Repair Effects of Sodium Butyrate on Inflammatory Injury of Lipopolysaccharide-Induced Bovine Mammary Epithelial Cell Line

LI Lin; GONG Binbin; XU Changfeng; CAO Meng; LI Jianyuan; ZHAO Mei; TANG Weibin

doi:10.3969/j.issn.1006-267x.2022.02.059

2022 , Vol. 34 >Issue 2: 1276 - 1284

DOI: https://doi.org/10.3969/j.issn.1006-267x.2022.02.059

RESEARCH PAPER

Repair Effects of Sodium Butyrate on Inflammatory Injury of Lipopolysaccharide-Induced Bovine Mammary Epithelial Cell Line

LI Lin ,
GONG Binbin ,
XU Changfeng ,
CAO Meng ,
LI Jianyuan ,
ZHAO Mei ,
TANG Weibin

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1. School of Biological Science and Engineering, Xingtai University, Xingtai 054001, China;
2. Department of Pathology, Xingtai People's Hospital, Hebei Medical University Affiliated Hospital, Xingtai 054001, China

Received date: 2021-06-27

Online published: 2022-02-15

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Abstract

This study intended to analyze the repair effects of sodium butyrate on inflammatory injury of lipopolysaccharide (LPS)-induced bovine mammary epithelial cell line (MAC-T), and to expound the regulatory mechanism of sodium butyrate on mammary gland health of dairy cows in vitro. The oxidative damage model was established by adding different concentrations (0, 1, 10, 100, 1 000 and 10 000 ng/mL) of LPS into MAC-T cells and determining the cell viability, to determine the appropriate concentration of LPS. In order to determine the appropriate concentration of sodium butyrate, the apoptosis rate of cells was detected by adding different concentrations (0, 2, 4, 8, 16 and 32 μmol/L) of sodium butyrate into MAC-T cells. Finally, 1 000 ng/mL LPS and 16 μmol/L sodium butyrate were selected for this experiment. The experiment was divided into three groups:control group, LPS treatment group and LPS+sodium butyrate treatment group, and the cells morphology, oxidative stress indexes and of apoptosis protein mRNA expression levels were detected. The results showed as follows:1) the cells in the control group showed a flat, irregular shape and were well attached; the cells in the LPS treatment group had pyknosis and rupture of the nuclei, and a large area of death and shedding occurred; the cell edges in the LPS+sodium butyrate treatment group were clear, the intracellular particles were less, and the death and exfoliation were reduced obviously. 2) Compared with the control group, the superoxide dismutase (SOD) activity and total antioxidant stress capacity (T-AOC) in MAC-T cells of LPS treatment group were significantly decreased (P<0.05), and the malondialdehyde (MDA) content was significantly increased (P<0.05); compared with the LPS treatment group, the SOD activity and T-AOC in MAC-T cells of LPS+sodium butyrate treatment group were significantly increased (P<0.05), and the MDA content was significantly decreased (P<0.05). 3) Compared with the control group, the mRNA expression levels of cysteinyl aspartate specific proteinase-3 (Caspase-3), cysteinyl aspartate specific proteinase-9 (Caspase-9) and B-cell lymphoma/leukaemia-2-associated X protein (Bax) in MAC-T cells of LPS+sodium butyrate treatment group were significantly increased (P<0.05 or P<0.01), and the B-cell lymphoma/leukaemia-2 (Bcl-2) mRNA expression level was significantly decreased (P<0.05); compared with the LPS treatment group, the mRNA expression levels of Caspase-3 and Caspase-9 in MAC-T cells of LPS+sodium butyrate treatment group were significantly decreased (P<0.05), and the Bcl-2 mRNA expression level was significantly increased (P<0.05). In conclusion, sodium butyrate plays a certain role in repairing the oxidative damage of MAC-T cells caused by LPS and reduces the occurrence of cell apoptosis.

Key words： sodium butyrate; LPS; MAC-T cells; oxidative stress; cell apoptosis

Cite this article

LI Lin , GONG Binbin , XU Changfeng , CAO Meng , LI Jianyuan , ZHAO Mei , TANG Weibin . Repair Effects of Sodium Butyrate on Inflammatory Injury of Lipopolysaccharide-Induced Bovine Mammary Epithelial Cell Line[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1276 -1284 . DOI: 10.3969/j.issn.1006-267x.2022.02.059

References

[1] 陈志, 张逸, 路钦越, 等.茶树油对LPS诱导的奶牛乳腺炎的作用及其机制[J].中国农业科学, 2021, 54(14):3124-3133. CHEN Z, ZHANG Y, LU Q Y, et al.Effect and mechanism of tea tree oil on LPS induced mastitis in dairy cows[J].Scientia Agricultura Sinica, 2021, 54(14):3124-3133.(in Chinese)
[2] 朱智, 纪邑奇, 黄文明, 等.丁酸在亚急性瘤胃酸中毒和动物健康方面作用机制的研究进展[J].动物营养学报, 2021, 33(8):4201-4212. ZHU Z, JI Y Q, HUANG W M, et al.Research progress on mechanism of butyrate in subacute ruminal acidosis and animal health[J].Chinese Journal of Animal Nutrition, 2021, 33(8):4201-4212.(in Chinese)
[3] DAI H Y, LIU X X, YAN J Y, et al.Sodium butyrate ameliorates high-concentrate diet-induced inflammation in the rumen epithelium of dairy goats[J].Journal of Agricultural and Food Chemistry, 2017, 65(3):596-604.

[4] 刘文慧, 阿拉腾珠拉, 马露, 等.丁酸钠与丝兰对大肠杆菌K99攻毒哺乳犊牛生长性能及血清抗氧化指标的影响[J].动物营养学报, 2020, 32(9):4177-4184. LIU W H, A L T Z L, MA L, et al.Effects of sodium butyrate and yucca on growth performance and serum antioxidant indices of sucking calves challenged with Escherichia coli K99[J].Chinese Journal of Animal Nutrition, 2020, 32(9):4177-4184.(in Chinese)
[5] 刘馨忆.包被丁酸钠对肉仔鸡生长性能、免疫功能及肠道组织形态的影响[J].饲料研究, 2020, 43(6):41-44. LIU X Y.Effect of sodium butyrate on growth performance, immune function and intestinal morphology of broilers[J].Feed Research, 2020, 43(6):41-44.(in Chinese)
[6] QAISRANI S N, VAN KRIMPEN M M, KWAKKEL R P, et al.Diet structure, butyric acid, and fermentable carbohydrates influence growth performance, gut morphology, and cecal fermentation characteristics in broilers[J].Poultry Science, 2015, 94(9):2152-2164.

[7] 王爱华.丁酸钠的生理功能及其在畜禽生产中的应用[J].中国饲料, 2014(24):23-26. WANG A H.Physiologic function of sodium butyrate and its application in livestock and poultry production[J].China Feed, 2014(24):23-26.(in Chinese)
[8] ABDEL-LATIF H M R, ABDEL-TAWWAB M, DAWOOD M A O, et al.Benefits of dietary butyric acid, sodium butyrate, and their protected forms in aquafeeds:a review[J].Reviews in Fisheries Science & Aquaculture, 2020, 28(4):421-448.

[9] 洪键, 贾逸敏, 赵茹茜.丁酸通过增强肝线粒体功能缓解高脂诱导的小鼠肥胖[J].中国生物化学与分子生物学报, 2017, 33(12):1266-1273. HONG J, JIA Y M, ZHAO R Q.Butyrate alleviates high fat diet-induced obesity through enhancement of mitochondrial function in the liver of mice[J].Chinese Journal of Biochemistry and Molecular Biology, 2017, 33(12):1266-1273.(in Chinese)
[10] MA X M, ZHOU Z H, ZHANG X J, et al.Sodium butyrate modulates gut microbiota and immune response in colorectal cancer liver metastatic mice[J].Cell Biology and Toxicology, 2020, 36(5):509-515.

[11] ZHANG R Y, ZHU W Y, JIANG L S, et al.Comparative metabolome analysis of ruminal changes in Holstein dairy cows fed low- or high-concentrate diets[J].Metabolomics, 2017, 13(6):74.
[12] DENNIS T S, SUAREZ-MENA F X, HILL T M, et al.Short communication:effect of replacing corn with beet pulp in a high concentrate diet fed to weaned Holstein calves on diet digestibility and growth[J].Journal of Dairy Science, 2018, 101(1):408-412.

[13] SAMO S P, MALHI M, KACHIWAL A B, et al.Supranutritional selenium level minimizes high concentrate diet-induced epithelial injury by alleviating oxidative stress and apoptosis in colon of goat[J].BMC Veterinary Research, 2020, 16(1):462.
[14] 王来来, 张文文, 张欢敏, 等.丁酸钠对高精料日粮模式下奶山羊乳腺内组胺介导的炎症反应的影响[J].畜牧与兽医, 2017, 49(9):111-116. WANG L L, ZHANG W W, ZHANG H M, et al.Effect of high-concentrate diet feeding and dietary sodium butyrate addition on inflammatory response mediated by histamine in the mammary gland of lactating goats[J].Animal Husbandry & Veterinary Medicine, 2017, 49(9):111-116.(in Chinese)
[15] 张文文, 王来来, 代宏宇, 等.高精料日粮对奶山羊乳腺组织NOD1炎性信号通路的影响[J].畜牧与兽医, 2017, 49(9):101-106. ZHANG W W, WANG L L, DAI H Y, et al.Effect of high-concentrate diet on NOD1 inflammatory signaling pathway in the mammary glands of lactating goats[J].Animal Husbandry & Veterinary Medicine, 2017, 49(9):101-106.(in Chinese)
[16] 苗晋锋, 马海田, 邹思湘, 等.内毒素对山羊乳腺组织中与乳腺炎相关的酶和细胞因子的影响[J].福建农林大学学报(自然科学版), 2007, 36(6):608-613. MIAO J F, MA H T, ZOU S X, et al.Effect of endotoxin on enzymes and cytokines in mammary tissue of goats relating with mastitis[J].Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2007, 36(6):608-613.(in Chinese)
[17] WU X X, HUANG X L, CHEN R R, et al.Paeoniflorin prevents intestinal barrier disruption and inhibits lipopolysaccharide (LPS)-induced inflammation in caco-2 cell monolayers[J].Inflammation, 2019, 42(6):2215-2225.

[18] WANG K, LIU X Q, XIAO H, et al.The correlation between inflammatory injury induced by LPS and RAS in EpH4-Ev cells[J].International Immunopharmacology, 2017, 46:23-30.
[19] ZHANG L L, HOU X, SUN L C, et al.Staphylococcus aureus bacteriophage suppresses LPS-induced inflammation in MAC-T bovine mammary epithelial cells[J].Frontiers in Microbiology, 2018, 9:1614.
[20] ZHANG W H, GAO F, ZHU Q F, et al.Dietary sodium butyrate alleviates the oxidative stress induced by corticosterone exposure and improves meat quality in broiler chickens[J].Poultry Science, 2011, 90(11):2592-2599.

[21] ZHOU D, PAN Q, XIN F Z, et al.Sodium butyrate attenuates high-fat diet-induced steatohepatitis in mice by improving gut microbiota and gastrointestinal barrier[J].World Journal of Gastroenterology, 2017, 23(1):60-75.

[22] QIU Y Q, MA X Y, YANG X F, et al.Effect of sodium butyrate on cell proliferation and cell cycle in porcine intestinal epithelial (IPEC-J2) cells[J].In Vitro Cellular & Developmental Biology.Animal, 2017, 53(4):304-311.

[23] WANG Y, BRANICKY R, NOË A, et al.Superoxide dismutases:dual roles in controlling ROS damage and regulating ROS signaling[J].Journal of Cell Biology, 2018, 217(6):1915-1928.

[24] MOLONEY J N, COTTER T G.ROS signalling in the biology of cancer[J].Seminars in Cell & Developmental Biology, 2018, 80:50-64.
[25] CLÉMENT M V, PERVAIZ S.Reactive oxygen intermediates regulate cellular response to apoptotic stimuli:an hypothesis[J].Free Radical Research, 1999, 30(4):247-252.

[26] SALIMI V, SHAHSAVARI Z, SAFIZADEH B, et al.Sodium butyrate promotes apoptosis in breast cancer cells through reactive oxygen species (ROS) formation and mitochondrial impairment[J].Lipids in Health and Disease, 2017, 16(1):208.
[27] SUN B, JIA Y M, YANG S, et al.Sodium butyrate protects against high-fat diet-induced oxidative stress in rat liver by promoting expression of nuclear factor E2-related factor 2[J].The British Journal of Nutrition, 2019, 122(4):400-410.

[28] LUDWIG S, PLESCHKA S, PLANZ O, et al.Ringing the alarm bells:signalling and apoptosis in influenza virus infected cells[J].Cellular Microbiology, 2006, 8(3):375-386.

[29] ZHOU Z H, XU N B, MATEI N, et al.Sodium butyrate attenuated neuronal apoptosis via GPR41/Gβγ/PI3K/Akt pathway after MCAO in rats[J].Journal of Cerebral Blood Flow and Metabolism, 2021, 41(2):267-281.

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