
[15] MIYAJI M, UEDA K, HATA H, et al.Effect of grass hay intake on fiber digestion and digesta retention time in the hindgut of horses[J].Journal of Animal Science, 2014, 92(4):1574-1581.  
[16] 陈洪洋, 蔡俊, 林建国, 等.木聚糖酶的研究进展[J].中国酿造, 2016, 35(11):1-6. CHEN H Y, CAI J, LIN J G, et al.Research progress of xylanase[J].China Brewing, 2016, 35(11):1-6.(in Chinese)
[17] 黎大洪.不同日粮饲喂条件下荷斯坦肉牛瘤胃微生物发酵阿魏酸酯酶昼夜变化规律研究[D]. 硕士学位论文.北京:中国农业大学, 2009. LI D H.Diurnal variations of ferulic acid esterase activities in rumen fermentation effected by rations intaked in Holstein cattle[D].Master's Thesis.Beijing:China Agricultural University, 2014.(in Chinese)
[18] ARGENZIO R A, SOUTHWORTH M, STEVENS C E.Sites of organic acid production and absorption in the equine gastrointestinal tract[J].The American Journal of Physiology, 1974, 226(5):1043-1050.  
[19] VAN GYLSWYK N O.The effect of supplementing a low-protein hay on the cellulolytic bacteria in the rumen of sheep and on the digestibility of cellulose and hemicellulose[J].The Journal of Agricultural Science, 1970, 74(1):169-180.  
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