RESEARCH PAPER

Isolation and Identification of Cadmium-Tolerant Lactobacillus Strains from Chicken

  • PU Junhua ,
  • LIU Yinyin ,
  • CHEN Dawei ,
  • GAO Yushi ,
  • LU Junxian ,
  • ZHANG Xiaoyan ,
  • TANG Mengjun ,
  • ZHOU Qian ,
  • KONG Lingwu
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  • 1. Jiangsu Institute of Poultry Sciences, Yangzhou 225125, China;
    2. Yangzhou Shuangyang Biotechnology Co., Ltd., Yangzhou 225124, China

Received date: 2021-07-11

  Online published: 2022-02-15

Abstract

The aim of this experiment was to isolate and screen cadmium(Cd)-tolerant Lactobacillus from the chicken intestine, and to provide a theoretical basis for the prevention and control of Cd contamination used in poultry feed. Lactobacillus, which was isolated and screened from the samples of chicken intestinal contents by resisting 100 mg/L Cd, was identified by molecular biological methods. The growth properties, Cd resistance and Cd adsorption properties were determined on the selected lactic acid strains. The results showed as follows:five Cd-tolerant Lactobacillus strains were identified, including one strain of Lactobacillus johnsonii Cd3-1 and four strains of Lactobacillus crispatus:Cd1-1, Cd5-2, Cd4-5 and Cd8-2. Among these strains, Cd3-1, Cd5-2 and Cd8-2 strains were tolerant up to 1 000 mg/L of Cd while Cd1-1 and Cd4-5 strains were tolerant to 400 mg/L of Cd. The absorption rate of Cd in 50 mg/L Cd solution raised with the increase of pH in the solution, and the Cd absorption rate of each strain in Cd solution of pH 6.0 and 7.0 was significantly higher than that of in Cd solution of pH 2.6. All five Lactobacillus strains were resistant to 0.3% bile salts and inhibited the growth of Staphylococcus aureus. In conclusion, five Cd-tolerant Lactobacillus strains, including one strain of Lactobacillus johnsoni and four strains of Lactobacillus crispatus, are selected from the chicken intestine and their adsorption rates of Cd are up to 30% under the pH close to neutral condition.

Key words: chicken; cadmium; Lactobacillus

Cite this article

PU Junhua , LIU Yinyin , CHEN Dawei , GAO Yushi , LU Junxian , ZHANG Xiaoyan , TANG Mengjun , ZHOU Qian , KONG Lingwu . Isolation and Identification of Cadmium-Tolerant Lactobacillus Strains from Chicken[J]. Chinese Journal of Animal Nutrition, 2022 , 34(2) : 1305 -1312 . DOI: 10.3969/j.issn.1006-267x.2022.02.062

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