Differentially Expressed Genes and Signaling Pathways of Regulation of Dietary Lysine Level on Feed Intake of Guizhou Yellow Chickens Based on Transcriptome Sequencing Technology

WANG Xiaoya; RAO Yongchao; CHEN Dahai; TANG Hong; LIN Jiadong; ZHANG Fuping; SHI Xiaoli

doi:10.3969/j.issn.1006-267x.2022.02.056

2022 , Vol. 34 >Issue 2: 1247 - 1259

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.056

RESEARCH PAPER

Differentially Expressed Genes and Signaling Pathways of Regulation of Dietary Lysine Level on Feed Intake of Guizhou Yellow Chickens Based on Transcriptome Sequencing Technology

WANG Xiaoya ,
RAO Yongchao ,
CHEN Dahai ,
TANG Hong ,
LIN Jiadong ,
ZHANG Fuping ,
SHI Xiaoli

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1. Key Laboratory of Animal Genetics, Breeding and Reproduction in Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;
2. Guizhou Provincial Key Laboratory of Animal Genetics, Breeding and Reproduction, Guiyang 550025, China;
3. College of Animal Science, Guizhou University, Guiyang 550025, China;
4. Research Chicken Farm of Guizhou University, Guiyang 550025, China

Received date: 2021-08-22

Online published: 2022-02-15

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Abstract

This experiment aims to study the molecular mechanism of dietary lysine (Lys) level regulated feed intake of Guizhou yellow chickens by transcriptome sequencing (RNA-Seq) technique, and to explore the candidate genes and signaling pathways related to feed intake, to further increase the intake of poultry and to promote the efficiency of farming. A total of 400 one-day-old Guizhou yellow chickens were randomly divided into 4 groups with 5 replicates per group and 20 chickens per replicate. The dietary Lys levels of each group were 0.7%, 0.9%, 1.1% and 1.3%, respectively. The experiment lasted for 6 weeks. At 6 weeks of age, three Guizhou yellow chickens in each of the 0.9% Lys level group (b group, n=3) and 1.3% Lys level group (d group, n=3) with significant differences (P<0.01) in average daily feed intake were selected, collected the hypothalamus tissue and used for RNA-Seq analyze, GO functional enrichment and KEGG pathways of differential expression genes, ultimately, the RNA-Seq results were verified by real-time quantitative PCR (qRT-PCR). The results showed as follows:1) the average daily feed intake during 1 to 6 weeks of age of 0.7%, 0.9% and 1.1% Lys level groups was significantly higher than that of 1.3% Lys level group (P<0.01), and the 0.9% Lys level group was the highest. 2) The comparison rate of the sequencing reads produced by each sample to the chicken reference genome was greater than 89%, Q20 and Q30 contents were 97% and 93% respectively; a total of 200 6 differentially expressed genes were screened in b group and d group, which 1 275 genes were up-regulated, while 731 genes were down-regulated. The GO function was highly enriched in biological processes such as neuron projection morphogenesis, positive regulation of synaptic transmission and negative regulation of neurogenes; the KEGG pathway was significantly enriched to 19 pathways, among these pathways, the enriched phosphatidylinositol signaling system, forkhead box O signaling pathway, mammalian target of rapamycin signaling pathway, mitogen-activated protein kinase signaling pathway, neuroactive ligand-receptor interaction signaling pathway and adipocytokine signaling pathway, and these pathways could affect the feed intake of Guizhou yellow chickens. The screening of neuropeptide Y (NPY), proopiomelanocortin (POMC), agouti-related protein (AgRP), cocaine and amphetamine-regulated prepropeptide (CARTPT) enriched in the neuroactive ligand-receptor interaction and adipocytokine signaling pathway. The qRT-PCR results showed that the expression trends of 6 differentially expressed genes randomly selected were consistent with the RNA-Seq results. In summary, the average daily feed intake of 1 to 6 weeks of age of 0.7%, 0.9% and 1.1% Lys level group was significantly higher than that of 1.3% Lys level group. Different Lys levels may regulate the expression of NPY, AgRP, CARTPT, POMC and other related genes in hypothalamic tissue, acting on the neural active ligand-receptor interaction pathway and adipocytokine signaling pathway, thereby regulate the feed intake of Guizhou yellow chickens.

Key words： RNA-Seq; Guizhou yellow chickens; lysine; hypothalamus; feed intake

Cite this article

WANG Xiaoya , RAO Yongchao , CHEN Dahai , TANG Hong , LIN Jiadong , ZHANG Fuping , SHI Xiaoli . Differentially Expressed Genes and Signaling Pathways of Regulation of Dietary Lysine Level on Feed Intake of Guizhou Yellow Chickens Based on Transcriptome Sequencing Technology[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1247 -1259 . DOI: 10.3969/j.issn.1006-267x.2022.02.056

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