RESEARCH PAPER

Inhibitory Effect of Curcumin against Rotavirus Infection in Intestinal Porcine Epithelial Cells

  • ZHUO Ruhao ,
  • WU Jiamin ,
  • XU Menghui ,
  • TAO Shengxiang ,
  • MENG Chuang ,
  • ZHONG Xiang
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  • 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China;
    2. Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infection Diseases and Zoonoses, Yangzhou 225009, China

Received date: 2021-06-29

  Online published: 2022-02-15

Abstract

This study was explored the antiviral effect of curcumin on porcine rotavirus (PRV)-infected intestinal porcine epithelial cells (IPEC-J2 cells). IPEC-J2 cells were used as the experimental object and were assigned to the negative control group, the PRV infection (multiplicity of infection=0.1) group, and the PRV infection with curcumin treatment (20 μmol/L) group, respectively. After PRV infection, the cytopathic changes were observed, and the content of reactive oxygen species in IPEC-J2 cells was detected by flow cytometry, and the replication and proliferation of PRV in IPEC-J2 cells were detected by real-time fluorescence quantification(qRT-PCR) and virus titer assay. The results showed as follows:compared with a negative control group, 1) PRV infection caused lesions of IPEC-J2 cells, significantly reduced cell viability (P<0.05), and significantly upregulated the content of intracellular ROS (P<0.01); 2) curcumin could inhibit the replication and proliferation of PRV in IPEC-J2 cells (P<0.05); 3) adding curcumin at the stage of PRV adsorption cells significantly inhibited the replication and proliferation of the virus (P<0.05); 4) direct incubation of curcumin with PRV before infection could significantly reduce the titer of the virus after infection (P<0.05); 5) PRV infection significantly increased the mRNA relative expression levels of melanoma differentiation related gene 5, interferon-induced protein 44-like protein antibody and interferon-β (P<0.05), and significantly reduced the mRNA relative expression levels of Toll-like receptor adapter molecule 1, mitochondrial antiviral signaling protein and myeloid differentiation factor 88 in IPEC-J2 cells (P<0.05), however, adding curcumin could significantly reduce or increase the expression of these immune gene affected by the virus (P<0.05). In summary, this study confirmed for the first time that curcumin can inhibit PRV infection and replication in intestinal porcine epithelial cells, providing important approach for prevention and treatment of piglet viral diarrhea.

Cite this article

ZHUO Ruhao , WU Jiamin , XU Menghui , TAO Shengxiang , MENG Chuang , ZHONG Xiang . Inhibitory Effect of Curcumin against Rotavirus Infection in Intestinal Porcine Epithelial Cells[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1285 -1295 . DOI: 10.3969/j.issn.1006-267x.2022.02.060

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