RESEARCH PAPER

Effects of Chicken Angiopoietin-Like Protein 4 Recombinant Protein on Fat Metabolism in Liver and Breast Muscle of Broilers

  • HOU Yanru ,
  • ZHAO Xu ,
  • HUANG Huashan ,
  • ZHANG Jinhong ,
  • JING Changwei
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  • 1. College of Agriculture and Forestry Science, Linyi University, Linyi 276000, China;
    2. Shandong Longda Bio-Products Co., Ltd., Linyi 276400, China;
    3. Shandong Yatai Zhonghui Group Co., Ltd., Weifang 262400, China

Received date: 2021-07-30

  Online published: 2022-02-15

Abstract

This experiment aimed to explore the effects of chicken angiopoietin-like protein 4 (ANGPTL4) recombinant protein on fat metabolism in liver and breast muscle of broilers. It was divided into 2 parts:animal experiment in vivo and cell experiment in vitro. In the animal experiment in vivo, thirty-six healthy 35-day-old Arbor Acres broilers under fasting condition were randomly divided into 6 groups with 6 replicates per group and 1 broiler per replicate. Broilers in the control group were intravenously injected with sterile normal saline, and others in experimental groups were intravenously injected 20, 100, 500, 2 500 and 12 500 ng/kg BW chicken ANGPTL4 recombinant protein, respectively. The injection dose was 550 μL, and the experiment period was 30 minutes. Three groups were established for the cell experiment in vitro, including the normal saline group, histidine-small ubiquitin-like modifier (His-SUMO) tag group and chicken ANGPTL4 recombinant protein group, which supplemented 5 μL sterile normal saline, His-SUMO tag protein (its content is consistent with that of chicken ANGPTL4 recombinant protein group) and chicken ANGPTL4 recombinant protein (250 pg/mL), respectively, with 5% CO2 and 37℃ incubated 24 h. The results showed as follows:1) compared with the control group, the liver fatty acid synthase (FAS) mRNA expression level of 20, 100, 500 and 2 500 ng/kg BW chicken ANGPTL4 recombinant protein groups and liver FAS activity of 500 ng/kg BW chicken ANGPTL4 recombinant protein group were significantly increased (P<0.05). With the chicken ANGPTL4 recombinant protein injection dose increased, the liver FAS mRNA expression level and activity showed linear and quadratic increase effects (P<0.05). 2) Compared with the control group, the liver malic enzyme (ME) mRNA expression level of 100, 500 and 2 500 ng/kg BW chicken ANGPTL4 recombinant protein groups and liver acetyl-CoA carboxylase (ACC) mRNA expression level of 12 500 ng/kg BW chicken ANGPTL4 recombinant protein group were significantly decreased (P<0.05). With the chicken ANGPTL4 recombinant protein injection dose increased, the liver ACC mRNA expression level and activity showed linear and quadratic decrease effects (P<0.05). Different injection dose of chicken ANGPTL4 recombinant protein had no significant effects on activities of ME and ACC in liver of broilers (P>0.05). 3) Compared with the control group, the breast muscle lipoprotein lipase (LPL) activity of 500 ng/kg BW chicken ANGPTL4 recombinant protein group was significantly increased (P<0.05). 4) The myoblasts triglyceride (TG) content of chicken ANGPTL4 recombinant protein group was significantly higher than that of normal saline group and His-SUMO tag group (P<0.05). In summary, chicken ANGPTL4 recombinant protein can regulate the mRNA expression and activity of fat synthesis related genes and enzyme in liver of broilers and promote fat deposition in breast muscle of broilers, and the 500 ng/kg BW injection dose has the better effect.

Cite this article

HOU Yanru , ZHAO Xu , HUANG Huashan , ZHANG Jinhong , JING Changwei . Effects of Chicken Angiopoietin-Like Protein 4 Recombinant Protein on Fat Metabolism in Liver and Breast Muscle of Broilers[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1260 -1267 . DOI: 10.3969/j.issn.1006-267x.2022.02.057

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