[1] BOISEN S, FERNÁNDEZ J A.Prediction of the apparent ileal digestibility of protein and amino acids in feedstuffs and feed mixtures for pigs by
in vitro analyses[J].Animal Feed Science and Technology, 1995, 51(1/2):29-43.
[2] BRYAN D D S L, ABBOTT D A, CLASSEN H L.Digestion kinetics of protein sources determined using an
in vitro chicken model[J].Animal Feed Science and Technology, 2019, 248:106-113.
[3] BOISEN S.
In vitro analyses for predicting standardised ileal digestibility of protein and amino acids in actual batches of feedstuffs and diets for pigs[J].Livestock Science, 2007, 109(1/3):182-185.
[4] ROCHELL S J, KUHLERS D L, DOZIER W A 3RD.Relationship between
in vitro assays and standardized ileal amino acid digestibility of animal protein meals in broilers[J].Poultry Science, 2013, 92(1):158-170.

[5] SHEEN H, ALI-KHAN Z.Protein sample concentration by repeated loading onto SDS-PAGE[J].Analytical Biochemistry, 2005, 343(2):338-340.

[6] KIES A K, DE JONGE L H, KEMME P A, et al.Interaction between protein, phytate, and microbial phytase.
In vitro studies[J].Journal of Agricultural and Food Chemistry, 2006, 54(5):1753-1758.

[7] SADEGHI A A, NIKKHAH A, SHAWRANG P, et al.Protein degradation kinetics of untreated and treated soybean meal using SDS-PAGE[J].Animal Feed Science and Technology, 2006, 126(1/2):121-133.
[8] ARTE E, RIZZELLO C G, VERNI M, et al.Impact of enzymatic and microbial bioprocessing on protein modification and nutritional properties of wheat bran[J].Journal of Agricultural and Food Chemistry, 2015, 63(39):8685-8693.

[9] THOMAS K, AALBERS M, BANNON G A, et al.A multi-laboratory evaluation of a common
in vitro pepsin digestion assay protocol used in assessing the safety of novel proteins[J].Regulatory Toxicology and Pharmacology, 2004, 39(2):87-98.

[10] BRYAN D D S L, ABBOTT D A, CLASSEN H L.Development of an
in vitro protein digestibility assay mimicking the chicken digestive tract[J].Animal Nutrition, 2018, 4(4):401-409.

[11] ROSA-SIBAKOV N, RE M, KARSMA A, et al.Phytic acid reduction by bioprocessing as a tool to improve the
in vitro digestibility of faba bean protein[J].Journal of Agricultural and Food Chemistry, 2018, 66(40):10394-10399.

[12] SOUSA R, PORTMANN R, DUBOIS S, et al.Protein digestion of different protein sources using the INFOGEST static digestion model[J].Food Research International, 2020, 130:108996.
[13] 党方坤.生长猪肠内消化酶的纯化与肠液体外模拟的研究[D].硕士学位论文.北京:中国农业科学院, 2018. DANG F K.Study on purification of digestive enzymes in intestinal tract and in vitro simulation of intestinal fluid for growing pigs[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2018.(in Chinese)
[14] DAHLQVIST A.A method for the determination of amylase in intestinal content[J].Scandinavian Journal of Clinical & Laboratory Investigation, 1962, 14(2):145-151.

[15] RICK W.Chymotrypsin:measurements with
N-benzoyl-
L-tyrosine ethyl ester as substrate[M]//BERGMEYER H U.Methods of enzymatic analysis.2nd ed.Pittsburgh:Academic Press, 1974:1009-1012.
[16] RICK W.Trypsin:measurement with
Nα-
p-toluenesulfonyl-
L-arginine methyl ester as substrate[M]//BERGMEYER H U.Methods of enzymatic analysis.2nd ed.Pittsburgh:Academic Press, 1974:1021-1024.
[17] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.饲料中水分的测定:GB/T 6435-2014[S].北京:中国标准出版社, 2014. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.Determination of moisture in feedstuffs:GB/T 6435-2014[S].Beijing:Standards Press of China, 2014.(in Chinese)
[18] 国家市场监督管理总局, 国家标准化管理委员会.饲料中粗蛋白的测定 凯氏定氮法:GB/T 6432-2018[S].北京:中国标准出版社, 2018. State Administration for Market Regulation, Standardization Administration of the People's Republic of China.Determination of crude protein in feeds-Kjeldahl method:GB/T 6432-2018[S].Beijing:Standards Press of China, 2018.(in Chinese)
[19] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.饲料中粗脂肪的测定:GB/T 6433-2006[S].北京:中国标准出版社, 2006. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.Determinaiion of crude fat in feeds:GB/T 6433-2006[S].Beijing:Standards Press of China, 2006.(in Chinese)
[20] 中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.饲料中中性洗涤纤维 (NDF) 的测定:GB/T 20806-2006[S].北京:中国标准出版社, 2006. General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.Determination of neutral detergent fiber in feedstuffs:GB/T 20806-2006[S].Beijing:Standards Press of China, 2006.(in Chinese)
[21] 中华人民共和国农业部.饲料中酸性洗涤纤维的测定:NY/T 1459-2007[S].北京:农业出版社, 2007. The ministry of agriculture of the People's Republic of China.Determination of acid detergent fiber in feedstuff (ADF):NY/T 1459-2007[S].Beijing:Agricultural Publishing House, 2007.(in Chinese)
[22] International Organization for Standardization.Animal feeding stuffs, animal products, and faeces or urine-determination of gross calorific value-Bomb calorimeter method:US ISO 9831:1998[S].Geneva:ISO, 1998.
[23] ZHAO F, REN L Q, MI B M, et al.Developing a computer-controlled simulated digestion system to predict the concentration of metabolizable energy of feedstuffs for rooster[J].Journal of Animal Science, 2014, 92(4):1537-1547.

[24] 傅淑宏, 魏开华, 李晓霞, 等.胃腺癌患者粪便蛋白质的提取及初步质谱分析[J].现代检验医学杂志, 2015(4):14-20. FU S H, WEI K H, LI X X, et al.Protein extraction and preliminary research on proteins in stools from patients with gastric adenocarcinoma[J].Journal of Modern Laboratory Medicine, 2015(4):14-20.(in Chinese)
[25] 彭运智, 谭会泽, 刘松柏, 等.基于仿生消化系统估测肉鸭饲料原料代谢能的研究[J].动物营养学报, 2020, 32(2):881-889. PENG Y Z, TAN H Z, LIU S B, et al.A study of feed ingredient metabolisable energy of meat ducks predicted with simulated digestion system[J].Chinese Journal of Animal Nutrition, 2020, 32(2):881-889.(in Chinese)
[26] 魏杰, 谢明, 赵峰, 等.豆粕的鸭代谢能与酶水解能相关性研究[C]//中国畜牧兽医学会2018年学术年会禽病学分会第十九次学术研讨会论文集.南宁:中国畜牧兽医学会, 2018:311. WEI J, XIE M, ZHAO F, et al.Correlation between metabolizable energy and enzymatic hydrolysis energy of soybean meal in ducks[C]//Proceedings of the 19th Symposium of Avian Epidemiology Branch of the Chinese Society of Animal Science and Veterinary Medicine.Nanning:Chinese Association of Animal Science and Veterinary Medicine, 2018:311.(in Chinese)
[27] 张莉, 赵峰, 卞国志, 等.两种模拟肠液在仿生消化法估测鸭饲料能量消化率中的比较研究[C]//中国畜牧兽医学会动物营养学分会第七届中国饲料营养学术研讨会论文集.郑州:中国畜牧兽医学会动物营养学分会, 2014:254-255. ZHANG L, ZHAO F, BIAN G Z, et al.Comparative study of two kinds of simulated intestinal fluids in estimating energy digestibility of duck feed by bionic digestion method[C]//Proceedings of the 7th Chinese Feed Nutrition Academic Symposium, Animal Nutrition Branch, Chinese Society of Animal Science and Veterinary Medicine.Zhengzhou:Animal Nutrition Branch of China Institute of Animal Husbandry and Veterinary Medicine, 2014:254-255.(in Chinese)
[28] 王亚.基于模拟生长猪胃-小肠消化过程测定谷实类饲料原料蛋白质效价的研究[D].硕士学位论文.北京:中国农业科学院, 2019. WANG Y.Study on determination of protein availability in cereal g rains based on simulated gastro-small intestinal digestion process for growing pigs[D].Master's Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2019.(in Chinese)
[29] DUODU K G, TAYLOR J R N, BELTON P S, et al.Factors affecting sorghum protein digestibility[J].Journal of Cereal Science, 2003, 38(2):117-131.

[30] COLLIER K, BARBER L, LOTT J N A.A study of indigestible protein fractions of rice (
Oryza sativa, L.) endosperm fed to mice (
Mus musculus) and sheep (
Ovis musimon):a qualitative and quantitative analysis[J].Journal of Cereal Science, 1998, 27(1):95-101.

[31] NURFAIDAH, METUSALACH, MAHENDRADATTA M, et al.Analysis of molecular weight albumin concentrate on various types of freshwater fish using SDS-page electrophoresis method[J].IOP Conference Series:Earth and Environmental Science, 2020, 564:012057.
[32] ELKIN R G, FREED M B, HAMAKER B R, et al.Condensed tannins are only partially responsible for variations in nutrient digestibilities of sorghum grain cultivars[J].Journal of Agricultural and Food Chemistry, 1996, 44(3):848-853.

[33] VALDES E V, LEESON S.Measurement of metabolizable energy in poultry feeds by an
in vitro system[J].Poultry Science, 1992, 71(9):1493-1503.

[34] BOISEN S, FERNÁNDEZ J A.Prediction of the total tract digestibility of energy in feedstuffs and pig diets by
in vitro analyses[J].Animal Feed Science and Technology, 1997, 68(3/4):277-286.
[35] ZHANG L, ZHAO F, ZHANG H, et al.Validation of
in vitro digestion using simulated small intestinal fluid with specific digestive activity to predict the metabolizable energy of feed ingredients for duck[J].Poultry Science, 2019, 98(3):1280-1287.

[36] 任立芹.仿生法评定黄羽肉鸡常用饲料代谢能和可消化氨基酸研究[D].博士学位论文.北京:中国农业科学院, 2012. REN L Q.Study on the method to predict the metabolizable energy and digestible amino acid of ingredients in yellow chickens using the simulative digestion system[D].Ph.D.Thesis.Beijing:Chinese Academy of Agricultural Sciences, 2012.(in Chinese)